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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Nucleotidyltransferase, Transferase
Biological processNitrogen fixation
LigandMagnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:ECA1029
OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Taxonomic identifieri218491 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaePectobacterium
Proteomesi
  • UP000007966 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001927311 – 904Bifunctional uridylyltransferase/uridylyl-removing enzymeAdd BLAST904

Interactioni

Protein-protein interaction databases

STRINGi218491.ECA1029.

Structurei

3D structure databases

ProteinModelPortaliQ6D8E5.
SMRiQ6D8E5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini479 – 601HDPROSITE-ProRule annotationAdd BLAST123
Domaini720 – 801ACT 1UniRule annotationAdd BLAST82
Domaini827 – 904ACT 2UniRule annotationAdd BLAST78

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 360UridylyltransferaseAdd BLAST360
Regioni361 – 719Uridylyl-removingAdd BLAST359

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105E1P. Bacteria.
COG2844. LUCA.
HOGENOMiHOG000261778.
KOiK00990.
OMAiARICGYF.
OrthoDBiPOG091H04HO.

Family and domain databases

CDDicd00077. HDc. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf. 1 hit.
InterProiView protein in InterPro
IPR002912. ACT_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
IPR002934. Polymerase_NTP_transf_dom.
IPR010043. UTase/UR.
PANTHERiPTHR13734:SF45. PTHR13734:SF45. 1 hit.
PfamiView protein in Pfam
PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiView protein in SMART
SM00471. HDc. 1 hit.
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiView protein in PROSITE
PS51671. ACT. 2 hits.
PS51831. HD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6D8E5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDKRFSPDN TPPDASSPDS PIDTIAAAQP PVSPLTYADD MLNCQALKQQ
60 70 80 90 100
LELFQLWLGS EFRSGVSAEK LIDARTLFID RLLQRLWYCH GFENIAQTAL
110 120 130 140 150
VAVGGYGRGE LHPLSDIDVL VLSQTELSDE HSQRVGQFIT LLWDLKLEVG
160 170 180 190 200
HSVRTLEECL QEGRADISVA TNLIESRMIC GDVALFLTLQ KHVFSDEFWP
210 220 230 240 250
SPTFFPAKIT EQQERHQRYH STSYNLEPDI KSSPGGLRDI HTLLWVARRH
260 270 280 290 300
FGATSLNEMV GFGFLTEAER KELNECQSFL WRIRFALHLI LPRYDNRLLF
310 320 330 340 350
DRQLNVAQLL QYQGEGNTPV ERMMKDFYRM TRRVSELNQM LLQLFDEAIL
360 370 380 390 400
ALDASEKPRP IDDEFQLRGN LVDLRDENLF IKKPEAIMRM FYLMVRNRDI
410 420 430 440 450
SGIYSTTLRQ LRHARRHLAS PLCTIPEARQ LFMNILRHPY AVSRALLPMH
460 470 480 490 500
RHSVLWAYMP LWGNIVGQMQ FDLFHAYTVD EHTIRVLLKL ESFADEDTRP
510 520 530 540 550
QHPLCVELYP RLPQPELLLL AALFHDIAKG RGGDHSELGA LDVLEFAALH
560 570 580 590 600
GLNSREAQLV SWLVRCHLLM SVTAQRRDIQ DPTVIQQFAT EVQSETRLRY
610 620 630 640 650
LVSLTVADIC ATNETLWNSW KQSLLRELYF ATEKQLRRGM QNTPDLRERV
660 670 680 690 700
RHHRLQALAL LRMDNIDEEA LHHIWSRCRA DYFLRHSPNQ IAWHARHLLE
710 720 730 740 750
HDTNKPLVLI SHQASRGGTE IFIWSPDRPY LFAAVAGELD RRNLSVHDAQ
760 770 780 790 800
IFTSRDGMAM DTFIVLEPDG SPLAQDRHEM IRHALEQALT QRHYQHPRVR
810 820 830 840 850
RTSPKLRHFS VPTEVNFLPT HTDRRSYMEL SALDQPGLLA RIGEIFSDLN
860 870 880 890 900
LSLHGARIST IGERVEDLFI LADSDRRALK PELRLKLQER LTEALNPNDK

VPLS
Length:904
Mass (Da):104,412
Last modified:August 16, 2004 - v1
Checksum:i1A6A4DEF5361ED32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950851 Genomic DNA. Translation: CAG73940.1.
RefSeqiWP_011092628.1. NC_004547.2.

Genome annotation databases

EnsemblBacteriaiCAG73940; CAG73940; ECA1029.
KEGGieca:ECA1029.
PATRICifig|218491.5.peg.1037.

Similar proteinsi

Entry informationi

Entry nameiGLND_PECAS
AccessioniPrimary (citable) accession number: Q6D8E5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: August 16, 2004
Last modified: October 25, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families