Q6D8D0 (LPXB_ERWCT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipid-A-disaccharide synthase
EC=2.4.1.182

Gene names

Name:	lpxB
Ordered Locus Names:	ECA1044

Organism

Erwinia carotovora subsp. atroseptica (strain SCRI 1043 / ATCC BAA-672) (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

218491 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium › Erwinia carotovora subsp. atroseptica

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP-Rule MF_00392
Catalytic activity	UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate = UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate. HAMAP-Rule MF_00392
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 5/6. HAMAP-Rule MF_00392
Sequence similarities	Belongs to the LpxB family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid biosynthesis Lipid metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	lipid-A-disaccharide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 383	383	Lipid-A-disaccharide synthase HAMAP-Rule MF_00392		PRO_0000255178

Sequences

	Sequence		Length	Mass (Da)
	Q6D8D0 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 0F65CE47E3A465A6	FASTA	383	42,359
	10 20 30 40 50 60 MSSRPLTIGL VAGETSGDIL GAGLIRALKE KVPGARFVGV AGPRMQAEGC EAWYEMEELA 70 80 90 100 110 120 VMGIVEVLGR LPRLLKIRRD LTQRFSELQP DVFVGIDAPD FNITLEGNLK QHGINTIHYV 130 140 150 160 170 180 SPSVWAWRQK RVFKIGKATN LVLAFLPFEK AFYDRFNVPC RFIGHTMADA MPLHPDKMTA 190 200 210 220 230 240 RATLGIAPDA HCLALLPGSR GAEVEMLSAD FLNTAVLLRQ HFPDLEIVVP LVNSKRREQF 250 260 270 280 290 300 ERIKSSVAPD LRVHLLDGQA REAMIASDAA LLASGTAALE CMLAKCPMVV GYRMKPFTFW 310 320 330 340 350 360 LAQRLVKTPW VSLPNLLAGR ELVTELLQTD CTPDKLAAAL LPLFADTDKM AELRTTFVDL 370 380 HQQIRCNADE QAAQAVLELV TPR

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950851 Genomic DNA. Translation: CAG73955.1.
RefSeq	YP_049151.1. NC_004547.2.
3D structure databases
ProteinModelPortal	Q6D8D0.
ModBase	Search...
Protein-protein interaction databases
STRING	218491.ECA1044.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAG73955; CAG73955; ECA1044.
GeneID	2882740.
KEGG	eca:ECA1044.
PATRIC	20477304. VBIPecAtr54885_1052.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0763.
HOGENOM	HOG000018003.
KO	K00748.
OMA	PTVWAWR.
ProtClustDB	PRK00025.
Enzyme and pathway databases
BioCyc	PATR218491:GJNB-1075-MONOMER.
UniPathway	UPA00359; UER00481.
Family and domain databases
HAMAP	MF_00392. LpxB.
InterPro	IPR003835. Glyco_trans_19. [Graphical view]
Pfam	PF02684. LpxB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00215. lpxB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXB_ERWCT

Accession

Primary (citable) accession number: Q6D8D0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program