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Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase (lpxD)
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciPATR218491:G1G3T-1082-MONOMER
UniPathwayiUPA00359; UER00481

Protein family/group databases

CAZyiGT19 Glycosyltransferase Family 19

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthaseUniRule annotation (EC:2.4.1.182UniRule annotation)
Gene namesi
Name:lpxBUniRule annotation
Ordered Locus Names:ECA1044
OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Taxonomic identifieri218491 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaePectobacterium
Proteomesi
  • UP000007966 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002551781 – 383Lipid-A-disaccharide synthaseAdd BLAST383

Interactioni

Protein-protein interaction databases

STRINGi218491.ECA1044

Structurei

3D structure databases

ProteinModelPortaliQ6D8D0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D0V Bacteria
COG0763 LUCA
HOGENOMiHOG000018003
KOiK00748
OMAiPTVWAWR
OrthoDBiPOG091H01NW

Family and domain databases

HAMAPiMF_00392 LpxB, 1 hit
InterProiView protein in InterPro
IPR003835 Glyco_trans_19
PANTHERiPTHR30372 PTHR30372, 1 hit
PfamiView protein in Pfam
PF02684 LpxB, 1 hit
TIGRFAMsiTIGR00215 lpxB, 1 hit

Sequencei

Sequence statusi: Complete.

Q6D8D0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSRPLTIGL VAGETSGDIL GAGLIRALKE KVPGARFVGV AGPRMQAEGC
60 70 80 90 100
EAWYEMEELA VMGIVEVLGR LPRLLKIRRD LTQRFSELQP DVFVGIDAPD
110 120 130 140 150
FNITLEGNLK QHGINTIHYV SPSVWAWRQK RVFKIGKATN LVLAFLPFEK
160 170 180 190 200
AFYDRFNVPC RFIGHTMADA MPLHPDKMTA RATLGIAPDA HCLALLPGSR
210 220 230 240 250
GAEVEMLSAD FLNTAVLLRQ HFPDLEIVVP LVNSKRREQF ERIKSSVAPD
260 270 280 290 300
LRVHLLDGQA REAMIASDAA LLASGTAALE CMLAKCPMVV GYRMKPFTFW
310 320 330 340 350
LAQRLVKTPW VSLPNLLAGR ELVTELLQTD CTPDKLAAAL LPLFADTDKM
360 370 380
AELRTTFVDL HQQIRCNADE QAAQAVLELV TPR
Length:383
Mass (Da):42,359
Last modified:August 16, 2004 - v1
Checksum:i0F65CE47E3A465A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950851 Genomic DNA Translation: CAG73955.1
RefSeqiWP_011092642.1, NC_004547.2

Genome annotation databases

EnsemblBacteriaiCAG73955; CAG73955; ECA1044
KEGGieca:ECA1044
PATRICifig|218491.5.peg.1052

Similar proteinsi

Entry informationi

Entry nameiLPXB_PECAS
AccessioniPrimary (citable) accession number: Q6D8D0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: August 16, 2004
Last modified: February 28, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome