Q6D820 (QUEC_ERWCT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
7-cyano-7-deazaguanine synthase
EC=6.3.-.-

Alternative name(s):
7-cyano-7-carbaguanine synthase
PreQ(0) synthase
Queuosine biosynthesis protein QueC

Gene names

Name:	queC
Ordered Locus Names:	ECA1155

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ0) By similarity. HAMAP MF_01633
Catalytic activity	7-carboxy-7-carbaguanine + NH3 + ATP = 7-cyano-7-carbaguanine + ADP + phosphate + H2O. HAMAP MF_01633
Cofactor	Binds 1 zinc ion per subunit.
Pathway	Purine metabolism; 7-cyano-7-deazaguanine biosynthesis. HAMAP MF_01633
Sequence similarities	Belongs to the QueC family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Ligase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 231

231

7-cyano-7-deazaguanine synthase HAMAP MF_01633

PRO_0000246839

Regions

Nucleotide binding

8 – 18

11

ATP By similarity

Sites

Metal binding

188

1

Zinc

Metal binding

197

1

Zinc

Metal binding

200

1

Zinc

Metal binding

203

1

Zinc

Secondary structure

1

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231

Helix Strand Turn

Details...

Beta strand

3 – 7

5

Helix

12 – 24

13

Beta strand

26 – 38

13

Helix

40 – 53

14

Beta strand

56 – 62

7

Helix

65 – 68

4

Helix

71 – 76

6

Helix

99 – 114

16

Beta strand

117 – 120

4

Beta strand

127 – 129

3

Helix

132 – 134

3

Helix

136 – 150

15

Beta strand

155 – 157

3

Turn

159 – 162

4

Helix

165 – 174

10

Helix

178 – 184

7

Turn

194 – 197

4

Helix

201 – 215

15

Helix

217 – 228

12

Sequences

	Sequence		Length	Mass (Da)
	Q6D820 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: A6AEA71C503D42A0	FASTA	231	25,363
	10 20 30 40 50 60 MKRAVVVFSG GQDSTTCLIQ ALQDYDDVHC ITFDYGQRHR AEIEVAQELS QKLGAAAHKV 70 80 90 100 110 120 LDVGLLNELA TSSLTRDSIP VPDYDANAQG IPNTFVPGRN ILFLTLASIY AYQVGAEAVI 130 140 150 160 170 180 TGVCETDFSG YPDCRDEFVK ALNQAIVLGI ARDIRFETPL MWLNKAETWA LADYYQQLDT 190 200 210 220 230 VRYHTLTCYN GIKGDGCGQC AACHLRANGL AQYQKDAATV MASLKQKVGL R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.
[2]	"Crystal structure of hypothetical protein (yp_049261.1) from Erwinia carotovora subsp. atroseptica scri1043 at 2.40 A resolution." Joint center for structural genomics (JCSG) Submitted (FEB-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH ZINC ION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BX950851 Genomic DNA. Translation: CAG74065.1.

RefSeq

YP_049261.1. NC_004547.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PG3	X-ray	2.40	A	1-231	[»]

ProteinModelPortal

Q6D820.

SMR

Q6D820. Positions 1-230.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

2885761.

GenomeReviews

Gene locus ECA1155 in contig BX950851_GR.

KEGG

eca:ECA1155.

NMPDR

fig|218491.3.peg.2997.

PATRIC

20477538. VBIPecAtr54885_1170.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

HBG553284.

OMA

DWGYGCG.

ProtClustDB

PRK11106.

Enzyme and pathway databases

BioCyc

ECAR218491:ECA1155-MONOMER.

Family and domain databases

HAMAP

MF_01633. QueC.
[Tree]

InterPro

IPR018317. QueC.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]

Gene3D

G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.

KO

K06920.

Pfam

PF06508. ExsB. 1 hit.
[Graphical view]

PIRSF

PIRSF006293. ExsB. 1 hit.

TIGRFAMs

TIGR00364. TIGR00364. 1 hit.

ProtoNet

Search...

Entry information

Entry name

QUEC_ERWCT

Accession

Primary (citable) accession number: Q6D820

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	August 16, 2004
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program