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Protein

2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene

menH

Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC).UniRule annotation

Catalytic activityi

5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate = (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate.UniRule annotation

Pathwayi: 1,4-dihydroxy-2-naphthoate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD)
  3. 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH)
  4. o-succinylbenzoate synthase (menC), o-succinylbenzoate synthase (menC)
  5. no protein annotated in this organism
  6. 1,4-dihydroxy-2-naphthoyl-CoA synthase (menB)
  7. 1,4-dihydroxy-2-naphthoyl-CoA hydrolase (menI)
This subpathway is part of the pathway 1,4-dihydroxy-2-naphthoate biosynthesis, which is itself part of Quinol/quinone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate, the pathway 1,4-dihydroxy-2-naphthoate biosynthesis and in Quinol/quinone metabolism.

Pathwayi: menaquinone biosynthesis

This protein is involved in the pathway menaquinone biosynthesis, which is part of Quinol/quinone metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Menaquinone biosynthesis

Enzyme and pathway databases

BioCyciPATR218491:GJNB-1243-MONOMER.
UniPathwayiUPA00079.
UPA01057; UER00900.

Protein family/group databases

ESTHERierwct-q6d7w3. MenH_SHCHC.
MEROPSiS33.996.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthaseUniRule annotation (EC:4.2.99.20UniRule annotation)
Short name:
SHCHC synthaseUniRule annotation
Gene namesi
Name:menHUniRule annotation
Ordered Locus Names:ECA1212
OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Taxonomic identifieri218491 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium
Proteomesi
  • UP000007966 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2532532-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthasePRO_0000341904Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi218491.ECA1212.

Structurei

3D structure databases

ProteinModelPortaliQ6D7W3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. MenH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107J5P. Bacteria.
COG0596. LUCA.
HOGENOMiHOG000028072.
KOiK08680.
OMAiLNDWYQQ.
OrthoDBiEOG689HPM.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01660. MenH.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR022485. SHCHC_synthase_MenH.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR03695. menH_SHCHC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6D7W3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHGAVAPIQ PWLVCLHGLF GSGEDWSPVL PFFRDWPMLL VDLPGHGASR
60 70 80 90 100
AITTADFAEV SRQLTATLLE QGIERYWLLG YSLGGRIAMY HACEGHHDGM
110 120 130 140 150
LGLLVEGGHP GLATPEQRTE RIHHDARWAQ RFRQDPLPEA LQDWYQQAVF
160 170 180 190 200
ADIDSVQREQ LIARRSANHG ASVAAMLEAT SLGRQPFLAA RLQHLSIPFI
210 220 230 240 250
YLCGASDVKF QTLAAQYGLP LLSVALAGHN AHQANPAAYA ERVRTFLSHP

VKD
Length:253
Mass (Da):27,941
Last modified:August 16, 2004 - v1
Checksum:i0A8D8225AC836E09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950851 Genomic DNA. Translation: CAG74122.1.

Genome annotation databases

EnsemblBacteriaiCAG74122; CAG74122; ECA1212.
KEGGieca:ECA1212.
PATRICi20477662. VBIPecAtr54885_1232.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950851 Genomic DNA. Translation: CAG74122.1.

3D structure databases

ProteinModelPortaliQ6D7W3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi218491.ECA1212.

Protein family/group databases

ESTHERierwct-q6d7w3. MenH_SHCHC.
MEROPSiS33.996.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG74122; CAG74122; ECA1212.
KEGGieca:ECA1212.
PATRICi20477662. VBIPecAtr54885_1232.

Phylogenomic databases

eggNOGiENOG4107J5P. Bacteria.
COG0596. LUCA.
HOGENOMiHOG000028072.
KOiK08680.
OMAiLNDWYQQ.
OrthoDBiEOG689HPM.

Enzyme and pathway databases

UniPathwayiUPA00079.
UPA01057; UER00900.
BioCyciPATR218491:GJNB-1243-MONOMER.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01660. MenH.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR022485. SHCHC_synthase_MenH.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR03695. menH_SHCHC. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SCRI 1043 / ATCC BAA-672.

Entry informationi

Entry nameiMENH_PECAS
AccessioniPrimary (citable) accession number: Q6D7W3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: August 16, 2004
Last modified: May 11, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.