Q6D7J7 (SYQ_PECAS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine--tRNA ligase
EC=6.1.1.18

Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	ECA1328

Organism

Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica) [Complete proteome] [HAMAP]

Taxonomic identifier

218491 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium › Pectobacterium atrosepticum

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00126
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00126.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: UniProtKB-HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: UniProtKB-HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 552	552	Glutamine--tRNA ligase HAMAP-Rule MF_00126		PRO_0000242869
Regions
	Motif	34 – 44	11	"HIGH" region HAMAP-Rule MF_00126
	Motif	268 – 272	5	"KMSKS" region HAMAP-Rule MF_00126
Sites
	Binding site	271	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6D7J7 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 57828D6FD0034782	FASTA	552	63,182
	10 20 30 40 50 60 MSEAEARPTN FIRQIIDEDL ASGKHDHIQT RFPPEPNGYL HIGHAKSICL NFGIARDYQG 70 80 90 100 110 120 QCNLRFDDTN PVKEDIEYVE SIKRDVEWLG FSWSGDVRYS SDYFDQLHAY AVELIGKGLA 130 140 150 160 170 180 YVDELTPEQI REYRGTLTAP GKNSPYRDRT VQENLALFEK MRNGGFAEGT ACLRAKIDMA 190 200 210 220 230 240 SSFIVMRDPV LYRIKFADHH QTGNKWCIYP MYDFTHCISD ALEGITHSLC TLEFQDNRRL 250 260 270 280 290 300 YDWVLDNISI PCHPRQYEFS RLNLEYAIMS KRKLNQLVVE NVVEGWDDPR MPTISGLRRR 310 320 330 340 350 360 GYSASSIREF CVRIGVTKQD NNVEMAALES CIRDDLNENA PRAMAVLDPV KVVIENLPAG 370 380 390 400 410 420 HEEFVAMPNH PNKPEMGSRQ VAFSREVYID RADFREEANK QYKRLVLGKE VRLRNAYVIK 430 440 450 460 470 480 ADRIEKDEQG TITTIYCSYD AETLSKDPAD GRKVKGVIHW VSAAHAVPAE FRLYDRLFSV 490 500 510 520 530 540 ANPAAADDFL STINPDSLKI TQGFVEASLV QAEVEKAYQF EREGYFCADR VYSSAEHLVF 550 NRTVGLRDTW VG

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950851 Genomic DNA. Translation: CAG74238.1.
RefSeq	YP_049434.1. NC_004547.2.
3D structure databases
ProteinModelPortal	Q6D7J7.
SMR	Q6D7J7. Positions 9-549.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	218491.ECA1328.
Proteomic databases
PRIDE	Q6D7J7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAG74238; CAG74238; ECA1328.
GeneID	2881330.
KEGG	eca:ECA1328.
PATRIC	20477924. VBIPecAtr54885_1357.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000259232.
KO	K01886.
OMA	VTHSICT.
OrthoDB	EOG6DRPF7.
ProtClustDB	PRK05347.
Enzyme and pathway databases
BioCyc	PATR218491:GJNB-1365-MONOMER.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 2.40.240.10. 2 hits. 3.40.50.620. 2 hits.
HAMAP	MF_00126. Gln_tRNA_synth.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth. IPR022861. Gln_tRNA_ligase_bac. IPR000924. Glu/Gln-tRNA-synth. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. SSF50715. 1 hit.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_PECAS

Accession

Primary (citable) accession number: Q6D7J7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	August 16, 2004
Last modified:	April 16, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program