Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Endonuclease 8

Gene

nei

Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Schiff-base intermediate with DNAUniRule annotation1
Active sitei3Proton donorUniRule annotation1
Active sitei53Proton donor; for beta-elimination activityUniRule annotation1
Binding sitei70DNAUniRule annotation1
Binding sitei125DNAUniRule annotation1
Binding sitei169DNAUniRule annotation1
Active sitei253Proton donor; for delta-elimination activityUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri229 – 263FPG-typeUniRule annotationAdd BLAST35

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Glycosidase, Hydrolase, Lyase
Biological processDNA damage, DNA repair
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 8UniRule annotation
Alternative name(s):
DNA glycosylase/AP lyase NeiUniRule annotation (EC:3.2.2.-UniRule annotation, EC:4.2.99.18UniRule annotation)
DNA-(apurinic or apyrimidinic site) lyase NeiUniRule annotation
Endonuclease VIIIUniRule annotation
Gene namesi
Name:neiUniRule annotation
Ordered Locus Names:ECA1355
OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Taxonomic identifieri218491 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaePectobacterium
Proteomesi
  • UP000007966 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedUniRule annotation
ChainiPRO_00001708962 – 263Endonuclease 8Add BLAST262

Interactioni

Protein-protein interaction databases

STRINGi218491.ECA1355.

Structurei

3D structure databases

ProteinModelPortaliQ6D7H0.
SMRiQ6D7H0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FPG family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri229 – 263FPG-typeUniRule annotationAdd BLAST35

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG4108S0J. Bacteria.
COG0266. LUCA.
HOGENOMiHOG000020882.
KOiK05522.
OMAiEILWQAQ.
OrthoDBiPOG091H01RH.

Family and domain databases

HAMAPiMF_01253. Endonuclease_8. 1 hit.
InterProiView protein in InterPro
IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR023713. Endonuclease-VIII.
IPR012319. FPG_cat.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_FPG/IleRS.
PfamiView protein in Pfam
PF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
SMARTiView protein in SMART
SM00898. Fapy_DNA_glyco. 1 hit.
SM01232. H2TH. 1 hit.
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEiView protein in PROSITE
PS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6D7H0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEGPEIRRA ADKLVEAVVG KTLTRVWFAF PELKPYETEL VGQQVRQIET
60 70 80 90 100
RGKALLTYFS HDRVLYSHNQ LYGVWRVVNA GESPETKRDL RIRLETQDRA
110 120 130 140 150
ILLYSASDIE MLTLDTLTAH PFLQRIGPDV LDLSLTPEQV CERLLLPRFR
160 170 180 190 200
RRQFSGLLLD QAFLAGLGNY LRVEILWQAQ LAPQHTASQL NEEQLQTLSR
210 220 230 240 250
ALLEIPRLSY NTRGTVDENR HHGAIFSFKV FHREGESCER CGGTIERTML
260
SSRPFYWCPH CQS
Length:263
Mass (Da):30,336
Last modified:January 23, 2007 - v3
Checksum:iD5117C39CE824EE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950851 Genomic DNA. Translation: CAG74265.1.
RefSeqiWP_011092940.1. NC_004547.2.

Genome annotation databases

EnsemblBacteriaiCAG74265; CAG74265; ECA1355.
KEGGieca:ECA1355.
PATRICifig|218491.5.peg.1386.

Similar proteinsi

Entry informationi

Entry nameiEND8_PECAS
AccessioniPrimary (citable) accession number: Q6D7H0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 92 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families