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Q6D7H0

- END8_PECAS

UniProt

Q6D7H0 - END8_PECAS

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Protein
Endonuclease 8
Gene
nei, ECA1355
Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity.UniRule annotation

Catalytic activityi

Removes damaged bases from DNA, leaving an abasic site.UniRule annotation
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Schiff-base intermediate with DNA By similarity
Active sitei3 – 31Proton donor By similarity
Active sitei53 – 531Proton donor; for beta-elimination activity By similarity
Binding sitei70 – 701DNA By similarity
Binding sitei125 – 1251DNA By similarity
Binding sitei169 – 1691DNA By similarity
Active sitei253 – 2531Proton donor; for delta-elimination activity By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri229 – 26335FPG-typeUniRule annotation
Add
BLAST

GO - Molecular functioni

  1. damaged DNA binding Source: InterPro
  2. oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity Source: UniProtKB-HAMAP
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. base-excision repair Source: InterPro
  2. nucleotide-excision repair Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciPATR218491:GJNB-1392-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 8
Alternative name(s):
DNA glycosylase/AP lyase Nei (EC:3.2.2.-, EC:4.2.99.18)
DNA-(apurinic or apyrimidinic site) lyase Nei
Endonuclease VIII
Gene namesi
Name:nei
Ordered Locus Names:ECA1355
OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Taxonomic identifieri218491 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium
ProteomesiUP000007966: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 263262Endonuclease 8UniRule annotation
PRO_0000170896Add
BLAST

Proteomic databases

PRIDEiQ6D7H0.

Interactioni

Protein-protein interaction databases

STRINGi218491.ECA1355.

Structurei

3D structure databases

ProteinModelPortaliQ6D7H0.
SMRiQ6D7H0. Positions 2-262.

Family & Domainsi

Sequence similaritiesi

Belongs to the FPG family.

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG0266.
HOGENOMiHOG000020882.
KOiK05522.
OMAiGPDVLDM.
OrthoDBiEOG6QP131.

Family and domain databases

HAMAPiMF_01253. Endonuclease_8.
InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR023713. Endonuclease-VIII.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PfamiPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
SMARTiSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEiPS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6D7H0-1 [UniParc]FASTAAdd to Basket

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MPEGPEIRRA ADKLVEAVVG KTLTRVWFAF PELKPYETEL VGQQVRQIET    50
RGKALLTYFS HDRVLYSHNQ LYGVWRVVNA GESPETKRDL RIRLETQDRA 100
ILLYSASDIE MLTLDTLTAH PFLQRIGPDV LDLSLTPEQV CERLLLPRFR 150
RRQFSGLLLD QAFLAGLGNY LRVEILWQAQ LAPQHTASQL NEEQLQTLSR 200
ALLEIPRLSY NTRGTVDENR HHGAIFSFKV FHREGESCER CGGTIERTML 250
SSRPFYWCPH CQS 263
Length:263
Mass (Da):30,336
Last modified:January 23, 2007 - v3
Checksum:iD5117C39CE824EE0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX950851 Genomic DNA. Translation: CAG74265.1.
RefSeqiWP_011092940.1. NC_004547.2.
YP_049461.1. NC_004547.2.

Genome annotation databases

EnsemblBacteriaiCAG74265; CAG74265; ECA1355.
GeneIDi2885656.
KEGGieca:ECA1355.
PATRICi20477982. VBIPecAtr54885_1386.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX950851 Genomic DNA. Translation: CAG74265.1 .
RefSeqi WP_011092940.1. NC_004547.2.
YP_049461.1. NC_004547.2.

3D structure databases

ProteinModelPortali Q6D7H0.
SMRi Q6D7H0. Positions 2-262.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 218491.ECA1355.

Proteomic databases

PRIDEi Q6D7H0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAG74265 ; CAG74265 ; ECA1355 .
GeneIDi 2885656.
KEGGi eca:ECA1355.
PATRICi 20477982. VBIPecAtr54885_1386.

Phylogenomic databases

eggNOGi COG0266.
HOGENOMi HOG000020882.
KOi K05522.
OMAi GPDVLDM.
OrthoDBi EOG6QP131.

Enzyme and pathway databases

BioCyci PATR218491:GJNB-1392-MONOMER.

Family and domain databases

HAMAPi MF_01253. Endonuclease_8.
InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR023713. Endonuclease-VIII.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
Pfami PF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
SMARTi SM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view ]
SUPFAMi SSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEi PS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SCRI 1043 / ATCC BAA-672.

Entry informationi

Entry nameiEND8_PECAS
AccessioniPrimary (citable) accession number: Q6D7H0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 72 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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