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Q6D7H0 (END8_ERWCT) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Endonuclease 8
Alternative name(s):
DNA glycosylase/AP lyase Nei
EC=3.2.2.-
EC=4.2.99.18
DNA-(apurinic or apyrimidinic site) lyase Nei
Endonuclease VIII
Gene names
Name:nei
Ordered Locus Names:ECA1355
OrganismErwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]
Taxonomic identifier29471 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity. HAMAP MF_01253

Catalytic activity

Removes damaged bases from DNA, leaving an abasic site. HAMAP MF_01253

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP MF_01253

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_01253

Sequence similarities

Belongs to the FPG family.

Contains 1 FPG-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 263262Endonuclease 8 HAMAP MF_01253
PRO_0000170896

Regions

Zinc finger229 – 26335FPG-type HAMAP MF_01253

Sites

Active site21Schiff-base intermediate with DNA By similarity
Active site31Proton donor By similarity
Active site531Proton donor; for beta-elimination activity By similarity
Active site2531Proton donor; for delta-elimination activity By similarity
Binding site701DNA By similarity
Binding site1251DNA By similarity
Binding site1691DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6D7H0 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: D5117C39CE824EE0

FASTA26330,336
        10         20         30         40         50         60 
MPEGPEIRRA ADKLVEAVVG KTLTRVWFAF PELKPYETEL VGQQVRQIET RGKALLTYFS 

        70         80         90        100        110        120 
HDRVLYSHNQ LYGVWRVVNA GESPETKRDL RIRLETQDRA ILLYSASDIE MLTLDTLTAH 

       130        140        150        160        170        180 
PFLQRIGPDV LDLSLTPEQV CERLLLPRFR RRQFSGLLLD QAFLAGLGNY LRVEILWQAQ 

       190        200        210        220        230        240 
LAPQHTASQL NEEQLQTLSR ALLEIPRLSY NTRGTVDENR HHGAIFSFKV FHREGESCER 

       250        260 
CGGTIERTML SSRPFYWCPH CQS

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX950851 Genomic DNA. Translation: CAG74265.1.
RefSeqYP_049461.1. NC_004547.2.

3D structure databases

ProteinModelPortalQ6D7H0.
SMRQ6D7H0. Positions 2-262.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2885656.
GenomeReviewsGene locus ECA1355 in contig BX950851_GR.
KEGGeca:ECA1355.
NMPDRfig|218491.3.peg.3546.
PATRIC20477982. VBIPecAtr54885_1386.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG690070.
OMAPRCQPGP.
ProtClustDBPRK10445.

Enzyme and pathway databases

BioCycECAR218491:ECA1355-MONOMER.

Family and domain databases

HAMAPMF_01253. Endonuclease_8.
[Tree]
InterProIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR023713. Endonuclease-VIII.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
KOK05522.
PfamPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
SMARTSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMSSF81624. Form_DNAglyc_cat. 1 hit.
SSF46946. Ribosomal_H2TH. 1 hit.
PROSITEPS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEND8_ERWCT
AccessionPrimary (citable) accession number: Q6D7H0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 23, 2007
Last modified: January 25, 2012
This is version 56 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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