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Q6D736

- BGAL_PECAS

UniProt

Q6D736 - BGAL_PECAS

Protein

Beta-galactosidase

Gene

lacZ

Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei111 – 1111SubstrateUniRule annotation
    Metal bindingi210 – 2101SodiumUniRule annotation
    Binding sitei210 – 2101SubstrateUniRule annotation
    Sitei368 – 3681Transition state stabilizerUniRule annotation
    Sitei402 – 4021Transition state stabilizerUniRule annotation
    Metal bindingi427 – 4271Magnesium 1UniRule annotation
    Metal bindingi429 – 4291Magnesium 1UniRule annotation
    Active sitei472 – 4721Proton donorUniRule annotation
    Metal bindingi472 – 4721Magnesium 1UniRule annotation
    Binding sitei472 – 4721SubstrateUniRule annotation
    Active sitei548 – 5481NucleophileUniRule annotation
    Metal bindingi608 – 6081Magnesium 2UniRule annotation
    Metal bindingi612 – 6121Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi615 – 6151SodiumUniRule annotation
    Binding sitei615 – 6151SubstrateUniRule annotation
    Binding sitei1016 – 10161SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Enzyme and pathway databases

    BioCyciPATR218491:GJNB-1530-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Ordered Locus Names:ECA1490
    OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
    Taxonomic identifieri218491 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium
    ProteomesiUP000007966: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10401040Beta-galactosidasePRO_0000366986Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi218491.ECA1490.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6D736.
    SMRiQ6D736. Positions 22-1035.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni548 – 5514Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000252443.
    KOiK01190.
    OMAiNPPFVPK.
    OrthoDBiEOG6XWV0T.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6D736-1 [UniParc]FASTAAdd to Basket

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    MSDSVLSNPT RQHATLREIL ARRDWENPAC TNYQRLPAHP PFNSWRNVAA     50
    AHQDEPSQRL RRLNGEWKFS YFTRPEAVPE SWLQQDLPDS ATIPVPSNWQ 100
    LQGYDTPIYT NVKYPIPVNP PYVPEDNPTG CYSLTFKVNH DWLSCGQTRV 150
    IFDGVNSAFY LWCNGHWVGY SQDSRLPAEF DISRYLTTGE NRLAVMVLRW 200
    SDGSYLEDQD MWRMSGIFRD VTLLHKPTVH LGDIQLTTPL SADFRHGTLD 250
    IQVKATLSES EAKNYRVHAQ LWRGNNLIGE TRQAFGSDIV DERGTYHDRA 300
    SLRLDVTRPD LWSAELPHLY RAVIALETAE GELLEAEAYD VGFRKVEISN 350
    GLLLLNGKPL LIRGVNRHEH HPQNGQVMDE ETMRRDIMLM KQHNFNAVRC 400
    SHYPNHPLWY RLCDRYGLYV VDEANIETHG MQPMNRLSDD PMWLPAYSER 450
    VSRMVQRDRN HPCIIIWSLG NESGYGANHD ALYQWIKRHD PTRPVHYEGG 500
    GANSRATDIV CPMYARVDED QPFPNVPKWS ISKWISMPNE HRPLILCEYA 550
    HAMGNSLGGF ARYWKAFRQY PRLQGGFIWD WVDQALIRHD EQGNAYWAYG 600
    GDFGDMPNDR QFCLDGLLFP DRTPHPSLYE AQRAQQHIQF VWQAESPCEL 650
    RVTSEYLFRH TDNEQLNWHI TLDDKTLVEG SLPLKLAPQA TQTLTLLESL 700
    PTVDRAGEIW LNVEVVQPKE TAWSKANHRC AWDQWQLPIP LHLPEASCSK 750
    QKIPPVLRAS DIYFDVVQGE QHWRFNRQSG LLEQWWTADT PALLTPLQDQ 800
    FVRAPLDNDI GISEVDRIDP HAWAERWKSA GLYQLQTQCV AIQADQLADA 850
    VHIVTEHVFR HAGQILLRSK KRWQIDAYGV MTVDVDVDVA TVLPSLARVG 900
    LSCQLADVAP QVSWIGLGPH ENYPDRQLAA QHGHWNLPLD DLHTPYIFPS 950
    ENGLRCNTRA LTYGKWAITG NFHFGLSRYG LTQLMTCTHH HLLEKEKGVW 1000
    LNLDGFHMGI GGDDSWSPSV HCDDLLTATH YHYRVAIQRH 1040
    Length:1,040
    Mass (Da):119,655
    Last modified:August 16, 2004 - v1
    Checksum:i040D728F9B080D06
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX950851 Genomic DNA. Translation: CAG74399.1.
    RefSeqiWP_011093073.1. NC_004547.2.
    YP_049595.1. NC_004547.2.

    Genome annotation databases

    EnsemblBacteriaiCAG74399; CAG74399; ECA1490.
    GeneIDi2883666.
    KEGGieca:ECA1490.
    PATRICi20478276. VBIPecAtr54885_1529.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX950851 Genomic DNA. Translation: CAG74399.1 .
    RefSeqi WP_011093073.1. NC_004547.2.
    YP_049595.1. NC_004547.2.

    3D structure databases

    ProteinModelPortali Q6D736.
    SMRi Q6D736. Positions 22-1035.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 218491.ECA1490.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAG74399 ; CAG74399 ; ECA1490 .
    GeneIDi 2883666.
    KEGGi eca:ECA1490.
    PATRICi 20478276. VBIPecAtr54885_1529.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000252443.
    KOi K01190.
    OMAi NPPFVPK.
    OrthoDBi EOG6XWV0T.

    Enzyme and pathway databases

    BioCyci PATR218491:GJNB-1530-MONOMER.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SCRI 1043 / ATCC BAA-672.

    Entry informationi

    Entry nameiBGAL_PECAS
    AccessioniPrimary (citable) accession number: Q6D736
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3