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Protein

Beta-galactosidase

Gene

lacZ

Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na+UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei111SubstrateUniRule annotation1
Metal bindingi210SodiumUniRule annotation1
Binding sitei210SubstrateUniRule annotation1
Sitei368Transition state stabilizerUniRule annotation1
Sitei402Transition state stabilizerUniRule annotation1
Metal bindingi427Magnesium 1UniRule annotation1
Metal bindingi429Magnesium 1UniRule annotation1
Active sitei472Proton donorUniRule annotation1
Metal bindingi472Magnesium 1UniRule annotation1
Binding sitei472SubstrateUniRule annotation1
Active sitei548NucleophileUniRule annotation1
Metal bindingi608Magnesium 2UniRule annotation1
Metal bindingi612Sodium; via carbonyl oxygenUniRule annotation1
Metal bindingi615SodiumUniRule annotation1
Binding sitei615SubstrateUniRule annotation1
Binding sitei1016SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:ECA1490
OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Taxonomic identifieri218491 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaePectobacterium
Proteomesi
  • UP000007966 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003669861 – 1040Beta-galactosidaseAdd BLAST1040

Proteomic databases

PRIDEiQ6D736.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi218491.ECA1490.

Structurei

3D structure databases

ProteinModelPortaliQ6D736.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni548 – 551Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000252443.
KOiK01190.
OMAiCLDGLLF.
OrthoDBiPOG091H07QB.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6D736-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDSVLSNPT RQHATLREIL ARRDWENPAC TNYQRLPAHP PFNSWRNVAA
60 70 80 90 100
AHQDEPSQRL RRLNGEWKFS YFTRPEAVPE SWLQQDLPDS ATIPVPSNWQ
110 120 130 140 150
LQGYDTPIYT NVKYPIPVNP PYVPEDNPTG CYSLTFKVNH DWLSCGQTRV
160 170 180 190 200
IFDGVNSAFY LWCNGHWVGY SQDSRLPAEF DISRYLTTGE NRLAVMVLRW
210 220 230 240 250
SDGSYLEDQD MWRMSGIFRD VTLLHKPTVH LGDIQLTTPL SADFRHGTLD
260 270 280 290 300
IQVKATLSES EAKNYRVHAQ LWRGNNLIGE TRQAFGSDIV DERGTYHDRA
310 320 330 340 350
SLRLDVTRPD LWSAELPHLY RAVIALETAE GELLEAEAYD VGFRKVEISN
360 370 380 390 400
GLLLLNGKPL LIRGVNRHEH HPQNGQVMDE ETMRRDIMLM KQHNFNAVRC
410 420 430 440 450
SHYPNHPLWY RLCDRYGLYV VDEANIETHG MQPMNRLSDD PMWLPAYSER
460 470 480 490 500
VSRMVQRDRN HPCIIIWSLG NESGYGANHD ALYQWIKRHD PTRPVHYEGG
510 520 530 540 550
GANSRATDIV CPMYARVDED QPFPNVPKWS ISKWISMPNE HRPLILCEYA
560 570 580 590 600
HAMGNSLGGF ARYWKAFRQY PRLQGGFIWD WVDQALIRHD EQGNAYWAYG
610 620 630 640 650
GDFGDMPNDR QFCLDGLLFP DRTPHPSLYE AQRAQQHIQF VWQAESPCEL
660 670 680 690 700
RVTSEYLFRH TDNEQLNWHI TLDDKTLVEG SLPLKLAPQA TQTLTLLESL
710 720 730 740 750
PTVDRAGEIW LNVEVVQPKE TAWSKANHRC AWDQWQLPIP LHLPEASCSK
760 770 780 790 800
QKIPPVLRAS DIYFDVVQGE QHWRFNRQSG LLEQWWTADT PALLTPLQDQ
810 820 830 840 850
FVRAPLDNDI GISEVDRIDP HAWAERWKSA GLYQLQTQCV AIQADQLADA
860 870 880 890 900
VHIVTEHVFR HAGQILLRSK KRWQIDAYGV MTVDVDVDVA TVLPSLARVG
910 920 930 940 950
LSCQLADVAP QVSWIGLGPH ENYPDRQLAA QHGHWNLPLD DLHTPYIFPS
960 970 980 990 1000
ENGLRCNTRA LTYGKWAITG NFHFGLSRYG LTQLMTCTHH HLLEKEKGVW
1010 1020 1030 1040
LNLDGFHMGI GGDDSWSPSV HCDDLLTATH YHYRVAIQRH
Length:1,040
Mass (Da):119,655
Last modified:August 16, 2004 - v1
Checksum:i040D728F9B080D06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950851 Genomic DNA. Translation: CAG74399.1.
RefSeqiWP_011093073.1. NC_004547.2.

Genome annotation databases

EnsemblBacteriaiCAG74399; CAG74399; ECA1490.
GeneIDi2883666.
KEGGieca:ECA1490.
PATRICi20478276. VBIPecAtr54885_1529.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950851 Genomic DNA. Translation: CAG74399.1.
RefSeqiWP_011093073.1. NC_004547.2.

3D structure databases

ProteinModelPortaliQ6D736.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi218491.ECA1490.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Proteomic databases

PRIDEiQ6D736.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG74399; CAG74399; ECA1490.
GeneIDi2883666.
KEGGieca:ECA1490.
PATRICi20478276. VBIPecAtr54885_1529.

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000252443.
KOiK01190.
OMAiCLDGLLF.
OrthoDBiPOG091H07QB.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGAL_PECAS
AccessioniPrimary (citable) accession number: Q6D736
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: August 16, 2004
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.