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Q6D736 (BGAL_PECAS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
Ordered Locus Names:ECA1490
OrganismPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica) [Complete proteome] [HAMAP]
Taxonomic identifier218491 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium

Protein attributes

Sequence length1040 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP-Rule MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity. HAMAP-Rule MF_01687

Binds 1 sodium ion per monomer By similarity. HAMAP-Rule MF_01687

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10401040Beta-galactosidase HAMAP-Rule MF_01687
PRO_0000366986

Regions

Region548 – 5514Substrate binding By similarity

Sites

Active site4721Proton donor By similarity
Active site5481Nucleophile By similarity
Metal binding2101Sodium By similarity
Metal binding4271Magnesium 1 By similarity
Metal binding4291Magnesium 1 By similarity
Metal binding4721Magnesium 1 By similarity
Metal binding6081Magnesium 2 By similarity
Metal binding6121Sodium; via carbonyl oxygen By similarity
Metal binding6151Sodium By similarity
Binding site1111Substrate By similarity
Binding site2101Substrate By similarity
Binding site4721Substrate By similarity
Binding site6151Substrate By similarity
Binding site10161Substrate By similarity
Site3681Transition state stabilizer By similarity
Site4021Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6D736 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 040D728F9B080D06

FASTA1,040119,655
        10         20         30         40         50         60 
MSDSVLSNPT RQHATLREIL ARRDWENPAC TNYQRLPAHP PFNSWRNVAA AHQDEPSQRL 

        70         80         90        100        110        120 
RRLNGEWKFS YFTRPEAVPE SWLQQDLPDS ATIPVPSNWQ LQGYDTPIYT NVKYPIPVNP 

       130        140        150        160        170        180 
PYVPEDNPTG CYSLTFKVNH DWLSCGQTRV IFDGVNSAFY LWCNGHWVGY SQDSRLPAEF 

       190        200        210        220        230        240 
DISRYLTTGE NRLAVMVLRW SDGSYLEDQD MWRMSGIFRD VTLLHKPTVH LGDIQLTTPL 

       250        260        270        280        290        300 
SADFRHGTLD IQVKATLSES EAKNYRVHAQ LWRGNNLIGE TRQAFGSDIV DERGTYHDRA 

       310        320        330        340        350        360 
SLRLDVTRPD LWSAELPHLY RAVIALETAE GELLEAEAYD VGFRKVEISN GLLLLNGKPL 

       370        380        390        400        410        420 
LIRGVNRHEH HPQNGQVMDE ETMRRDIMLM KQHNFNAVRC SHYPNHPLWY RLCDRYGLYV 

       430        440        450        460        470        480 
VDEANIETHG MQPMNRLSDD PMWLPAYSER VSRMVQRDRN HPCIIIWSLG NESGYGANHD 

       490        500        510        520        530        540 
ALYQWIKRHD PTRPVHYEGG GANSRATDIV CPMYARVDED QPFPNVPKWS ISKWISMPNE 

       550        560        570        580        590        600 
HRPLILCEYA HAMGNSLGGF ARYWKAFRQY PRLQGGFIWD WVDQALIRHD EQGNAYWAYG 

       610        620        630        640        650        660 
GDFGDMPNDR QFCLDGLLFP DRTPHPSLYE AQRAQQHIQF VWQAESPCEL RVTSEYLFRH 

       670        680        690        700        710        720 
TDNEQLNWHI TLDDKTLVEG SLPLKLAPQA TQTLTLLESL PTVDRAGEIW LNVEVVQPKE 

       730        740        750        760        770        780 
TAWSKANHRC AWDQWQLPIP LHLPEASCSK QKIPPVLRAS DIYFDVVQGE QHWRFNRQSG 

       790        800        810        820        830        840 
LLEQWWTADT PALLTPLQDQ FVRAPLDNDI GISEVDRIDP HAWAERWKSA GLYQLQTQCV 

       850        860        870        880        890        900 
AIQADQLADA VHIVTEHVFR HAGQILLRSK KRWQIDAYGV MTVDVDVDVA TVLPSLARVG 

       910        920        930        940        950        960 
LSCQLADVAP QVSWIGLGPH ENYPDRQLAA QHGHWNLPLD DLHTPYIFPS ENGLRCNTRA 

       970        980        990       1000       1010       1020 
LTYGKWAITG NFHFGLSRYG LTQLMTCTHH HLLEKEKGVW LNLDGFHMGI GGDDSWSPSV 

      1030       1040 
HCDDLLTATH YHYRVAIQRH 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX950851 Genomic DNA. Translation: CAG74399.1.
RefSeqYP_049595.1. NC_004547.2.

3D structure databases

ProteinModelPortalQ6D736.
SMRQ6D736. Positions 22-1035.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING218491.ECA1490.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAG74399; CAG74399; ECA1490.
GeneID2883666.
KEGGeca:ECA1490.
PATRIC20478276. VBIPecAtr54885_1529.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHOG000252443.
KOK01190.
OMANPPFVPK.
OrthoDBEOG6XWV0T.

Enzyme and pathway databases

BioCycPATR218491:GJNB-1530-MONOMER.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_01687. Beta_gal.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_PECAS
AccessionPrimary (citable) accession number: Q6D736
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: August 16, 2004
Last modified: May 14, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries