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Q6D736

- BGAL_PECAS

UniProt

Q6D736 - BGAL_PECAS

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Protein

Beta-galactosidase

Gene

lacZ

Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer.UniRule annotation
Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei111 – 1111SubstrateUniRule annotation
Metal bindingi210 – 2101SodiumUniRule annotation
Binding sitei210 – 2101SubstrateUniRule annotation
Sitei368 – 3681Transition state stabilizerUniRule annotation
Sitei402 – 4021Transition state stabilizerUniRule annotation
Metal bindingi427 – 4271Magnesium 1UniRule annotation
Metal bindingi429 – 4291Magnesium 1UniRule annotation
Active sitei472 – 4721Proton donorUniRule annotation
Metal bindingi472 – 4721Magnesium 1UniRule annotation
Binding sitei472 – 4721SubstrateUniRule annotation
Active sitei548 – 5481NucleophileUniRule annotation
Metal bindingi608 – 6081Magnesium 2UniRule annotation
Metal bindingi612 – 6121Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi615 – 6151SodiumUniRule annotation
Binding sitei615 – 6151SubstrateUniRule annotation
Binding sitei1016 – 10161SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciPATR218491:GJNB-1530-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:ECA1490
OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Taxonomic identifieri218491 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium
ProteomesiUP000007966: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10401040Beta-galactosidasePRO_0000366986Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi218491.ECA1490.

Structurei

3D structure databases

ProteinModelPortaliQ6D736.
SMRiQ6D736. Positions 22-1035.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni548 – 5514Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiNPPFVPK.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6D736 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDSVLSNPT RQHATLREIL ARRDWENPAC TNYQRLPAHP PFNSWRNVAA
60 70 80 90 100
AHQDEPSQRL RRLNGEWKFS YFTRPEAVPE SWLQQDLPDS ATIPVPSNWQ
110 120 130 140 150
LQGYDTPIYT NVKYPIPVNP PYVPEDNPTG CYSLTFKVNH DWLSCGQTRV
160 170 180 190 200
IFDGVNSAFY LWCNGHWVGY SQDSRLPAEF DISRYLTTGE NRLAVMVLRW
210 220 230 240 250
SDGSYLEDQD MWRMSGIFRD VTLLHKPTVH LGDIQLTTPL SADFRHGTLD
260 270 280 290 300
IQVKATLSES EAKNYRVHAQ LWRGNNLIGE TRQAFGSDIV DERGTYHDRA
310 320 330 340 350
SLRLDVTRPD LWSAELPHLY RAVIALETAE GELLEAEAYD VGFRKVEISN
360 370 380 390 400
GLLLLNGKPL LIRGVNRHEH HPQNGQVMDE ETMRRDIMLM KQHNFNAVRC
410 420 430 440 450
SHYPNHPLWY RLCDRYGLYV VDEANIETHG MQPMNRLSDD PMWLPAYSER
460 470 480 490 500
VSRMVQRDRN HPCIIIWSLG NESGYGANHD ALYQWIKRHD PTRPVHYEGG
510 520 530 540 550
GANSRATDIV CPMYARVDED QPFPNVPKWS ISKWISMPNE HRPLILCEYA
560 570 580 590 600
HAMGNSLGGF ARYWKAFRQY PRLQGGFIWD WVDQALIRHD EQGNAYWAYG
610 620 630 640 650
GDFGDMPNDR QFCLDGLLFP DRTPHPSLYE AQRAQQHIQF VWQAESPCEL
660 670 680 690 700
RVTSEYLFRH TDNEQLNWHI TLDDKTLVEG SLPLKLAPQA TQTLTLLESL
710 720 730 740 750
PTVDRAGEIW LNVEVVQPKE TAWSKANHRC AWDQWQLPIP LHLPEASCSK
760 770 780 790 800
QKIPPVLRAS DIYFDVVQGE QHWRFNRQSG LLEQWWTADT PALLTPLQDQ
810 820 830 840 850
FVRAPLDNDI GISEVDRIDP HAWAERWKSA GLYQLQTQCV AIQADQLADA
860 870 880 890 900
VHIVTEHVFR HAGQILLRSK KRWQIDAYGV MTVDVDVDVA TVLPSLARVG
910 920 930 940 950
LSCQLADVAP QVSWIGLGPH ENYPDRQLAA QHGHWNLPLD DLHTPYIFPS
960 970 980 990 1000
ENGLRCNTRA LTYGKWAITG NFHFGLSRYG LTQLMTCTHH HLLEKEKGVW
1010 1020 1030 1040
LNLDGFHMGI GGDDSWSPSV HCDDLLTATH YHYRVAIQRH
Length:1,040
Mass (Da):119,655
Last modified:August 16, 2004 - v1
Checksum:i040D728F9B080D06
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX950851 Genomic DNA. Translation: CAG74399.1.
RefSeqiYP_049595.1. NC_004547.2.

Genome annotation databases

EnsemblBacteriaiCAG74399; CAG74399; ECA1490.
GeneIDi2883666.
KEGGieca:ECA1490.
PATRICi20478276. VBIPecAtr54885_1529.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX950851 Genomic DNA. Translation: CAG74399.1 .
RefSeqi YP_049595.1. NC_004547.2.

3D structure databases

ProteinModelPortali Q6D736.
SMRi Q6D736. Positions 22-1035.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 218491.ECA1490.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAG74399 ; CAG74399 ; ECA1490 .
GeneIDi 2883666.
KEGGi eca:ECA1490.
PATRICi 20478276. VBIPecAtr54885_1529.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi NPPFVPK.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci PATR218491:GJNB-1530-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SCRI 1043 / ATCC BAA-672.

Entry informationi

Entry nameiBGAL_PECAS
AccessioniPrimary (citable) accession number: Q6D736
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: August 16, 2004
Last modified: October 29, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3