Reviewed,
UniProtKB/Swiss-Prot Q6D736 (BGAL_ERWCT)
Last modified
January 19, 2010.
Version 36.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Beta-galactosidase Short name=Beta-gal EC=3.2.1.23 Alternative name(s): Lactase | ||||
| Gene names |
| ||||
| Organism | Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 29471 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium |
Protein attributes
| Sequence length | 1040 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Catalytic activity | Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP MF_01687 |
| Cofactor | Binds 2 magnesium ions per monomer By similarity. HAMAP MF_01687 Binds 1 sodium ion per monomer By similarity. HAMAP MF_01687 |
| Subunit structure | Homotetramer By similarity. HAMAP MF_01687 |
| Sequence similarities | Belongs to the glycosyl hydrolase 2 family. |
Ontologies
| Keywords | |
|---|---|
| Ligand | Magnesium Metal-binding Sodium |
| Molecular function | Glycosidase Hydrolase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro |
| Cellular component | beta-galactosidase complex Inferred from electronic annotation. Source: InterPro |
| Molecular function | beta-galactosidase activity Inferred from electronic annotation. Source: EC carbohydrate bindingInferred from electronic annotation. Source: InterPro magnesium ion bindingInferred from electronic annotation. Source: HAMAP sodium ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1040 | 1040 | Beta-galactosidase HAMAP MF_01687 | PRO_0000366986 | |||||
Regions | |||||||||
| Region | 548 – 551 | 4 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Active site | 472 | 1 | Proton donor By similarity | ||||||
| Active site | 548 | 1 | Nucleophile By similarity | ||||||
| Metal binding | 210 | 1 | Sodium By similarity | ||||||
| Metal binding | 427 | 1 | Magnesium 1 By similarity | ||||||
| Metal binding | 429 | 1 | Magnesium 1 By similarity | ||||||
| Metal binding | 472 | 1 | Magnesium 1 By similarity | ||||||
| Metal binding | 608 | 1 | Magnesium 2 By similarity | ||||||
| Metal binding | 612 | 1 | Sodium; via carbonyl oxygen By similarity | ||||||
| Metal binding | 615 | 1 | Sodium By similarity | ||||||
| Binding site | 111 | 1 | Substrate By similarity | ||||||
| Binding site | 210 | 1 | Substrate By similarity | ||||||
| Binding site | 472 | 1 | Substrate By similarity | ||||||
| Binding site | 615 | 1 | Substrate By similarity | ||||||
| Binding site | 1016 | 1 | Substrate By similarity | ||||||
| Site | 368 | 1 | Transition state stabilizer By similarity | ||||||
| Site | 402 | 1 | Transition state stabilizer By similarity | ||||||
Sequences
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References
| [1] | "Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. Toth I.K.Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BX950851 Genomic DNA. Translation: CAG74399.1. |
| RefSeq | YP_049595.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1PX3 based on UniProtKB P00722. |
| SMR | Q6D736. Positions 22-1035. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 2883666. |
| GenomeReviews | Gene locus ECA1490 in contig BX950851_GR. |
| KEGG | eca:ECA1490. |
| NMPDR | fig|218491.3.peg.3949. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | HBG670643. |
| OMA | LDACYRD. |
Enzyme and pathway databases | |
| BioCyc | ECAR218491:ECA1490-MONOMER. |
Family and domain databases | |
| HAMAP | MF_01687. Beta_gal. [Tree] |
| InterPro | IPR008979. Galactose-bd-like. IPR011013. Glyco_hydro-type_carb-bd. IPR014718. Glyco_hydro-type_carb-bd_sub. IPR006101. Glyco_hydro_2. IPR013812. Glyco_hydro_2/20_Ig-like. IPR006104. Glyco_hydro_2_carb-bd. IPR006102. Glyco_hydro_2_Ig-like. IPR006103. Glyco_hydro_2_TIM. IPR004199. Glyco_hydro_42_D5. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view] |
| Gene3D | G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 2 hits. G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. |
| Pfam | PF02929. Bgal_small_N. 1 hit. PF00703. Glyco_hydro_2. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view] |
| PRINTS | PR00132. GLHYDRLASE2. |
| PROSITE | PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | BGAL_ERWCT | ||||||||
| Accession | Primary (citable) accession number: Q6D736 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


