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Q6D736

- BGAL_PECAS

UniProt

Q6D736 - BGAL_PECAS

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Protein

Beta-galactosidase

Gene
lacZ, ECA1490
Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer By similarity.UniRule annotation
Binds 1 sodium ion per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei111 – 1111Substrate By similarity
Metal bindingi210 – 2101Sodium By similarity
Binding sitei210 – 2101Substrate By similarity
Sitei368 – 3681Transition state stabilizer By similarity
Sitei402 – 4021Transition state stabilizer By similarity
Metal bindingi427 – 4271Magnesium 1 By similarity
Metal bindingi429 – 4291Magnesium 1 By similarity
Active sitei472 – 4721Proton donor By similarity
Metal bindingi472 – 4721Magnesium 1 By similarity
Binding sitei472 – 4721Substrate By similarity
Active sitei548 – 5481Nucleophile By similarity
Metal bindingi608 – 6081Magnesium 2 By similarity
Metal bindingi612 – 6121Sodium; via carbonyl oxygen By similarity
Metal bindingi615 – 6151Sodium By similarity
Binding sitei615 – 6151Substrate By similarity
Binding sitei1016 – 10161Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciPATR218491:GJNB-1530-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
Ordered Locus Names:ECA1490
OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Taxonomic identifieri218491 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium
ProteomesiUP000007966: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10401040Beta-galactosidaseUniRule annotationPRO_0000366986Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi218491.ECA1490.

Structurei

3D structure databases

ProteinModelPortaliQ6D736.
SMRiQ6D736. Positions 22-1035.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni548 – 5514Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiNPPFVPK.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6D736-1 [UniParc]FASTAAdd to Basket

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MSDSVLSNPT RQHATLREIL ARRDWENPAC TNYQRLPAHP PFNSWRNVAA     50
AHQDEPSQRL RRLNGEWKFS YFTRPEAVPE SWLQQDLPDS ATIPVPSNWQ 100
LQGYDTPIYT NVKYPIPVNP PYVPEDNPTG CYSLTFKVNH DWLSCGQTRV 150
IFDGVNSAFY LWCNGHWVGY SQDSRLPAEF DISRYLTTGE NRLAVMVLRW 200
SDGSYLEDQD MWRMSGIFRD VTLLHKPTVH LGDIQLTTPL SADFRHGTLD 250
IQVKATLSES EAKNYRVHAQ LWRGNNLIGE TRQAFGSDIV DERGTYHDRA 300
SLRLDVTRPD LWSAELPHLY RAVIALETAE GELLEAEAYD VGFRKVEISN 350
GLLLLNGKPL LIRGVNRHEH HPQNGQVMDE ETMRRDIMLM KQHNFNAVRC 400
SHYPNHPLWY RLCDRYGLYV VDEANIETHG MQPMNRLSDD PMWLPAYSER 450
VSRMVQRDRN HPCIIIWSLG NESGYGANHD ALYQWIKRHD PTRPVHYEGG 500
GANSRATDIV CPMYARVDED QPFPNVPKWS ISKWISMPNE HRPLILCEYA 550
HAMGNSLGGF ARYWKAFRQY PRLQGGFIWD WVDQALIRHD EQGNAYWAYG 600
GDFGDMPNDR QFCLDGLLFP DRTPHPSLYE AQRAQQHIQF VWQAESPCEL 650
RVTSEYLFRH TDNEQLNWHI TLDDKTLVEG SLPLKLAPQA TQTLTLLESL 700
PTVDRAGEIW LNVEVVQPKE TAWSKANHRC AWDQWQLPIP LHLPEASCSK 750
QKIPPVLRAS DIYFDVVQGE QHWRFNRQSG LLEQWWTADT PALLTPLQDQ 800
FVRAPLDNDI GISEVDRIDP HAWAERWKSA GLYQLQTQCV AIQADQLADA 850
VHIVTEHVFR HAGQILLRSK KRWQIDAYGV MTVDVDVDVA TVLPSLARVG 900
LSCQLADVAP QVSWIGLGPH ENYPDRQLAA QHGHWNLPLD DLHTPYIFPS 950
ENGLRCNTRA LTYGKWAITG NFHFGLSRYG LTQLMTCTHH HLLEKEKGVW 1000
LNLDGFHMGI GGDDSWSPSV HCDDLLTATH YHYRVAIQRH 1040
Length:1,040
Mass (Da):119,655
Last modified:August 16, 2004 - v1
Checksum:i040D728F9B080D06
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX950851 Genomic DNA. Translation: CAG74399.1.
RefSeqiWP_011093073.1. NC_004547.2.
YP_049595.1. NC_004547.2.

Genome annotation databases

EnsemblBacteriaiCAG74399; CAG74399; ECA1490.
GeneIDi2883666.
KEGGieca:ECA1490.
PATRICi20478276. VBIPecAtr54885_1529.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX950851 Genomic DNA. Translation: CAG74399.1 .
RefSeqi WP_011093073.1. NC_004547.2.
YP_049595.1. NC_004547.2.

3D structure databases

ProteinModelPortali Q6D736.
SMRi Q6D736. Positions 22-1035.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 218491.ECA1490.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAG74399 ; CAG74399 ; ECA1490 .
GeneIDi 2883666.
KEGGi eca:ECA1490.
PATRICi 20478276. VBIPecAtr54885_1529.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi NPPFVPK.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci PATR218491:GJNB-1530-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SCRI 1043 / ATCC BAA-672.

Entry informationi

Entry nameiBGAL_PECAS
AccessioniPrimary (citable) accession number: Q6D736
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: August 16, 2004
Last modified: September 3, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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