Reviewed, UniProtKB/Swiss-Prot Q6D736 (BGAL_ERWCT)

Names and origin

Protein names

Recommended name:
    Beta-galactosidase
      Short name=Beta-gal
    EC=3.2.1.23
Alternative name(s):
    Lactase

Gene names

Name:	lacZ
Ordered Locus Names:	ECA1490

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	1040 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP MF_01687
Cofactor	Binds 2 magnesium ions per monomer By similarity. HAMAP MF_01687 Binds 1 sodium ion per monomer By similarity. HAMAP MF_01687
Subunit structure	Homotetramer By similarity. HAMAP MF_01687
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding Sodium
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	beta-galactosidase complex Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: HAMAP sodium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1040	1040	Beta-galactosidase HAMAP MF_01687		PRO_0000366986
Regions
	Region	548 – 551	4	Substrate binding By similarity
Sites
	Active site	472	1	Proton donor By similarity
	Active site	548	1	Nucleophile By similarity
	Metal binding	210	1	Sodium By similarity
	Metal binding	427	1	Magnesium 1 By similarity
	Metal binding	429	1	Magnesium 1 By similarity
	Metal binding	472	1	Magnesium 1 By similarity
	Metal binding	608	1	Magnesium 2 By similarity
	Metal binding	612	1	Sodium; via carbonyl oxygen By similarity
	Metal binding	615	1	Sodium By similarity
	Binding site	111	1	Substrate By similarity
	Binding site	210	1	Substrate By similarity
	Binding site	472	1	Substrate By similarity
	Binding site	615	1	Substrate By similarity
	Binding site	1016	1	Substrate By similarity
	Site	368	1	Transition state stabilizer By similarity
	Site	402	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6D736-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 040D728F9B080D06	FASTA	1,040	119,655
	10 20 30 40 50 60 MSDSVLSNPT RQHATLREIL ARRDWENPAC TNYQRLPAHP PFNSWRNVAA AHQDEPSQRL 70 80 90 100 110 120 RRLNGEWKFS YFTRPEAVPE SWLQQDLPDS ATIPVPSNWQ LQGYDTPIYT NVKYPIPVNP 130 140 150 160 170 180 PYVPEDNPTG CYSLTFKVNH DWLSCGQTRV IFDGVNSAFY LWCNGHWVGY SQDSRLPAEF 190 200 210 220 230 240 DISRYLTTGE NRLAVMVLRW SDGSYLEDQD MWRMSGIFRD VTLLHKPTVH LGDIQLTTPL 250 260 270 280 290 300 SADFRHGTLD IQVKATLSES EAKNYRVHAQ LWRGNNLIGE TRQAFGSDIV DERGTYHDRA 310 320 330 340 350 360 SLRLDVTRPD LWSAELPHLY RAVIALETAE GELLEAEAYD VGFRKVEISN GLLLLNGKPL 370 380 390 400 410 420 LIRGVNRHEH HPQNGQVMDE ETMRRDIMLM KQHNFNAVRC SHYPNHPLWY RLCDRYGLYV 430 440 450 460 470 480 VDEANIETHG MQPMNRLSDD PMWLPAYSER VSRMVQRDRN HPCIIIWSLG NESGYGANHD 490 500 510 520 530 540 ALYQWIKRHD PTRPVHYEGG GANSRATDIV CPMYARVDED QPFPNVPKWS ISKWISMPNE 550 560 570 580 590 600 HRPLILCEYA HAMGNSLGGF ARYWKAFRQY PRLQGGFIWD WVDQALIRHD EQGNAYWAYG 610 620 630 640 650 660 GDFGDMPNDR QFCLDGLLFP DRTPHPSLYE AQRAQQHIQF VWQAESPCEL RVTSEYLFRH 670 680 690 700 710 720 TDNEQLNWHI TLDDKTLVEG SLPLKLAPQA TQTLTLLESL PTVDRAGEIW LNVEVVQPKE 730 740 750 760 770 780 TAWSKANHRC AWDQWQLPIP LHLPEASCSK QKIPPVLRAS DIYFDVVQGE QHWRFNRQSG 790 800 810 820 830 840 LLEQWWTADT PALLTPLQDQ FVRAPLDNDI GISEVDRIDP HAWAERWKSA GLYQLQTQCV 850 860 870 880 890 900 AIQADQLADA VHIVTEHVFR HAGQILLRSK KRWQIDAYGV MTVDVDVDVA TVLPSLARVG 910 920 930 940 950 960 LSCQLADVAP QVSWIGLGPH ENYPDRQLAA QHGHWNLPLD DLHTPYIFPS ENGLRCNTRA 970 980 990 1000 1010 1020 LTYGKWAITG NFHFGLSRYG LTQLMTCTHH HLLEKEKGVW LNLDGFHMGI GGDDSWSPSV 1030 1040 HCDDLLTATH YHYRVAIQRH

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950851 Genomic DNA. Translation: CAG74399.1.
RefSeq	YP_049595.1.
3D structure databases
HSSP	HSSP built from PDB template 1PX3 based on UniProtKB P00722.
SMR	Q6D736. Positions 22-1035.
ModBase	Search...
Genome annotation databases
GeneID	2883666.
GenomeReviews	Gene locus ECA1490 in contig BX950851_GR.
KEGG	eca:ECA1490.
NMPDR	fig|218491.3.peg.3949.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG670643.
OMA	LDACYRD.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA1490-MONOMER.
Family and domain databases
HAMAP	MF_01687. Beta_gal. [Tree]
InterPro	IPR008979. Galactose-bd-like. IPR011013. Glyco_hydro-type_carb-bd. IPR014718. Glyco_hydro-type_carb-bd_sub. IPR006101. Glyco_hydro_2. IPR013812. Glyco_hydro_2/20_Ig-like. IPR006104. Glyco_hydro_2_carb-bd. IPR006102. Glyco_hydro_2_Ig-like. IPR006103. Glyco_hydro_2_TIM. IPR004199. Glyco_hydro_42_D5. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 2 hits. G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF02929. Bgal_small_N. 1 hit. PF00703. Glyco_hydro_2. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
PRINTS	PR00132. GLHYDRLASE2.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_ERWCT

Accession

Primary (citable) accession number: Q6D736

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	August 16, 2004
Last modified:	January 19, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)