Reviewed, UniProtKB/Swiss-Prot Q6D6D9 (BETA_ERWCT)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	ECA1746

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity. HAMAP MF_00750
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity. HAMAP MF_00750
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 559	559	Choline dehydrogenase HAMAP MF_00750		PRO_0000258924
Regions
	Nucleotide binding	4 – 33	30	FAD Probable
Sites
	Active site	471	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6D6D9-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: E7BD0CA73F8D314A	FASTA	559	62,102
	10 20 30 40 50 60 MEYDYIIIGA GSAGNVLAAR LTEESDVSVL LLEAGGPDYR LDFRTQMPAA LAFPLQGKRY 70 80 90 100 110 120 NWAYETDPEP HMNDRRMECG RGKGLGGSSL INGMCYIRGN AMDFDHWATM SGLEDWSYLD 130 140 150 160 170 180 CLPYFRKAET RDVGANDYHG ASGPVSVTTP KMGNNELFHA MVEAGVQAGY PRTDDLNGYQ 190 200 210 220 230 240 QEGFGPMDRT VTPKGRRAST ARGYLDQAKS RKNLTIVTHA LTDRILFDGK RAVGVAYFKG 250 260 270 280 290 300 ESAQTAKARR EVLLCAGAIA SPQILQRSGV GPKDLLQRLD ISVVHDLPGV GENLQDHLEM 310 320 330 340 350 360 YLQYACKEPV SLYPALQWFN QPKIGAEWLF NGTGIGASNQ FEAGGFIRSR DEFTWPNIQY 370 380 390 400 410 420 HFLPVAINYN GSNAVKEHGF QAHVGSMRSL SRGRVQVRSK DAREHPSILF NYMSTEQDWQ 430 440 450 460 470 480 EFRDAIRITR EIMAQPALDK YRGREISPGL AVQTDEELDE FIRTHAETAF HPSCSCKMGE 490 500 510 520 530 540 DDMSVVDGQG CVHGMEGLRV VDASIMPQII TGNLNATTIM IAEKIADRIR RRQPLPRSKA 550 RYYVAGSTPV RKAPLKPAS

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950851 Genomic DNA. Translation: CAG74651.1.
RefSeq	YP_049846.1.
3D structure databases
SMR	Q6D6D9. Positions 2-529.
ModBase	Search...
Genome annotation databases
GeneID	2882565.
GenomeReviews	Gene locus ECA1746 in contig BX950851_GR.
KEGG	eca:ECA1746.
NMPDR	fig|218491.3.peg.216.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734713.
OMA	RTVTPHG.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA1746-MONOMER.
BRENDA	1.1.99.1. 281579.
Family and domain databases
HAMAP	MF_00750. Choline_dehydrogen. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_ERWCT

Accession

Primary (citable) accession number: Q6D6D9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	August 16, 2004
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)