Dihydroorotase - Erwinia carotovora subsp. atroseptica

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Reviewed, UniProtKB/Swiss-Prot Q6D698 (PYRC_ERWCT)

Last modified November 25, 2008. Version 32. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	ECA1787

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

347

Dihydroorotase

PRO_1000024013

Sites

Metal binding

1

Zinc 1 By similarity

Metal binding

1

Zinc 1 By similarity

Metal binding

1

Zinc 1; via carbamate group By similarity

Metal binding

1

Zinc 2; via carbamate group By similarity

Metal binding

1

Zinc 2 By similarity

Metal binding

1

Zinc 2 By similarity

Metal binding

1

Zinc 1 By similarity

Amino acid modifications

Modified residue

1

N6-carboxylysine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q6D698-1 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 15507D1F7ABD903E
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347

38,615

        10         20         30         40         50         60 
MTAQHTILKI RRPDDWHIHL RDDRMLETVL PYTSRFFGRA IVMPNLTPPI TSVASAIAYR 

        70         80         90        100        110        120 
QRILAAVPQG DNFHPLMTCY LTDTLDANEI VSGFEQGVFT AAKLYPANAT TNSSHGVTSV 

       130        140        150        160        170        180 
ATISGILEKM QKIGMPLLIH GEVTDPAIDI FDREARFIET ILEPLRQDFP ELKVVLEHIT 

       190        200        210        220        230        240 
TKEAAEYVIA GNDYLAATIT PQHLMFNRNH MLVGGIRTHL YCLPILKRNT HQQALREAVA 

       250        260        270        280        290        300 
SGCDRLFLGT DSAPHAKHRK ESSCGCAGVF NAQAALSTYA TVFEEMNALD KLEAFCSLNG 

       310        320        330        340 
PRFYGLPVND SWIELHREAV TFPEEIALGD ESLIPFLAGQ SLNWSVR

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors."
Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z.

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Toth I.K.
Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
	BX950851 Genomic DNA. Translation: CAG74692.1.
RefSeq	YP_049887.1.
3D structure databases
SMR	Q6D698. Positions 7-347.
ModBase	Search...
Genome annotation databases
GeneID	2882669.
GenomeReviews	Gene locus ECA1787 in contig BX950851_GR.
KEGG	eca:ECA1787.
NMPDR	fig\|218491.3.peg.257.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6D698.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA1787-MON.
Family and domain databases
HAMAP	MF_00219. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. pyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. False negative. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_ERWCT

Accession

Primary (citable) accession number: Q6D698

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 16, 2004
Last modified:	November 25, 2008
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents