Reviewed, UniProtKB/Swiss-Prot Q6D674 (NAGZ_ERWCT)

Names and origin

Protein names

Recommended name:
    Beta-hexosaminidase
    EC=3.2.1.52
Alternative name(s):
    N-acetyl-beta-glucosaminidase
    Beta-N-acetylhexosaminidase

Gene names

Name:	nagZ
Ordered Locus Names:	ECA1813

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves GlcNAc linked beta-1,4 to MurNAc tripeptides By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP MF_00364
Pathway	Cell wall biogenesis; peptidoglycan recycling. HAMAP MF_00364
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan turnover Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Beta-hexosaminidase HAMAP MF_00364		PRO_0000234912
Sites
	Active site	248	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6D674-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: C0AA9F4511D32A45	FASTA	342	37,736
	10 20 30 40 50 60 MGPVMLDVAS YELDAEDREV LEHPLVGGVI LFTRNFHDVA QLRELVRQIR AASHERLVVS 70 80 90 100 110 120 VDQEGGRVQR FRDGFTRLPA AQAFAALNSV SEAQRLAEEG GWLMAAEMIA MDIDISFAPV 130 140 150 160 170 180 LDIGHQSAAI GERSFHEQPE MALAVAQSFI RGMHSAGMKV TGKHFPGHGA VSADSHKETP 190 200 210 220 230 240 RDPRPLAEIR AHDMLIFKAL IQSQQLDAIM PAHVIYTEAD PHPASGSSYW LKTVLREELG 250 260 270 280 290 300 FDGIIFSDDL SMEGAAVMGS YPERAQAALQ AGCDMILVCN HREGAISVLD NLSPVKAEQL 310 320 330 340 TRLYHQGSFS RRELLDSPRW KLANQALTSL SERWQAHKNG EK

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX950851 Genomic DNA. Translation: CAG74717.1.
RefSeq	YP_049911.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2884609.
GenomeReviews	Gene locus ECA1813 in contig BX950851_GR.
KEGG	eca:ECA1813.
NMPDR	fig|218491.3.peg.281.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6D674.
OMA	VERSIRM.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA1813-MON.
BRENDA	3.2.1.52. 281579.
Family and domain databases
HAMAP	MF_00364. [Tree]
InterPro	IPR019800. Glyco_hydro_3_AS. IPR001764. Glyco_hydro_3_N. [Graphical view]
Gene3D	G3DSA:3.20.20.300. Glyco_hydro_3_N. 1 hit.
Pfam	PF00933. Glyco_hydro_3. 1 hit. [Graphical view]
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGZ_ERWCT

Accession

Primary (citable) accession number: Q6D674

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	August 16, 2004
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)