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Q6D5Z2

- NAPA_PECAS

UniProt

Q6D5Z2 - NAPA_PECAS

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi46 – 461Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi49 – 491Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi53 – 531Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi81 – 811Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciPATR218491:GJNB-1935-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:ECA1896
    OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
    Taxonomic identifieri218491 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium
    ProteomesiUP000007966: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Tat-type signalUniRule annotationAdd
    BLAST
    Chaini32 – 828797Periplasmic nitrate reductasePRO_0000045984Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi218491.ECA1896.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6D5Z2.
    SMRiQ6D5Z2. Positions 37-827.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 95574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiHPKTRVA.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6D5Z2-1 [UniParc]FASTAAdd to Basket

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    MKLSRRHFMK ANAVAAAAAV AGITIPIAVR AATEQSDAIH WDKAPCRFCG    50
    VGCGVLVGTQ NGRIVASQGD PEAPVNRGLN CIKGYFLPKI MYGQDRLTQP 100
    LLRMRNGQFD KEGEFTPISW DKAFDIMAEK FKTALKEKGP NAIGMFGSGQ 150
    STIWEGYASA KLFKAGFRSN NIDPNARHCM ASAVVGFMRT FGMDEPMGCY 200
    DDIEQTDAFV LWGSNMAEMH PILWSRITDR RLSNSNVTVA VLSTYQHRSF 250
    ELADNGMVFT PQTDLAILNY IANYIIQNNA VNEAFFTRHV NLRRGVTDIG 300
    YGLRPTHPLE KAAKNPGSDA SEPMSFEEYK AFVADYTLEK TVAISGVPAD 350
    QLEALAKLYA DPKKKVISYW TMGFNQHTRG VWANNLVYNI HLLTGKISQP 400
    GCGPFSLTGQ PSACGTAREV GTFAHRLPAD MVVTNEKHRA IAEKLWQLPT 450
    GTIPEKIGLH AVAQDRALKD GTLNAYWVMC NNNMQAGPNI NQERMPGWRD 500
    PRNFIVVSDP YPTISALAAD LILPTAMWVE KEGAYGNAER RTQFWRQQVK 550
    APGESKSDLW QVVSFAKRFT VEDVWPEELL AQKPAYRGKT LYDVLFANDV 600
    TTRFPLSELA ENQLNDESRE FGFYLQKGLF EEYAAFGRGH GHDLAPFDAY 650
    HKVRGLRWPV VDGKETQWRY SEGHDPYVKA GEAYRFYGKP DGKAVIFALP 700
    YEPAAEAPDE EYDLWFSTGR VLEHWHTGSM TRRVPELHRA FPEAVLFIHP 750
    QDAKARDLRR GEKVRIISRR GEVISVVETR GRNKPPRGLV YMPFFDAAQM 800
    TNVLTLDATD PLSKETDFKK CAVKLAKV 828
    Length:828
    Mass (Da):92,780
    Last modified:August 16, 2004 - v1
    Checksum:i2F43B05328AD7A46
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX950851 Genomic DNA. Translation: CAG74799.1.
    RefSeqiWP_011093464.1. NC_004547.2.
    YP_049993.1. NC_004547.2.

    Genome annotation databases

    EnsemblBacteriaiCAG74799; CAG74799; ECA1896.
    GeneIDi2884039.
    KEGGieca:ECA1896.
    PATRICi20479076. VBIPecAtr54885_1926.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX950851 Genomic DNA. Translation: CAG74799.1 .
    RefSeqi WP_011093464.1. NC_004547.2.
    YP_049993.1. NC_004547.2.

    3D structure databases

    ProteinModelPortali Q6D5Z2.
    SMRi Q6D5Z2. Positions 37-827.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 218491.ECA1896.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAG74799 ; CAG74799 ; ECA1896 .
    GeneIDi 2884039.
    KEGGi eca:ECA1896.
    PATRICi 20479076. VBIPecAtr54885_1926.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi HPKTRVA.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci PATR218491:GJNB-1935-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SCRI 1043 / ATCC BAA-672.

    Entry informationi

    Entry nameiNAPA_PECAS
    AccessioniPrimary (citable) accession number: Q6D5Z2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 7, 2006
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3