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Q6D5Z2 (NAPA_PECAS) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:ECA1896
OrganismPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica) [Complete proteome] [HAMAP]
Taxonomic identifier218491 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium

Protein attributes

Sequence length828 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131Tat-type signal Potential
Chain32 – 828797Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000045984

Regions

Domain39 – 95574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding461Iron-sulfur (4Fe-4S) By similarity
Metal binding491Iron-sulfur (4Fe-4S) By similarity
Metal binding531Iron-sulfur (4Fe-4S) By similarity
Metal binding811Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6D5Z2 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 2F43B05328AD7A46

FASTA82892,780
        10         20         30         40         50         60 
MKLSRRHFMK ANAVAAAAAV AGITIPIAVR AATEQSDAIH WDKAPCRFCG VGCGVLVGTQ 

        70         80         90        100        110        120 
NGRIVASQGD PEAPVNRGLN CIKGYFLPKI MYGQDRLTQP LLRMRNGQFD KEGEFTPISW 

       130        140        150        160        170        180 
DKAFDIMAEK FKTALKEKGP NAIGMFGSGQ STIWEGYASA KLFKAGFRSN NIDPNARHCM 

       190        200        210        220        230        240 
ASAVVGFMRT FGMDEPMGCY DDIEQTDAFV LWGSNMAEMH PILWSRITDR RLSNSNVTVA 

       250        260        270        280        290        300 
VLSTYQHRSF ELADNGMVFT PQTDLAILNY IANYIIQNNA VNEAFFTRHV NLRRGVTDIG 

       310        320        330        340        350        360 
YGLRPTHPLE KAAKNPGSDA SEPMSFEEYK AFVADYTLEK TVAISGVPAD QLEALAKLYA 

       370        380        390        400        410        420 
DPKKKVISYW TMGFNQHTRG VWANNLVYNI HLLTGKISQP GCGPFSLTGQ PSACGTAREV 

       430        440        450        460        470        480 
GTFAHRLPAD MVVTNEKHRA IAEKLWQLPT GTIPEKIGLH AVAQDRALKD GTLNAYWVMC 

       490        500        510        520        530        540 
NNNMQAGPNI NQERMPGWRD PRNFIVVSDP YPTISALAAD LILPTAMWVE KEGAYGNAER 

       550        560        570        580        590        600 
RTQFWRQQVK APGESKSDLW QVVSFAKRFT VEDVWPEELL AQKPAYRGKT LYDVLFANDV 

       610        620        630        640        650        660 
TTRFPLSELA ENQLNDESRE FGFYLQKGLF EEYAAFGRGH GHDLAPFDAY HKVRGLRWPV 

       670        680        690        700        710        720 
VDGKETQWRY SEGHDPYVKA GEAYRFYGKP DGKAVIFALP YEPAAEAPDE EYDLWFSTGR 

       730        740        750        760        770        780 
VLEHWHTGSM TRRVPELHRA FPEAVLFIHP QDAKARDLRR GEKVRIISRR GEVISVVETR 

       790        800        810        820 
GRNKPPRGLV YMPFFDAAQM TNVLTLDATD PLSKETDFKK CAVKLAKV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX950851 Genomic DNA. Translation: CAG74799.1.
RefSeqYP_049993.1. NC_004547.2.

3D structure databases

ProteinModelPortalQ6D5Z2.
SMRQ6D5Z2. Positions 37-827.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING218491.ECA1896.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAG74799; CAG74799; ECA1896.
GeneID2884039.
KEGGeca:ECA1896.
PATRIC20479076. VBIPecAtr54885_1926.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMADAFGFYI.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycPATR218491:GJNB-1935-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_PECAS
AccessionPrimary (citable) accession number: Q6D5Z2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: August 16, 2004
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families