Q6D4X4 (ADD_ERWCT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine deaminase
EC=3.5.4.4

Alternative name(s):
Adenosine aminohydrolase

Gene names

Name:	add
Ordered Locus Names:	ECA2266

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Adenosine + H2O = inosine + NH3. HAMAP MF_00540
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00540
Sequence similarities	Belongs to the adenosine and AMP deaminases family. Adenosine deaminase subfamily.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	adenosine deaminase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Adenosine deaminase HAMAP MF_00540		PRO_1000017662
Sites
	Active site	200	1	Proton donor By similarity
	Metal binding	12	1	Zinc; catalytic By similarity
	Metal binding	14	1	Zinc; catalytic By similarity
	Metal binding	197	1	Zinc; catalytic By similarity
	Metal binding	278	1	Zinc; catalytic By similarity
	Binding site	14	1	Substrate By similarity
	Binding site	16	1	Substrate By similarity
	Binding site	170	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	279	1	Substrate By similarity
	Site	221	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6D4X4 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 4F7E104D63115D50	FASTA	337	37,126
	10 20 30 40 50 60 MIDLRLPLTD IHRHLDGNIR AQSILELGRQ YNIALPASDL DALRPHVQVT KNEPDLLSFL 70 80 90 100 110 120 QKLDWGVAVL GSLDACRRVA YENVEDAMNA GLDYAELRFS PYYMAMNHKL PIAGVVEAVI 130 140 150 160 170 180 DGITAGSRDF DTDIRLIGIM SRTFGTEACQ QELDALLSQR DRIVALDLAG DELGYPGAQF 190 200 210 220 230 240 TSHFQQARDA GWHITVHAGE AAGPESIWQA INHLGAERIG HGVTAIIDPR LMTHMAETGI 250 260 270 280 290 300 GIESCLTSNI QTSTVETLDK HPLIHFLRYG IPATINTDDP AVQGIEIRHE YEVAAPLAGL 310 320 330 TAVETRKAQE NGLNIAFISE QEKQQLREKV LRKRASA

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950851 Genomic DNA. Translation: CAG75169.1.
RefSeq	YP_050361.1. NC_004547.2.
3D structure databases
HSSP	HSSP built from PDB template 1A4M based on UniProtKB P03958.
ProteinModelPortal	Q6D4X4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2883901.
GenomeReviews	Gene locus ECA2266 in contig BX950851_GR.
KEGG	eca:ECA2266.
NMPDR	fig|218491.3.peg.1781.
PATRIC	20479820. VBIPecAtr54885_2296.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG630382.
OMA	PYYMAMN.
ProtClustDB	PRK09358.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA2266-MONOMER.
Family and domain databases
HAMAP	MF_00540. A_deaminase. [Tree]
InterPro	IPR001365. A/AMP_deaminase_dom. IPR006330. A_deaminase. [Graphical view]
KO	K01488.
PANTHER	PTHR11409:SF21. PTHR11409:SF21. 1 hit.
Pfam	PF00962. A_deaminase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01430. Aden_deam. 1 hit.
PROSITE	PS00485. A_DEAMINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADD_ERWCT

Accession

Primary (citable) accession number: Q6D4X4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 16, 2004
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program