Reviewed, UniProtKB/Swiss-Prot Q6D4N9 (DADA_ERWCT)

Names and origin

Protein names

Recommended name:
    D-amino acid dehydrogenase small subunit
    EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	ECA2351

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity.
Catalytic activity	A D-amino acid + H(2)O + acceptor = a 2-oxo acid + NH(3) + reduced acceptor.
Cofactor	FAD By similarity.
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1.
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the dadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	D-amino acid dehydrogenase small subunit		PRO_1000066095
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6D4N9-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 357FCFB7ABF7B949	FASTA	417	45,920
	10 20 30 40 50 60 MRVVILGSGV VGVSTAWYLA QAGHDVTVID RQPEPALETS AGNAGQISPG YAAPWAAPGI 70 80 90 100 110 120 PLKAIKWMFQ RHAPLAIRPD FTTAQLCWMW QMLLNCDARH YKTNKARMVR LAEYSRDCLQ 130 140 150 160 170 180 QLRRDTGIQY EGRQGGTLQL FRTEQQYDNA TRDIAVLEEA GVPYQLLARH ELASVEPALA 190 200 210 220 230 240 NIVGTLTGGL RLPHDETGDC QLFTRQLAAM AAEAGVTFKL GRNVRQLRVE GQSVTGVQCD 250 260 270 280 290 300 DEMIVADAYV MACGSYSTGL LRQWFDIPVY PLKGYSLTIP LADDASAPVS TVLDETYKVA 310 320 330 340 350 360 ITRFDNRIRV GGMAEVVGFN TDLNPKRRET LEMVVRDLYP HCGPIEQATF WTGLRPMTPD 370 380 390 400 410 GTPLVGRSPL KNLYLNTGHG TLGWTMACGS GKLLADILSD KSPEIEADDL SVSRYTR

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX950851 Genomic DNA. Translation: CAG75254.1.
RefSeq	YP_050446.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2882627.
GenomeReviews	Gene locus ECA2351 in contig BX950851_GR.
KEGG	eca:ECA2351.
NMPDR	fig|218491.3.peg.2006.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6D4N9.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA2351-MON.
Family and domain databases
HAMAP	MF_01202. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DADA_ERWCT

Accession

Primary (citable) accession number: Q6D4N9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 16, 2004
Last modified:	November 4, 2008
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)