Q6D4E2 (POTA_ERWCT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spermidine/putrescine import ATP-binding protein PotA
EC=3.6.3.31

Gene names

Name:	potA
Ordered Locus Names:	ECA2450

Organism

Erwinia carotovora subsp. atroseptica (strain SCRI 1043 / ATCC BAA-672) (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

218491 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium › Erwinia carotovora subsp. atroseptica

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system By similarity.
Catalytic activity	ATP + H2O + polyamine(Out) = ADP + phosphate + polyamine(In).
Subunit structure	The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD) Potential.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the ABC transporter superfamily. Spermidine/putrescine importer (TC 3.A.1.11.1) family. [View classification] Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	ATP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	ATP-binding cassette (ABC) transporter complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW putrescine-importing ATPase activity Inferred from electronic annotation. Source: InterPro spermidine-importing ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 383	383	Spermidine/putrescine import ATP-binding protein PotA		PRO_0000286215
Regions
	Domain	23 – 253	231	ABC transporter
	Nucleotide binding	55 – 62	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6D4E2 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: CD6815B7C96C071D	FASTA	383	43,512
	10 20 30 40 50 60 MICQIRKQSY TLMKENSLTV PVVELLNVHK GFDGKDIISH FNLTINNGEF LTILGPSGCG 70 80 90 100 110 120 KTTVLRLIAG LETVDSGNIM LEKQDITHQP AEQRHVNTVF QSYALFPHLS VFENVAFGLR 130 140 150 160 170 180 MQKTPAVEMT PRVIEALKMV QLDELAQRRP HQLSGGQQQR VAIARAVVNQ PKVLLLDESL 190 200 210 220 230 240 SALDYKLRKQ MQNELKALQR KLGITFIFVT HDQEEALTMS DRIVVMRDGR IEQDGTPREI 250 260 270 280 290 300 YEEPKNLFVA SFIGEINIFD AIVLDLLDEQ RVRALVEGHE CVITVSFLVN IGQHLNVLLR 310 320 330 340 350 360 PEDLRVEELG DGMQAEGMVG YVRERNYKGM TLESNIELEN GKMVMVSEFF NEDDPDFDHS 370 380 LDQKVDVTWV ESWEVVLHDE DVA

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950851 Genomic DNA. Translation: CAG75351.1.
RefSeq	YP_050543.1. NC_004547.2.
3D structure databases
HSSP	HSSP built from PDB template 2AWN based on UniProtKB P68187.
ProteinModelPortal	Q6D4E2.
ModBase	Search...
Protein-protein interaction databases
STRING	218491.ECA2450.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAG75351; CAG75351; ECA2450.
GeneID	2883184.
KEGG	eca:ECA2450.
PATRIC	20480192. VBIPecAtr54885_2481.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3842.
KO	K11072.
OMA	IGYIRER.
ProtClustDB	PRK09452.
Enzyme and pathway databases
BioCyc	PATR218491:GJNB-2492-MONOMER.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR017879. ABC_Sperm/Put_ATP-bd_PotA. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR008995. Mo/tungstate-bd_C_term_dom. IPR005893. Sp_pt_ABC_ATP-bd. IPR013611. Transp-assoc_OB_typ2. [Graphical view]
Pfam	PF00005. ABC_tran. 1 hit. PF08402. TOBE_2. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF50331. MOP_like. 1 hit.
TIGRFAMs	TIGR01187. potA. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51305. POTA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POTA_ERWCT

Accession

Primary (citable) accession number: Q6D4E2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program