Q6D4A0 (RUVC_ERWCT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Crossover junction endodeoxyribonuclease RuvC
EC=3.1.22.4

Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC

Gene names

Name:	ruvC
Ordered Locus Names:	ECA2493

Organism

Erwinia carotovora subsp. atroseptica (strain SCRI 1043 / ATCC BAA-672) (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

218491 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium › Erwinia carotovora subsp. atroseptica

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034
Catalytic activity	Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Sequence similarities	Belongs to the RuvC family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	crossover junction endodeoxyribonuclease activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 170	170	Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034		PRO_0000225144
Sites
	Metal binding	8	1	Magnesium By similarity
	Metal binding	67	1	Magnesium By similarity
	Metal binding	139	1	Magnesium By similarity
	Metal binding	142	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6D4A0 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 6427E633830F3251	FASTA	170	18,154
	10 20 30 40 50 60 MTIIVGIDPG SRVTGYGIIR QQGRHLTYLG SGCIRTVVDD MPTRLKLIYA GVSEIITQFQ 70 80 90 100 110 120 PDCMAIEQVF MAKNPDSALK LGQARGVAIV AGVNQDLPVF EYAARLVKKT VVGTGAADKK 130 140 150 160 170 QVQHMVRSLL KLSASPQADA ADALAIAITH CHFNQSLLRT AAVKVNPLAG

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950851 Genomic DNA. Translation: CAG75393.1.
RefSeq	YP_050585.1. NC_004547.2.
3D structure databases
ProteinModelPortal	Q6D4A0.
SMR	Q6D4A0. Positions 3-159.
ModBase	Search...
Protein-protein interaction databases
STRING	218491.ECA2493.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAG75393; CAG75393; ECA2493.
GeneID	2884439.
KEGG	eca:ECA2493.
PATRIC	20480277. VBIPecAtr54885_2523.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0817.
HOGENOM	HOG000012181.
KO	K01159.
OMA	YTALQMK.
ProtClustDB	PRK00039.
Enzyme and pathway databases
BioCyc	PATR218491:GJNB-2536-MONOMER.
Family and domain databases
HAMAP	MF_00034. RuvC.
InterPro	IPR012337. RNaseH-like_dom. IPR020563. X-over_junc_endoDNase_Mg_BS. IPR002176. X-over_junc_endoDNase_RuvC. [Graphical view]
Pfam	PF02075. RuvC. 1 hit. [Graphical view]
PRINTS	PR00696. RSOLVASERUVC.
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
TIGRFAMs	TIGR00228. ruvC. 1 hit.
PROSITE	PS01321. RUVC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RUVC_ERWCT

Accession

Primary (citable) accession number: Q6D4A0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program