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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81MagnesiumUniRule annotation
Metal bindingi67 – 671MagnesiumUniRule annotation
Metal bindingi139 – 1391MagnesiumUniRule annotation
Metal bindingi142 – 1421MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciPATR218491:GJNB-2536-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:ECA2493
OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Taxonomic identifieri218491 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium
ProteomesiUP000007966 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 170170Crossover junction endodeoxyribonuclease RuvCPRO_0000225144Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi218491.ECA2493.

Structurei

3D structure databases

ProteinModelPortaliQ6D4A0.
SMRiQ6D4A0. Positions 3-159.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
KOiK01159.
OMAiYTALQMK.
OrthoDBiEOG6RG044.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6D4A0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIIVGIDPG SRVTGYGIIR QQGRHLTYLG SGCIRTVVDD MPTRLKLIYA
60 70 80 90 100
GVSEIITQFQ PDCMAIEQVF MAKNPDSALK LGQARGVAIV AGVNQDLPVF
110 120 130 140 150
EYAARLVKKT VVGTGAADKK QVQHMVRSLL KLSASPQADA ADALAIAITH
160 170
CHFNQSLLRT AAVKVNPLAG
Length:170
Mass (Da):18,154
Last modified:August 16, 2004 - v1
Checksum:i6427E633830F3251
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950851 Genomic DNA. Translation: CAG75393.1.
RefSeqiWP_011094040.1. NC_004547.2.
YP_050585.1. NC_004547.2.

Genome annotation databases

EnsemblBacteriaiCAG75393; CAG75393; ECA2493.
GeneIDi2884439.
KEGGieca:ECA2493.
PATRICi20480277. VBIPecAtr54885_2523.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950851 Genomic DNA. Translation: CAG75393.1.
RefSeqiWP_011094040.1. NC_004547.2.
YP_050585.1. NC_004547.2.

3D structure databases

ProteinModelPortaliQ6D4A0.
SMRiQ6D4A0. Positions 3-159.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi218491.ECA2493.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG75393; CAG75393; ECA2493.
GeneIDi2884439.
KEGGieca:ECA2493.
PATRICi20480277. VBIPecAtr54885_2523.

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
KOiK01159.
OMAiYTALQMK.
OrthoDBiEOG6RG044.

Enzyme and pathway databases

BioCyciPATR218491:GJNB-2536-MONOMER.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SCRI 1043 / ATCC BAA-672.

Entry informationi

Entry nameiRUVC_PECAS
AccessioniPrimary (citable) accession number: Q6D4A0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: August 16, 2004
Last modified: April 29, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.