Reviewed, UniProtKB/Swiss-Prot Q6D453 (SYN_ERWCT)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	ECA2541

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_0000176410

Sequences

	Sequence		Length	Mass (Da)
	Q6D453-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: C25ECD6C6EB22650	FASTA	466	52,317
	10 20 30 40 50 60 MSVVPVVDVL QGRVAVDSEV TVRGWVRTRR DSKAGISFIA VYDGSCFNPL QAVVNNNLSN 70 80 90 100 110 120 YQDDVLRLTT GCSVEITGNV VASPGEGQSF ELQATNVNVV GWVDDPDTYP MAAKRHSIEY 130 140 150 160 170 180 LREVAHLRPR TNLIGAVARV RHTLAQAIHR FFHENGYFWV STPLITASDT EGAGEMFRVS 190 200 210 220 230 240 TLDMENLPRT EQGKVDFSKD FFGKEAFLTV SGQLNGETYA CALSNVYTFG PTFRAENSNT 250 260 270 280 290 300 ARHLAEFWMI EPEVAFASLD DVAALAENLL KYVFQAVLNE RADDMAFFAE RVDKEAVTRL 310 320 330 340 350 360 EKFVSSDFAQ VDYTDAVEIL LNCGQQFENP VYWGVDLSSE HERYLAEQHF KAPVVVKNYP 370 380 390 400 410 420 KDIKAFYMRM NDDGKTVAAM DVLAPGIGEI IGGSQREERL AALDSRLEEM GLNKEDYWWY 430 440 450 460 RDLRRYGTIP HSGFGLGFER LIAYVTGVQN VRDVIPFPRT PRNASF

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950851 Genomic DNA. Translation: CAG75440.1.
RefSeq	YP_050632.1.
3D structure databases
SMR	Q6D453. Positions 16-464.
ModBase	Search...
Genome annotation databases
GeneID	2885587.
GenomeReviews	Gene locus ECA2541 in contig BX950851_GR.
KEGG	eca:ECA2541.
NMPDR	fig|218491.3.peg.1527.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG745843.
OMA	LQKKRHS.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA2541-MONOMER.
BRENDA	6.1.1.22. 281579.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_ERWCT

Accession

Primary (citable) accession number: Q6D453

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	August 16, 2004
Last modified:	February 9, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)