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Protein

Tetraacyldisaccharide 4'-kinase

Gene

lpxK

Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).UniRule annotation

Catalytic activityi

ATP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-4-O-phospho-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate).UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase (lpxD)
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi55 – 62ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPATR218491:G1G3T-2607-MONOMER
UniPathwayiUPA00359; UER00482

Names & Taxonomyi

Protein namesi
Recommended name:
Tetraacyldisaccharide 4'-kinaseUniRule annotation (EC:2.7.1.130UniRule annotation)
Alternative name(s):
Lipid A 4'-kinaseUniRule annotation
Gene namesi
Name:lpxKUniRule annotation
Ordered Locus Names:ECA2556
OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Taxonomic identifieri218491 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaePectobacterium
Proteomesi
  • UP000007966 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002299551 – 333Tetraacyldisaccharide 4'-kinaseAdd BLAST333

Proteomic databases

PRIDEiQ6D438

Interactioni

Protein-protein interaction databases

STRINGi218491.ECA2556

Structurei

3D structure databases

SMRiQ6D438
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxK family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CHD Bacteria
COG1663 LUCA
HOGENOMiHOG000004953
KOiK00912
OMAiMDDGFQN
OrthoDBiPOG091H000L

Family and domain databases

HAMAPiMF_00409 LpxK, 1 hit
InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR003758 Tetraacyldisaccharide_4-kinase
PANTHERiPTHR42724 PTHR42724, 1 hit
PfamiView protein in Pfam
PF02606 LpxK, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00682 lpxK, 1 hit

Sequencei

Sequence statusi: Complete.

Q6D438-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIERIWSGQS RLYWLLLPLS WLYGLITFLI RQSYRLGWRK SWRSPVPIVV
60 70 80 90 100
VGNLTAGGNG KTPVVIWLVE QLQRRGYRVG VVSRGYGGKA ERYPLLLNES
110 120 130 140 150
VTTVQAGDEP VLIFQRTGAP VAVAPRRAEA VSALLARHTL DVVITDDGLQ
160 170 180 190 200
HYALARDIEL VVIDGMRRFG NGWWLPAGPM RERESRLASV DAVVVNGGVP
210 220 230 240 250
QTNEIGMTLT AGMAVNLLSG ESRSLSQLYD VVAMAGIGHP PRFFATLRDA
260 270 280 290 300
GVSIAREVAF ADHQSYQPEQ LALLTQDTMQ PLLMTEKDAV KCKAFAQDNW
310 320 330
WYLPVDAVLA EPQGTQLLDK LEDMLNRNVG SRT
Length:333
Mass (Da):36,921
Last modified:August 16, 2004 - v1
Checksum:i782FB9745339BE1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950851 Genomic DNA Translation: CAG75455.1
RefSeqiWP_011094101.1, NC_004547.2

Genome annotation databases

EnsemblBacteriaiCAG75455; CAG75455; ECA2556
KEGGieca:ECA2556
PATRICifig|218491.5.peg.2591

Similar proteinsi

Entry informationi

Entry nameiLPXK_PECAS
AccessioniPrimary (citable) accession number: Q6D438
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: August 16, 2004
Last modified: May 23, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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