Reviewed, UniProtKB/Swiss-Prot Q6D3B3 (MAO1_ERWCT)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	ECA2831

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	NAD-dependent malic enzyme HAMAP MF_01619		PRO_0000160218
Sites
	Active site	104	1	Proton donor By similarity
	Active site	175	1	Proton acceptor By similarity
	Metal binding	246	1	Divalent metal cation By similarity
	Metal binding	247	1	Divalent metal cation By similarity
	Metal binding	270	1	Divalent metal cation By similarity
	Binding site	157	1	NAD By similarity
	Binding site	270	1	NAD By similarity
	Binding site	418	1	NAD By similarity
	Site	270	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6D3B3-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 697B4BAC259D4AD2	FASTA	565	62,720
	10 20 30 40 50 60 MELEYESKRP LYIPYAGPIL LEFPLLNKGS AFTEEERADF NLAGLLPEAV ETIEEQAERA 70 80 90 100 110 120 WRQYQEFKHD IEKHVYLRNI QDTNETLFYR LLDGHLNEMM PIIYTPTVGE ACEHFSDIYR 130 140 150 160 170 180 RARGLFISYP NRANIDDMLQ NATKQNVKVI VVTDGERILG LGDQGIGGMG IPIGKLALYT 190 200 210 220 230 240 ACGGISPAYT LPVVLDVGTN NPQRLNDPLY MGWRHPRITD DEYYAFVDEF IQAVKRRWPN 250 260 270 280 290 300 VLLQFEDFAQ KNATPLLNRY RDEICSFNDD IQGTAAVAIG SLIAASRAAG TQLRDQTVAF 310 320 330 340 350 360 LGAGSAGCGI AEQIIAQMKS EGLSEEEARA RVFMVDRFGL LTDKLPNLLD FQSKLVQKSE 370 380 390 400 410 420 LLADWDCNSD AISLLEVVRN AKPTIMIGVS GQPGLFTEEI IREMYKHCAR PIVMPLSNPT 430 440 450 460 470 480 SRVEARPEDI IRWTEGSALV ATGSPFSPVH YQDKVFPIAQ CNNSYIFPGI GLGVLASGAN 490 500 510 520 530 540 RITDGMLMAA SRALADCSPL ANNGEGALLP DLSDIQQVSK RIALDVGKAA QLQGVAVVTS 550 560 ADALQKAIDH NFWQPQYRSY KRTSF

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX950851 Genomic DNA. Translation: CAG75731.1.
RefSeq	YP_050922.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2884400.
GenomeReviews	Gene locus ECA2831 in contig BX950851_GR.
KEGG	eca:ECA2831.
NMPDR	fig|218491.3.peg.898.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6D3B3.
OMA	Q6D3B3. IQDNNET.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA2831-MON.
BRENDA	1.1.1.38. 281579.
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_ERWCT

Accession

Primary (citable) accession number: Q6D3B3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	August 16, 2004
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)