Reviewed, UniProtKB/Swiss-Prot Q6D2L7 (FADJ_ERWCT)

Names and origin

Protein names

Recommended name:
    Fatty acid oxidation complex subunit alpha
Including the following 2 domains:
    1- Recommended name:
            Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase
              EC=4.2.1.17
              EC=5.1.2.3
    2- Recommended name:
            3-hydroxyacyl-CoA dehydrogenase
              EC=1.1.1.35

Gene names

Name:	fadJ
Ordered Locus Names:	ECA3078

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	731 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities By similarity.
Catalytic activity	(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O. HAMAP MF_01617 (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH. HAMAP MF_01617 (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA. HAMAP MF_01617
Pathway	Lipid metabolism; fatty acid beta-oxidation. HAMAP MF_01617
Subunit structure	Heterotetramer of two alpha chains (fadJ) and two beta chains (fadI) By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid degradation Lipid metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Isomerase Lyase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid beta-oxidation Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	fatty acid beta-oxidation multienzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	3-hydroxyacyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: HAMAP 3-hydroxybutyryl-CoA epimerase activity Inferred from electronic annotation. Source: HAMAP NAD or NADH binding Inferred from electronic annotation. Source: InterPro enoyl-CoA hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 731	731	Fatty acid oxidation complex subunit alpha HAMAP MF_01617		PRO_0000109297
Regions
	Region	15 – 204	190	Enoyl-CoA hydratase By similarity
	Region	320 – 729	410	3-hydroxyacyl-CoA dehydrogenase By similarity
Sites
	Site	132	1	Important for catalytic activity By similarity
	Site	154	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6D2L7-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: DAAB4DA0B42ECCB3	FASTA	731	80,047
	10 20 30 40 50 60 MNDQQPFSVT ASPTTEKTSA FSLTIRPDNI GVISIDVPGE RVNTLKSEFA EQILSVFELA 70 80 90 100 110 120 RQHATLRGLI FISAKPDSFI AGADITMLNK CSSAEQAENL AKQGQETFDQ IAALPFPVVA 130 140 150 160 170 180 AIHGACLGGG LELALACDYR VCSLDEKTVL GLPEVQLGLL PGSGGTQRLP RLIGLDSALD 190 200 210 220 230 240 LILTGRHLRA GQALRQGLVD EAVPHDILLD TAVEILKKGK RKAVPLGWRS RLLNSPGIRH 250 260 270 280 290 300 LLFKMVKRKT RAKTHGNYPA TEKIIQVVRR GIEKGREEGY RHEARAFGKL VMTPESAALR 310 320 330 340 350 360 HLFFATNALK KTSGAASEAK PIHRVGILGG GLMGGGIASV TATRGQLPVR IKDINEQGIN 370 380 390 400 410 420 HALKYNWQLL TKRVQSKRMK PTERQRLMTL ISGSTDYRGF EHADIVIEAV FEDLALKRQM 430 440 450 460 470 480 ITEIEDHAAP HTIFASNTSS LPIHQIAEGA RRPQLVVGLH YFSPVDKMPL VEVIPHAHTS 490 500 510 520 530 540 AETVATTVAL ARKQGKTAIV VGDSAGFYVN RILAPYINEA AYCLLEGEPI ESIDYALVRF 550 560 570 580 590 600 GFPVGPLALL DEVGIDVATK IVPVLSEELG DRFTSPPAFD AILKDGRKGR KNGKGFYRYN 610 620 630 640 650 660 KTRRFWHSGK EVDSSVYPLL DVTPKAHIDP ALISQRAVMM MLNEAARCLD EGVIQCARDG 670 680 690 700 710 720 DIGAVFGIGF PPFLGGPFHY MDRLGMETVV KTLLVLQQQY GDRFAPCERL LTMREGQRTF 730 YPPADKDNSI S

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX950851 Genomic DNA. Translation: CAG75977.1.
RefSeq	YP_051168.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2883878.
GenomeReviews	Gene locus ECA3078 in contig BX950851_GR.
KEGG	eca:ECA3078.
NMPDR	fig|218491.3.peg.1434.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6D2L7.
OMA	GDRFTPC.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA3078-MON.
BRENDA	1.1.1.35. 281579. 4.2.1.17. 281579. 5.1.2.3. 281579.
Family and domain databases
HAMAP	MF_01617. [Tree]
InterPro	IPR006180. 3-OHacyl-CoA_DH_CS. IPR006176. 3-OHacyl-CoA_DH_NAD-bd. IPR006108. 3HC_DH_C. IPR001753. Crotonase_core. IPR013328. DH_multihelical. IPR018376. Enoyl-CoA_hyd/isom_CS. IPR012802. FadJ. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 2 hits.
Pfam	PF00725. 3HCDH. 2 hits. PF02737. 3HCDH_N. 1 hit. PF00378. ECH. 1 hit. [Graphical view]
TIGRFAMs	TIGR02440. FadJ. 1 hit.
PROSITE	PS00067. 3HCDH. 1 hit. PS00166. ENOYL_COA_HYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FADJ_ERWCT

Accession

Primary (citable) accession number: Q6D2L7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	August 16, 2004
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)