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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.

Cofactori

Mg2+

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei52Substrate; via amide nitrogen1
Binding sitei127ATP1
Binding sitei173ATP1
Binding sitei200Substrate; via amide nitrogen1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinase (EC:2.7.1.50)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name:
TH kinase
Short name:
Thz kinase
Gene namesi
Name:thiM
Ordered Locus Names:ECA3197
OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Taxonomic identifieri218491 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaePectobacterium
Proteomesi
  • UP000007966 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003365551 – 264Hydroxyethylthiazole kinaseAdd BLAST264

Interactioni

Protein-protein interaction databases

STRINGi218491.ECA3197.

Structurei

3D structure databases

ProteinModelPortaliQ6D299.
SMRiQ6D299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.

Phylogenomic databases

eggNOGiENOG4105C4U. Bacteria.
COG2145. LUCA.
HOGENOMiHOG000114352.
KOiK00878.
OMAiKRPLVHN.
OrthoDBiPOG091H004S.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
InterProiView protein in InterPro
IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
PANTHERiPTHR44423. PTHR44423. 1 hit.
PfamiView protein in Pfam
PF02110. HK. 1 hit.
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6D299-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTQPADFSA AQAATSLTQF RSAAPLVHCL TNDVVQSFTA NVLLALNASP
60 70 80 90 100
AMVVDPEEAA QFSAVADALL INVGTLERTR AEAMRAAVNS AHQAGTPWVL
110 120 130 140 150
DPVAVGGLTF RTEFCRELLT WKPAAIRGNA SEIMALAGLA AQGRGVDSAD
160 170 180 190 200
DSLAALPAAR ELARQVGTIV VVTGAVDYVT DGERDIAVTG GDSMMTRVVG
210 220 230 240 250
TGCALSAVVA GFCSLEGDRL SHVTAACYVM ALAGQQATSV SQGTGSFIPH
260
FLDRLYTLRA EDLA
Length:264
Mass (Da):27,341
Last modified:August 16, 2004 - v1
Checksum:i19496665FE340316
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950851 Genomic DNA. Translation: CAG76095.1.
RefSeqiWP_011094718.1. NC_004547.2.

Genome annotation databases

EnsemblBacteriaiCAG76095; CAG76095; ECA3197.
KEGGieca:ECA3197.
PATRICifig|218491.5.peg.3239.

Similar proteinsi

Entry informationi

Entry nameiTHIM_PECAS
AccessioniPrimary (citable) accession number: Q6D299
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 16, 2004
Last modified: November 22, 2017
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families