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Q6D219

- RNC_PECAS

UniProt

Q6D219 - RNC_PECAS

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Protein
Ribonuclease 3
Gene
rnc, ECA3278
Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism By similarity.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+ By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi41 – 411Magnesium By similarity
Active sitei45 – 451 Reviewed prediction
Metal bindingi114 – 1141Magnesium By similarity
Active sitei117 – 1171 By similarity
Metal bindingi117 – 1171Magnesium By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. rRNA binding Source: UniProtKB-KW
  3. ribonuclease III activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. mRNA processing Source: UniProtKB-HAMAP
  2. rRNA catabolic process Source: InterPro
  3. rRNA processing Source: UniProtKB-HAMAP
  4. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing, rRNA processing, tRNA processing

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciPATR218491:GJNB-3328-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3 (EC:3.1.26.3)
Alternative name(s):
Ribonuclease III
Short name:
RNase III
Gene namesi
Name:rnc
Ordered Locus Names:ECA3278
OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Taxonomic identifieri218491 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium
ProteomesiUP000007966: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 226226Ribonuclease 3UniRule annotation
PRO_0000228531Add
BLAST

Proteomic databases

PRIDEiQ6D219.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi218491.ECA3278.

Structurei

3D structure databases

ProteinModelPortaliQ6D219.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 128123RNase III
Add
BLAST
Domaini155 – 22571DRBM
Add
BLAST

Sequence similaritiesi

Contains 1 RNase III domain.

Phylogenomic databases

eggNOGiCOG0571.
HOGENOMiHOG000246809.
KOiK03685.
OMAiAQKDPKT.
OrthoDBiEOG6T1WVS.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6D219-1 [UniParc]FASTAAdd to Basket

« Hide

MNPILINRLQ RKLGYTFQQY ELLLQALTHR SASSKHNERL EFLGDSILSF    50
VIANALYDRF PKVDEGDMSR MRATLVRGNT LAEIAREFEL GECLRLGPGE 100
LKSGGFRRES ILADTVEALI GGIFLDSDIL NIERLILNWY QTRLDEISPG 150
DKQKDPKTRL QEFLQGRHLP LPTYLVVQVR GEAHDQEFTI HCQVSGFSES 200
VIGTGSSRRK AEQAAAEQAL KKLELE 226
Length:226
Mass (Da):25,599
Last modified:August 16, 2004 - v1
Checksum:i4EC6F572717A7949
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX950851 Genomic DNA. Translation: CAG76176.1.
RefSeqiWP_011094796.1. NC_004547.2.
YP_051367.1. NC_004547.2.

Genome annotation databases

EnsemblBacteriaiCAG76176; CAG76176; ECA3278.
GeneIDi2885047.
KEGGieca:ECA3278.
PATRICi20481897. VBIPecAtr54885_3324.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX950851 Genomic DNA. Translation: CAG76176.1 .
RefSeqi WP_011094796.1. NC_004547.2.
YP_051367.1. NC_004547.2.

3D structure databases

ProteinModelPortali Q6D219.
ModBasei Search...

Protein-protein interaction databases

STRINGi 218491.ECA3278.

Proteomic databases

PRIDEi Q6D219.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAG76176 ; CAG76176 ; ECA3278 .
GeneIDi 2885047.
KEGGi eca:ECA3278.
PATRICi 20481897. VBIPecAtr54885_3324.

Phylogenomic databases

eggNOGi COG0571.
HOGENOMi HOG000246809.
KOi K03685.
OMAi AQKDPKT.
OrthoDBi EOG6T1WVS.

Enzyme and pathway databases

BioCyci PATR218491:GJNB-3328-MONOMER.

Family and domain databases

Gene3Di 1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPi MF_00104. RNase_III.
InterProi IPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view ]
PANTHERi PTHR11207. PTHR11207. 1 hit.
Pfami PF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view ]
SMARTi SM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view ]
SUPFAMi SSF69065. SSF69065. 1 hit.
TIGRFAMsi TIGR02191. RNaseIII. 1 hit.
PROSITEi PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SCRI 1043 / ATCC BAA-672.

Entry informationi

Entry nameiRNC_PECAS
AccessioniPrimary (citable) accession number: Q6D219
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: August 16, 2004
Last modified: September 3, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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