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Q6D1A4

- CYSG1_PECAS

UniProt

Q6D1A4 - CYSG1_PECAS

Protein

Siroheme synthase 1

Gene

cysG1

Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotation
    S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotation
    Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotation
    Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei231 – 2311S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Active sitei254 – 2541Proton acceptorUniRule annotation
    Active sitei276 – 2761Proton donorUniRule annotation
    Binding sitei312 – 3121S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei389 – 3891S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
    Binding sitei418 – 4181S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi22 – 232NADUniRule annotation
    Nucleotide bindingi43 – 442NADUniRule annotation

    GO - Molecular functioni

    1. NAD binding Source: InterPro
    2. precorrin-2 dehydrogenase activity Source: UniProtKB-HAMAP
    3. sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC
    4. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cobalamin biosynthetic process Source: UniProtKB-HAMAP
    2. siroheme biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase, Methyltransferase, Oxidoreductase, Transferase

    Keywords - Biological processi

    Cobalamin biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    NAD, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciPATR218491:GJNB-3618-MONOMER.
    UniPathwayiUPA00148; UER00211.
    UPA00148; UER00222.
    UPA00262; UER00211.
    UPA00262; UER00222.
    UPA00262; UER00376.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Siroheme synthase 1UniRule annotation
    Including the following 3 domains:
    Uroporphyrinogen-III C-methyltransferase 1UniRule annotation (EC:2.1.1.107UniRule annotation)
    Short name:
    Urogen III methylase 1UniRule annotation
    Alternative name(s):
    SUMT 1UniRule annotation
    Uroporphyrinogen III methylase 1UniRule annotation
    Short name:
    UROM 1UniRule annotation
    Precorrin-2 dehydrogenase 1UniRule annotation (EC:1.3.1.76UniRule annotation)
    Sirohydrochlorin ferrochelatase 1UniRule annotation (EC:4.99.1.4UniRule annotation)
    Gene namesi
    Name:cysG1UniRule annotation
    Ordered Locus Names:ECA3544
    OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
    Taxonomic identifieri218491 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium
    ProteomesiUP000007966: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 480480Siroheme synthase 1PRO_0000330505Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei128 – 1281PhosphoserineUniRule annotation

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi218491.ECA3544.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6D1A4.
    SMRiQ6D1A4. Positions 1-463.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 203203precorrin-2 dehydrogenase / sirohydrochlorin ferrochelataseUniRule annotationAdd
    BLAST
    Regioni222 – 480259Uroporphyrinogen-III C-methyltransferaseUniRule annotationAdd
    BLAST
    Regioni307 – 3093S-adenosyl-L-methionine bindingUniRule annotation
    Regioni337 – 3382S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.UniRule annotation
    In the C-terminal section; belongs to the precorrin methyltransferase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0007.
    HOGENOMiHOG000290518.
    KOiK02302.
    OMAiLHQQLAW.
    OrthoDBiEOG6DRPFR.

    Family and domain databases

    Gene3Di1.10.8.210. 1 hit.
    3.30.950.10. 1 hit.
    3.40.1010.10. 1 hit.
    3.40.50.720. 1 hit.
    HAMAPiMF_01646. Siroheme_synth.
    InterProiIPR000878. 4pyrrol_Mease.
    IPR014777. 4pyrrole_Mease_sub1.
    IPR014776. 4pyrrole_Mease_sub2.
    IPR006366. CobA/CysG_C.
    IPR016040. NAD(P)-bd_dom.
    IPR012409. Sirohaem_synth.
    IPR019478. Sirohaem_synthase_dimer_dom.
    IPR006367. Sirohaem_synthase_N.
    IPR003043. Uropor_MeTrfase_CS.
    [Graphical view]
    PfamiPF10414. CysG_dimeriser. 1 hit.
    PF13241. NAD_binding_7. 1 hit.
    PF00590. TP_methylase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
    SUPFAMiSSF53790. SSF53790. 1 hit.
    TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
    TIGR01470. cysG_Nterm. 1 hit.
    PROSITEiPS00839. SUMT_1. 1 hit.
    PS00840. SUMT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6D1A4-1 [UniParc]FASTAAdd to Basket

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    MNYLPIFADL RQRPVLVVGG GEVATRKIDL LQRAGAEVKI VALALAEPLA    50
    AQHQAGQVEW LAQSFTPELL SGVFLVIAAT DDAELNAAVF EAANQRHLLV 100
    NVVDDQPKCT FIFPSIVDRS PLVVAISSGG QAPVLARLLR EKLESLLPAS 150
    LGTMAEIAGS WRDRIKTRLH SMPARRRFWE RLFVGRFASL VSAGQLEQAE 200
    GELQQQLVNQ QDEQQLPAAA RGEVALVGAG PGDAGLLTLR GLQVMQQADV 250
    VLYDHLVSAD VLDLVRRDAE RICVGKRAST HSLPQGEINQ LLVKLAQEGK 300
    RVVRLKGGDP FIFGRGGEEL QAVAQAGITF QVVPGVTAAA GVTAYAGIPL 350
    THRDHAQSVI FITGHCRPDG DSLDWSTLAR GRQTLAIYMG TMKAAEISQQ 400
    LIAHGRSAQT PVAVISRGTR HDQQVQIGTL QELEHLARQA PTPALLVIGE 450
    VVDLHHQIAW FGQTTPTVPQ DSRPAVVNLA 480
    Length:480
    Mass (Da):51,737
    Last modified:August 16, 2004 - v1
    Checksum:i34E423B4160BB316
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX950851 Genomic DNA. Translation: CAG76442.1.
    RefSeqiYP_051632.1. NC_004547.2.

    Genome annotation databases

    EnsemblBacteriaiCAG76442; CAG76442; ECA3544.
    GeneIDi2882397.
    KEGGieca:ECA3544.
    PATRICi20482475. VBIPecAtr54885_3591.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX950851 Genomic DNA. Translation: CAG76442.1 .
    RefSeqi YP_051632.1. NC_004547.2.

    3D structure databases

    ProteinModelPortali Q6D1A4.
    SMRi Q6D1A4. Positions 1-463.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 218491.ECA3544.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAG76442 ; CAG76442 ; ECA3544 .
    GeneIDi 2882397.
    KEGGi eca:ECA3544.
    PATRICi 20482475. VBIPecAtr54885_3591.

    Phylogenomic databases

    eggNOGi COG0007.
    HOGENOMi HOG000290518.
    KOi K02302.
    OMAi LHQQLAW.
    OrthoDBi EOG6DRPFR.

    Enzyme and pathway databases

    UniPathwayi UPA00148 ; UER00211 .
    UPA00148 ; UER00222 .
    UPA00262 ; UER00211 .
    UPA00262 ; UER00222 .
    UPA00262 ; UER00376 .
    BioCyci PATR218491:GJNB-3618-MONOMER.

    Family and domain databases

    Gene3Di 1.10.8.210. 1 hit.
    3.30.950.10. 1 hit.
    3.40.1010.10. 1 hit.
    3.40.50.720. 1 hit.
    HAMAPi MF_01646. Siroheme_synth.
    InterProi IPR000878. 4pyrrol_Mease.
    IPR014777. 4pyrrole_Mease_sub1.
    IPR014776. 4pyrrole_Mease_sub2.
    IPR006366. CobA/CysG_C.
    IPR016040. NAD(P)-bd_dom.
    IPR012409. Sirohaem_synth.
    IPR019478. Sirohaem_synthase_dimer_dom.
    IPR006367. Sirohaem_synthase_N.
    IPR003043. Uropor_MeTrfase_CS.
    [Graphical view ]
    Pfami PF10414. CysG_dimeriser. 1 hit.
    PF13241. NAD_binding_7. 1 hit.
    PF00590. TP_methylase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036426. Sirohaem_synth. 1 hit.
    SUPFAMi SSF53790. SSF53790. 1 hit.
    TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
    TIGR01470. cysG_Nterm. 1 hit.
    PROSITEi PS00839. SUMT_1. 1 hit.
    PS00840. SUMT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SCRI 1043 / ATCC BAA-672.

    Entry informationi

    Entry nameiCYSG1_PECAS
    AccessioniPrimary (citable) accession number: Q6D1A4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3