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Q6D1A4

- CYSG1_PECAS

UniProt

Q6D1A4 - CYSG1_PECAS

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Protein

Siroheme synthase 1

Gene

cysG1

Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotation
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei231 – 2311S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei254 – 2541Proton acceptorUniRule annotation
Active sitei276 – 2761Proton donorUniRule annotation
Binding sitei312 – 3121S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei389 – 3891S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei418 – 4181S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 232NADUniRule annotation
Nucleotide bindingi43 – 442NADUniRule annotation

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. precorrin-2 dehydrogenase activity Source: UniProtKB-HAMAP
  3. sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC
  4. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-HAMAP
  2. siroheme biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NAD, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPATR218491:GJNB-3618-MONOMER.
UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthase 1UniRule annotation
Including the following 3 domains:
Uroporphyrinogen-III C-methyltransferase 1UniRule annotation (EC:2.1.1.107UniRule annotation)
Short name:
Urogen III methylase 1UniRule annotation
Alternative name(s):
SUMT 1UniRule annotation
Uroporphyrinogen III methylase 1UniRule annotation
Short name:
UROM 1UniRule annotation
Precorrin-2 dehydrogenase 1UniRule annotation (EC:1.3.1.76UniRule annotation)
Sirohydrochlorin ferrochelatase 1UniRule annotation (EC:4.99.1.4UniRule annotation)
Gene namesi
Name:cysG1UniRule annotation
Ordered Locus Names:ECA3544
OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Taxonomic identifieri218491 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium
ProteomesiUP000007966: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 480480Siroheme synthase 1PRO_0000330505Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281PhosphoserineUniRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi218491.ECA3544.

Structurei

3D structure databases

ProteinModelPortaliQ6D1A4.
SMRiQ6D1A4. Positions 1-463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 203203precorrin-2 dehydrogenase / sirohydrochlorin ferrochelataseUniRule annotationAdd
BLAST
Regioni222 – 480259Uroporphyrinogen-III C-methyltransferaseUniRule annotationAdd
BLAST
Regioni307 – 3093S-adenosyl-L-methionine bindingUniRule annotation
Regioni337 – 3382S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.UniRule annotation
In the C-terminal section; belongs to the precorrin methyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000290518.
KOiK02302.
OMAiLHQQLAW.
OrthoDBiEOG6DRPFR.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6D1A4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNYLPIFADL RQRPVLVVGG GEVATRKIDL LQRAGAEVKI VALALAEPLA
60 70 80 90 100
AQHQAGQVEW LAQSFTPELL SGVFLVIAAT DDAELNAAVF EAANQRHLLV
110 120 130 140 150
NVVDDQPKCT FIFPSIVDRS PLVVAISSGG QAPVLARLLR EKLESLLPAS
160 170 180 190 200
LGTMAEIAGS WRDRIKTRLH SMPARRRFWE RLFVGRFASL VSAGQLEQAE
210 220 230 240 250
GELQQQLVNQ QDEQQLPAAA RGEVALVGAG PGDAGLLTLR GLQVMQQADV
260 270 280 290 300
VLYDHLVSAD VLDLVRRDAE RICVGKRAST HSLPQGEINQ LLVKLAQEGK
310 320 330 340 350
RVVRLKGGDP FIFGRGGEEL QAVAQAGITF QVVPGVTAAA GVTAYAGIPL
360 370 380 390 400
THRDHAQSVI FITGHCRPDG DSLDWSTLAR GRQTLAIYMG TMKAAEISQQ
410 420 430 440 450
LIAHGRSAQT PVAVISRGTR HDQQVQIGTL QELEHLARQA PTPALLVIGE
460 470 480
VVDLHHQIAW FGQTTPTVPQ DSRPAVVNLA
Length:480
Mass (Da):51,737
Last modified:August 16, 2004 - v1
Checksum:i34E423B4160BB316
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX950851 Genomic DNA. Translation: CAG76442.1.
RefSeqiWP_011095047.1. NC_004547.2.
YP_051632.1. NC_004547.2.

Genome annotation databases

EnsemblBacteriaiCAG76442; CAG76442; ECA3544.
GeneIDi2882397.
KEGGieca:ECA3544.
PATRICi20482475. VBIPecAtr54885_3591.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX950851 Genomic DNA. Translation: CAG76442.1 .
RefSeqi WP_011095047.1. NC_004547.2.
YP_051632.1. NC_004547.2.

3D structure databases

ProteinModelPortali Q6D1A4.
SMRi Q6D1A4. Positions 1-463.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 218491.ECA3544.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAG76442 ; CAG76442 ; ECA3544 .
GeneIDi 2882397.
KEGGi eca:ECA3544.
PATRICi 20482475. VBIPecAtr54885_3591.

Phylogenomic databases

eggNOGi COG0007.
HOGENOMi HOG000290518.
KOi K02302.
OMAi LHQQLAW.
OrthoDBi EOG6DRPFR.

Enzyme and pathway databases

UniPathwayi UPA00148 ; UER00211 .
UPA00148 ; UER00222 .
UPA00262 ; UER00211 .
UPA00262 ; UER00222 .
UPA00262 ; UER00376 .
BioCyci PATR218491:GJNB-3618-MONOMER.

Family and domain databases

Gene3Di 1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPi MF_01646. Siroheme_synth.
InterProi IPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view ]
Pfami PF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMi SSF53790. SSF53790. 1 hit.
TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEi PS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SCRI 1043 / ATCC BAA-672.

Entry informationi

Entry nameiCYSG1_PECAS
AccessioniPrimary (citable) accession number: Q6D1A4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: August 16, 2004
Last modified: October 29, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3