Siroheme synthase 1 - Erwinia carotovora subsp. atroseptica

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Reviewed, UniProtKB/Swiss-Prot Q6D1A4 (CYSG1_ERWCT)

Last modified February 9, 2010. Version 38. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Siroheme synthase 1
Including the following 3 domains:
    1- Recommended name:
            Uroporphyrinogen-III C-methyltransferase
                Short name=Urogen III methylase
              EC=2.1.1.107
        Alternative name(s):
            SUMT
            Uroporphyrinogen III methylase
              Short name=UROM
    2- Recommended name:
            Precorrin-2 dehydrogenase
              EC=1.3.1.76
    3- Recommended name:
            Sirohydrochlorin ferrochelatase
              EC=4.99.1.4

Gene names

Name:	cysG1
Ordered Locus Names:	ECA3544

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

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Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into siroheme. This reaction consists of the NAD-dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme By similarity. HAMAP MF_01646
Catalytic activity	S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1. HAMAP MF_01646 S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2. HAMAP MF_01646 Precorrin-2 + NAD⁺ = sirohydrochlorin + NADH. HAMAP MF_01646 Siroheme + 2 H⁺ = sirohydrochlorin + Fe²⁺. HAMAP MF_01646
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP MF_01646 Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1. Porphyrin metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP MF_01646 Porphyrin metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1. HAMAP MF_01646 Porphyrin metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
Sequence similarities	Belongs to the precorrin methyltransferase family.

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Ontologies

Keywords
Biological process	Cobalamin biosynthesis Porphyrin biosynthesis
Ligand	NAD S-adenosyl-L-methionine
Molecular function	Lyase Methyltransferase Oxidoreductase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW siroheme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro precorrin-2 dehydrogenase activity Inferred from electronic annotation. Source: HAMAP sirohydrochlorin ferrochelatase activity Inferred from electronic annotation. Source: EC uroporphyrin-III C-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 480

480

Siroheme synthase 1 HAMAP MF_01646

PRO_0000330505

Regions

Region

224 – 464

241

Uroporphyrinogen-III C-methyltransferase HAMAP MF_01646

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Sequences

Sequence

Length

Mass (Da)

Tools

Q6D1A4-1 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 34E423B4160BB316
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FASTA

480

51,737

        10         20         30         40         50         60 
MNYLPIFADL RQRPVLVVGG GEVATRKIDL LQRAGAEVKI VALALAEPLA AQHQAGQVEW 

        70         80         90        100        110        120 
LAQSFTPELL SGVFLVIAAT DDAELNAAVF EAANQRHLLV NVVDDQPKCT FIFPSIVDRS 

       130        140        150        160        170        180 
PLVVAISSGG QAPVLARLLR EKLESLLPAS LGTMAEIAGS WRDRIKTRLH SMPARRRFWE 

       190        200        210        220        230        240 
RLFVGRFASL VSAGQLEQAE GELQQQLVNQ QDEQQLPAAA RGEVALVGAG PGDAGLLTLR 

       250        260        270        280        290        300 
GLQVMQQADV VLYDHLVSAD VLDLVRRDAE RICVGKRAST HSLPQGEINQ LLVKLAQEGK 

       310        320        330        340        350        360 
RVVRLKGGDP FIFGRGGEEL QAVAQAGITF QVVPGVTAAA GVTAYAGIPL THRDHAQSVI 

       370        380        390        400        410        420 
FITGHCRPDG DSLDWSTLAR GRQTLAIYMG TMKAAEISQQ LIAHGRSAQT PVAVISRGTR 

       430        440        450        460        470        480 
HDQQVQIGTL QELEHLARQA PTPALLVIGE VVDLHHQIAW FGQTTPTVPQ DSRPAVVNLA

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References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors."
Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z.

Toth I.K.
Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SCRI 1043 / ATCC BAA-672.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950851 Genomic DNA. Translation: CAG76442.1.
RefSeq	YP_051632.1.
3D structure databases
HSSP	HSSP built from PDB template 1PJS based on UniProtKB P25924.
SMR	Q6D1A4. Positions 1-463.
ModBase	Search...
Genome annotation databases
GeneID	2882397.
GenomeReviews	Gene locus ECA3544 in contig BX950851_GR.
KEGG	eca:ECA3544.
NMPDR	fig\|218491.3.peg.2190.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG730212.
OMA	DYAQSAV.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA3544-MONOMER.
Family and domain databases
HAMAP	MF_01646. Siroheme_synth. [Tree]
InterPro	IPR000878. 4pyrrol_Mease. IPR014777. 4pyrrole_Mease_sub1. IPR014776. 4pyrrole_Mease_sub2. IPR006366. CobA_cysG_C. IPR016040. NAD(P)-bd_dom. IPR019478. Sirohaem_synthase_dimer_dom. IPR006367. Sirohaem_synthase_N. IPR003043. Uropor_MeTrfase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1010.10. 4pyrrole_Mease_sub1. 1 hit. G3DSA:3.30.950.10. 4pyrrole_Mease_sub2. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF10414. CysG_dimeriser. 1 hit. PF00590. TP_methylase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01469. cobA_cysG_Cterm. 1 hit. TIGR01470. cysG_Nterm. 1 hit.
PROSITE	PS00839. SUMT_1. 1 hit. PS00840. SUMT_2. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

CYSG1_ERWCT

Accession

Primary (citable) accession number: Q6D1A4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	August 16, 2004
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents