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Q6D1A4 (CYSG1_PECAS) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Siroheme synthase 1

Including the following 3 domains:

  1. Uroporphyrinogen-III C-methyltransferase 1
    Short name=Urogen III methylase 1
    EC=2.1.1.107
    Alternative name(s):
    SUMT 1
    Uroporphyrinogen III methylase 1
    Short name=UROM 1
  2. Precorrin-2 dehydrogenase 1
    EC=1.3.1.76
  3. Sirohydrochlorin ferrochelatase 1
    EC=4.99.1.4
Gene names
Name:cysG1
Ordered Locus Names:ECA3544
OrganismPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica) [Complete proteome] [HAMAP]
Taxonomic identifier218491 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium

Protein attributes

Sequence length480 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme By similarity. HAMAP-Rule MF_01646

Catalytic activity

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1. HAMAP-Rule MF_01646

S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2. HAMAP-Rule MF_01646

Precorrin-2 + NAD+ = sirohydrochlorin + NADH. HAMAP-Rule MF_01646

Siroheme + 2 H+ = sirohydrochlorin + Fe2+. HAMAP-Rule MF_01646

Pathway

Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP-Rule MF_01646

Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.

Porphyrin-containing compound metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP-Rule MF_01646

Porphyrin-containing compound metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1.

Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.

Sequence similarities

In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.

In the C-terminal section; belongs to the precorrin methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 480480Siroheme synthase 1 HAMAP-Rule MF_01646
PRO_0000330505

Regions

Nucleotide binding22 – 232NAD By similarity
Nucleotide binding43 – 442NAD By similarity
Region1 – 203203precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase By similarity
Region222 – 480259Uroporphyrinogen-III C-methyltransferase By similarity
Region307 – 3093S-adenosyl-L-methionine binding By similarity
Region337 – 3382S-adenosyl-L-methionine binding By similarity

Sites

Active site2541Proton acceptor By similarity
Active site2761Proton donor By similarity
Binding site2311S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site3121S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site3891S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site4181S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity

Amino acid modifications

Modified residue1281Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6D1A4 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 34E423B4160BB316

FASTA48051,737
        10         20         30         40         50         60 
MNYLPIFADL RQRPVLVVGG GEVATRKIDL LQRAGAEVKI VALALAEPLA AQHQAGQVEW 

        70         80         90        100        110        120 
LAQSFTPELL SGVFLVIAAT DDAELNAAVF EAANQRHLLV NVVDDQPKCT FIFPSIVDRS 

       130        140        150        160        170        180 
PLVVAISSGG QAPVLARLLR EKLESLLPAS LGTMAEIAGS WRDRIKTRLH SMPARRRFWE 

       190        200        210        220        230        240 
RLFVGRFASL VSAGQLEQAE GELQQQLVNQ QDEQQLPAAA RGEVALVGAG PGDAGLLTLR 

       250        260        270        280        290        300 
GLQVMQQADV VLYDHLVSAD VLDLVRRDAE RICVGKRAST HSLPQGEINQ LLVKLAQEGK 

       310        320        330        340        350        360 
RVVRLKGGDP FIFGRGGEEL QAVAQAGITF QVVPGVTAAA GVTAYAGIPL THRDHAQSVI 

       370        380        390        400        410        420 
FITGHCRPDG DSLDWSTLAR GRQTLAIYMG TMKAAEISQQ LIAHGRSAQT PVAVISRGTR 

       430        440        450        460        470        480 
HDQQVQIGTL QELEHLARQA PTPALLVIGE VVDLHHQIAW FGQTTPTVPQ DSRPAVVNLA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX950851 Genomic DNA. Translation: CAG76442.1.
RefSeqYP_051632.1. NC_004547.2.

3D structure databases

ProteinModelPortalQ6D1A4.
SMRQ6D1A4. Positions 1-463.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING218491.ECA3544.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAG76442; CAG76442; ECA3544.
GeneID2882397.
KEGGeca:ECA3544.
PATRIC20482475. VBIPecAtr54885_3591.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0007.
HOGENOMHOG000290518.
KOK02302.
OMALHQQLAW.
OrthoDBEOG6DRPFR.

Enzyme and pathway databases

BioCycPATR218491:GJNB-3618-MONOMER.
UniPathwayUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Family and domain databases

Gene3D1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPMF_01646. Siroheme_synth.
InterProIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMSSF53790. SSF53790. 1 hit.
TIGRFAMsTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCYSG1_PECAS
AccessionPrimary (citable) accession number: Q6D1A4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: August 16, 2004
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways