Reviewed, UniProtKB/Swiss-Prot Q6D1A3 (CYSH_ERWCT)

Names and origin

Protein names

Recommended name:
    Phosphoadenosine phosphosulfate reductase
    EC=1.8.4.8
Alternative name(s):
    PAPS reductase, thioredoxin dependent
    PAdoPS reductase
    3'-phosphoadenylylsulfate reductase
    PAPS sulfotransferase

Gene names

Name:	cysH
Ordered Locus Names:	ECA3545

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite.
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin.
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Phosphoadenosine phosphosulfate reductase		PRO_1000008925

Sequences

	Sequence		Length	Mass (Da)
	Q6D1A3-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: EB26CDD1D820378F	FASTA	244	27,716
	10 20 30 40 50 60 MAEFNLEALN ALPKDEQVAA LAAVNGQLEQ LSAQERVSWA LENLPGDFVL SSSFGIQAAI 70 80 90 100 110 120 SLHLVTQQRP DIPVILTDTG YLFPETYQFI DALTEQLKLN LHVYRAAESP AWQESRYGKL 130 140 150 160 170 180 WDQGVEGIER YNLLNKVEPM NRALSELNAG TWFAGLRREQ SGSRGELPVL AIQRGVFKFL 190 200 210 220 230 240 PIIDWDNRTV YQYLKENGLS YHPLWDQGYL SVGDTHTTRK WEPGMSEEET RFFGLKRECG LHEG

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX950851 Genomic DNA. Translation: CAG76443.1.
RefSeq	YP_051633.1.
3D structure databases
SMR	Q6D1A3. Positions 2-230.
ModBase	Search...
Genome annotation databases
GeneID	2882116.
GenomeReviews	Gene locus ECA3545 in contig BX950851_GR.
KEGG	eca:ECA3545.
NMPDR	fig|218491.3.peg.2191.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6D1A3.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA3545-MON.
Family and domain databases
HAMAP	MF_00063. [Tree]
InterPro	IPR004511. CysH. IPR002500. PAPS_reduct. IPR011800. PAPS_reductase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR00434. cysH. 1 hit. TIGR02057. PAPS_reductase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_ERWCT

Accession

Primary (citable) accession number: Q6D1A3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 16, 2004
Last modified:	November 25, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)