Sulfite reductase [NADPH] hemoprotein beta-component - Erwinia carotovora subsp. atroseptica

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Reviewed, UniProtKB/Swiss-Prot Q6D1A2 (CYSI_ERWCT)

Last modified June 16, 2009. Version 33. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SIR-HP
      Short name=SIRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	ECA3546

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

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Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.
Catalytic activity	H₂S + 3 NADP⁺ + 3 H₂O = sulfite + 3 NADPH. HAMAP MF_01540
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Alpha(8)-beta₄. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

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Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP or NADPH binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 577

577

Sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540

PRO_0000199899

Sites

Metal binding

441

Iron-sulfur (4Fe-4S) By similarity

Metal binding

447

Iron-sulfur (4Fe-4S) By similarity

Metal binding

486

Iron-sulfur (4Fe-4S) By similarity

Metal binding

490

Iron (siroheme axial ligand) By similarity

Metal binding

490

Iron-sulfur (4Fe-4S) By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

Q6D1A2-1 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 5874EC77B2E84681
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FASTA

577

64,950

        10         20         30         40         50         60 
MSEKYVFSEK HPGPLVVEGK LTDAERMKTE SNFLRGTIAE DLNDGLTGGF KGDNFLLIRF 

        70         80         90        100        110        120 
HGMYQQDDRD IRAERAEQKL EPRHAMLLRC RLPGGVMTPQ QWLRIDKFAG ENTIYGSIRI 

       130        140        150        160        170        180 
TNRQTFQYHG ILKSNVKPVH QMLNSIGLDA LATANDMNRN VLCTSNPIES ELHQQAYEWA 

       190        200        210        220        230        240 
KTISEHLLPR TRAYAEIWMD QEKVATTDEE PILGSTYLPR KFKTTVVVPP QNDVDLHAND 

       250        260        270        280        290        300 
LNFVAIADNG RLVGFNVLVG GGLSIAHGDK ETYPRTASEL GYISIEHTLA IAEAVVTTQR 

       310        320        330        340        350        360 
DWGNRTNRKN AKTKYTLERV GVDNFKQEVE ARAGVKFEAV RPYEFTERGD RIGWVKGIDN 

       370        380        390        400        410        420 
KWHLTLFIEN GRILDYPGRP LKTGLAEIAK IHKGDFRLTA NQNLIVAGVP ARSKAKIDAL 

       430        440        450        460        470        480 
AREHGLIDDS VSEQRKNSMA CVSFPTCPLA MAEAERFLPE FVTKVEGIMQ QHGVGDEHIV 

       490        500        510        520        530        540 
LRVTGCPNGC GRSMLAEIGL VGKAMGRYNL HLGGNREGTR IPRMYRENIN ETEILAEIDR 

       550        560        570 
LVGLWAQDRL QNEGFGDFVI RTNIIRPVLD PARDFYD

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References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors."
Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z.

Toth I.K.
Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SCRI 1043 / ATCC BAA-672.

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Cross-references

Sequence databases
	BX950851 Genomic DNA. Translation: CAG76444.1.
RefSeq	YP_051634.1.
3D structure databases
SMR	Q6D1A2. Positions 87-577.
ModBase	Search...
Genome annotation databases
GeneID	2882117.
GenomeReviews	Gene locus ECA3546 in contig BX950851_GR.
KEGG	eca:ECA3546.
NMPDR	fig\|218491.3.peg.2192.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6D1A2.
OMA	Q6D1A2. AFQFHGI.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA3546-MON.
BRENDA	1.8.1.2. 281579.
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

CYSI_ERWCT

Accession

Primary (citable) accession number: Q6D1A2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	August 16, 2004
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents