Reviewed, UniProtKB/Swiss-Prot Q6D107 (AAS_ERWCT)

Names and origin

Protein names

Recommended name:
    Bifunctional protein aas
Including the following 2 domains:
    1- Recommended name:
            2-acylglycerophosphoethanolamine acyltransferase
              EC=2.3.1.40
        Alternative name(s):
            Acyl-[acyl-carrier-protein]--phospholipid O-acyltransferase
            2-acyl-GPE acyltransferase
    2- Recommended name:
            Acyl-[acyl-carrier-protein] synthetase
              EC=6.2.1.20
        Alternative name(s):
            Long-chain-fatty-acid--[acyl-carrier-protein] ligase
            Acyl-ACP synthetase

Gene names

Name:	aas
Ordered Locus Names:	ECA3641

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	738 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays a role in lysophospholipid acylation. Transfers fatty acids to the 1-position via an enzyme-bound acyl-ACP intermediate in the presence of ATP and magnesium. Its physiological function is to regenerate phosphatidylethanolamine from 2-acyl-glycero-3-phosphoethanolamine (2-acyl-GPE) formed by transacylation reactions or degradation by phospholipase A1 By similarity.
Catalytic activity	Acyl-[acyl-carrier-protein] + O-(2-acyl-sn-glycero-3-phospho)ethanolamine = [acyl-carrier-protein] + O-(1-beta-acyl-2-acyl-sn-glycero-3-phospho)ethanolamine. HAMAP MF_01162 ATP + an acid + [acyl-carrier-protein] = AMP + diphosphate + acyl-[acyl-carrier-protein]. HAMAP MF_01162
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	In the N-terminal section; belongs to the 2-acyl-GPE acetyltransferase family. In the C-terminal section; belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Acyltransferase Ligase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP long-chain-fatty-acid-[acyl-carrier-protein] ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 738	738	Bifunctional protein aas HAMAP MF_01162		PRO_0000193049
Regions
	Transmembrane	258 – 277	20	Potential
	Transmembrane	409 – 433	25	Potential
	Region	15 – 138	124	Acyltransferase HAMAP MF_01162
	Region	233 – 646	414	AMP-binding HAMAP MF_01162
Sites
	Active site	36	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6D107-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 814D20817360FCF8	FASTA	738	81,428
	10 20 30 40 50 60 MIHTLLRWVF QRLYRIRIEG DSSQFQQSKL LITPNHVSFL DGVLLALFLP IKPVFAVYSS 70 80 90 100 110 120 ISDRWFMRWL KPYIDFVPLD PTKPLAIKGL IKVIERGQPV VVFPEGRISV TGSLMKVYSG 130 140 150 160 170 180 AAFVAAKSGA TIIPVRIDGI ELSPFGRLAG VFKRRCLPQV TITYLPPTTL PMPEASSARA 190 200 210 220 230 240 RRALAGERLH QIMMKARMET RPQHTLYQAF LAARSRYGRH SASIADISFN EDSYQGLLKK 250 260 270 280 290 300 SLGVSRILQR FTRADEHVGM LLPNATITAA SILGASLRNR IPAMLNYTAG AKGLQSAMKA 310 320 330 340 350 360 AGIKTIVTSR QFLEKGKLTD LPKQVSEANW VYLEDLKDTV TLKDKLWILF HLLFPARAML 370 380 390 400 410 420 PQKPDDAAIV LFTSGSEGNP KGVVHSHDSL LANVEQIRTV ADFTPGDRFM SALPLFHAFG 430 440 450 460 470 480 LTVGLLTPLI TGARVFLYPS PLHYRIVPEL VYDQNCTVLF GTSTFLGNYA RFAHPYDFAR 490 500 510 520 530 540 LRYVVAGAEK LSETTRQVWQ DKFGIRILEG YGVTECAPVV AINVPMATKI HSVGLLLPEM 550 560 570 580 590 600 ESRLITVPGI TRGGRLQLRG PNIMKGYLRV ENPGVLETPA AENAEGELQQ GWYDTGDIVE 610 620 630 640 650 660 LDERGFCTII GRVKRFAKLA GEMVSLESVE QLAVKVSPEA QHAASAKSDS SKGEALVLFT 670 680 690 700 710 720 TDSQITRDVL LAQARSSGVP ELAVPRDIRY VKALPLLGSG KPDFVTLRQM AEEPNSEQSV 730 IEPSIVKQPI TNASEPSA

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX950851 Genomic DNA. Translation: CAG76539.1.
RefSeq	YP_051729.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2885271.
GenomeReviews	Gene locus ECA3641 in contig BX950851_GR.
KEGG	eca:ECA3641.
NMPDR	fig|218491.3.peg.2243.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6D107.
OMA	Q6D107. KGYLRVE.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA3641-MON.
BRENDA	2.3.1.40. 281579. 6.2.1.20. 281579.
Family and domain databases
HAMAP	MF_01162. [Tree]
InterPro	IPR002123. Acyltransferase. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. PF00501. AMP-binding. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AAS_ERWCT

Accession

Primary (citable) accession number: Q6D107

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	August 16, 2004
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)