Reviewed, UniProtKB/Swiss-Prot Q6D033 (SYK3_ERWCT)

Names and origin

Protein names

Recommended name:
    Putative lysyl-tRNA synthetase
    EC=6.1.1.6
Alternative name(s):
    Lysine--tRNA ligase
      Short name=LysRS
    GX

Gene names

Name:	poxA
Ordered Locus Names:	ECA3968

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Could be a lysyl-tRNA synthetase. HAMAP MF_00174
Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP MF_00174
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP lysine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Putative lysyl-tRNA synthetase HAMAP MF_00174		PRO_1000023622

Sequences

	Sequence		Length	Mass (Da)
	Q6D033-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 92734F2880F806D0	FASTA	325	36,782
	10 20 30 40 50 60 MSETTSWQPS ASVANLLKRA SIVAAVRRFF TDRGVLEVET PAMSQATVTD VFLYPFQTRF 70 80 90 100 110 120 VGPGAADGMT LYLMTSPEYH MKRLLAAGSG PIFQLCRSFR NEESGRHHNP EFTMLEWYRP 130 140 150 160 170 180 HYDMYRLMNE VDDLLQQVLD CESAEMLSYQ QAFLRHLEID PLSVDKAQLR EAAEKLGLGD 190 200 210 220 230 240 IACREDDRDS LVQMLFTFGV EPNIGRDKPA FVYHFPATQA SLAEISSEDH RVAERFEVYF 250 260 270 280 290 300 KGIELANGFR ELTDADEQRQ RFEQDNRKRA ARGLSQQPID ENLLAALKNG LPECSGVALG 310 320 VDRLIMLALK AEKLSDVIAF SVERA

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950851 Genomic DNA. Translation: CAG76865.1.
RefSeq	YP_052055.1.
3D structure databases
HSSP	HSSP built from PDB template 1BBW based on UniProtKB P0A8N3.
SMR	Q6D033. Positions 11-324.
ModBase	Search...
Genome annotation databases
GeneID	2885667.
GenomeReviews	Gene locus ECA3968 in contig BX950851_GR.
KEGG	eca:ECA3968.
NMPDR	fig|218491.3.peg.2682.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG631383.
OMA	PGNEAHL.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA3968-MONOMER.
BRENDA	6.1.1.6. 281579.
Family and domain databases
HAMAP	MF_00174. Put_Lys_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004525. Lys-tRNA-synth-rel. IPR018149. Lys-tRNA-synth_II_C. [Graphical view]
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. [Graphical view]
PRINTS	PR00982. TRNASYNTHLYS.
TIGRFAMs	TIGR00462. genX. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYK3_ERWCT

Accession

Primary (citable) accession number: Q6D033

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 16, 2004
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)