Q6D012 (ACEK_ERWCT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase kinase/phosphatase
Short name=IDH kinase/phosphatase
Short name=IDHK/P
EC=2.7.11.5
EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	ECA3989

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the AceK family.
Sequence caution	The sequence CAG76886.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: EC phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000057901
Regions
	Nucleotide binding	315 – 321	7	ATP By similarity
Sites
	Active site	371	1	By similarity
	Binding site	336	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6D012 [UniParc]. Last modified October 31, 2006. Version 2. Checksum: AD51A17FCFDE96D7	FASTA	591	69,173
	10 20 30 40 50 60 MTRDLEKLVA QTILQGFDAQ YGRFLEVTAG AQQRFEQADW PAVQQAMKQR IHLYDHHVGL 70 80 90 100 110 120 VVAQLRCITG IRCDDADFLA RMKHIYTGLL PDYPRFEIAE SFFNSVYCRL FNHRELAPDK 130 140 150 160 170 180 LFVFSSQPEK RFHEIPRPIA KTFVPTDGWQ RMLEKLLGDV PLRLPWEDLP RDIDYIVTYL 190 200 210 220 230 240 QSTFSAEQLE QATLQVANEL FYRNKAAWLA GKLSLPDGVF PFLLPIHHNE RGALFIDTCL 250 260 270 280 290 300 TAQADASMVF GFARSYFMVY APQPSALVAW LRDILPGKTT AELYLAIGCQ KHSKTEYYRE 310 320 330 340 350 360 YLHYIAESEE QFIIAPGVKG MVMLVFTLPS FDRVFKVIKD RFAPQKEVSA ERVMACYQLV 370 380 390 400 410 420 KEHDRVGRMA DTQEYENFVI DKHRISPELL DELWREVPEK LEDLGDQLVI RHLYMERRMT 430 440 450 460 470 480 PLNLYLEQAN AQQLHDVIEE YGNAIKQLAA ANIFPGDMLF KNFGVTRHGR VVFYDYDEIC 490 500 510 520 530 540 YMTEVNFRKI PPPRYLEDEL AAEPWYSVAP NDVFPEEFPH FLCSDRHIRT LFEEMHGDLF 550 560 570 580 590 CADYWRALQQ RIREGHIEDV YAYRRRKRFS QRAEPLYITT ENDSGLCRYP A

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950851 Genomic DNA. Translation: CAG76886.1. Different initiation.
RefSeq	YP_052076.1. NC_004547.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2884796.
GenomeReviews	Gene locus ECA3989 in contig BX950851_GR.
KEGG	eca:ECA3989.
NMPDR	fig|218491.3.peg.2703.
PATRIC	20483403. VBIPecAtr54885_4052.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG298593.
OMA	RMTPLNI.
ProtClustDB	PRK02946.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA3989-MONOMER.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
KO	K00906.
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_ERWCT

Accession

Primary (citable) accession number: Q6D012

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	October 31, 2006
Last modified:	January 25, 2012
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program