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Reviewed, UniProtKB/Swiss-Prot Q6CZT4 (PEL1_ERWCT)

Last modified January 19, 2010. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Pectate lyase 1
    EC=4.2.2.2
Alternative name(s):
    Pectate lyase I
      Short name=PEL I
    Pectate lyase A
      Short name=PLA
Gene names
Name: pel1
Synonyms: pelA
Ordered Locus Names: ECA4067
OrganismErwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]
Taxonomic identifier29471 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

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Protein attributes

Sequence length374 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Involved in maceration and soft-rotting of plant tissue.

Catalytic activity

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactor

Binds 1 calcium ion per subunit By similarity.

Pathway

Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.

Subcellular location

Secreted.

Sequence similarities

Belongs to the polysaccharide lyase 1 family. PLADES subfamily.

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Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionLyase
   PTMDisulfide bond
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

pectate lyase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Ref.1
Chain23 – 374352Pectate lyase 1
PRO_0000234448

Sites

Active site2391 Potential
Metal binding1501Calcium By similarity
Metal binding1521Calcium By similarity
Metal binding1871Calcium By similarity
Metal binding1911Calcium By similarity

Amino acid modifications

Disulfide bond93 ↔ 176 By similarity
Disulfide bond350 ↔ 373 By similarity

Experimental info

Sequence conflict131L → V in CAA57439. Ref.2
Sequence conflict51 – 6616VDIIE…SGKVV → INLIEEAQLDSKGKKL AA sequence Ref.1
Sequence conflict651V → A in CAA57439. Ref.2
Sequence conflict711F → Y in AAA24845. Ref.1
Sequence conflict711F → Y in CAA57439. Ref.2
Sequence conflict1121I → L in AAA24845. Ref.1
Sequence conflict1281V → L in AAA24845. Ref.1
Sequence conflict1281V → M in CAA57439. Ref.2
Sequence conflict1351V → I in CAA57439. Ref.2
Sequence conflict1541I → V in AAA24845. Ref.1
Sequence conflict1951A → G in AAA24845. Ref.1
Sequence conflict2051F → Y in AAA24845. Ref.1
Sequence conflict2051F → Y in CAA57439. Ref.2
Sequence conflict2281Y → H in AAA24845. Ref.1
Sequence conflict2331Y → H in AAA24845. Ref.1
Sequence conflict2521N → T in AAA24845. Ref.1
Sequence conflict2561D → G in CAA57439. Ref.2
Sequence conflict3141K → N in CAA57439. Ref.2
Sequence conflict3401S → P in AAA24845. Ref.1
Sequence conflict3401S → P in CAA57439. Ref.2
Sequence conflict3571N → S in CAA57439. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Q6CZT4-1 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: DDDA152B0F49CDDA

FASTA37440,178
        10         20         30         40         50         60 
MKYLLPSAAA GLLLLAAQPT MAANTGGYAT TDGGDVSGAV KKTARSLQEI VDIIEAAKKD 

        70         80         90        100        110        120 
SSGKVVKGGA FPLVITYNGN EDALIKAAEA NICGQWSKDP RGVEIKEFTK GITILGTNGS 

       130        140        150        160        170        180 
SANFGIWVVN SSNVVVRNMR FGYMPGGAKD GDAIRIDNSP NVWIDHNEIF AKNFECAGTP 

       190        200        210        220        230        240 
DNDTTFESAV DIKKASTNVT VSYNFIHGVK KVGLSGSSNT DTGRNLTYHH NIYSDVNSRL 

       250        260        270        280        290        300 
PLQRGGQVHA YNNLYDGIKS SGFNVRQKGI ALIESNWFEN ALNPVTARND DSNFGTWELR 

       310        320        330        340        350        360 
NNNITSPSDF AKYKITWGKP STPHINADDW KSTGKFPAVS YSYSPVSAQC VKDKLANYAG 

       370 
VGKNQAVLTA ANCK

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References

« Hide 'large scale' references
[1]"Characterization of the Erwinia carotovora pelA gene and its product pectate lyase A."
Lei S.-P., Lin H.-C., Wang S.-S., Wilcox G.
Gene 62:159-164(1988) [PubMed: 3371662] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 23-36.
Strain: EC.
[2]"Synergism between Erwinia pectate lyase isoenzymes that depolymerize both pectate and pectin."
Bartling S., Wegener C., Olsen O.
Microbiology 141:873-881(1995) [PubMed: 7773390] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C18.
[3]"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors."
Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. expand/collapse author list , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K.
Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SCRI 1043 / ATCC BAA-672.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M18859 Genomic DNA. Translation: AAA24845.1. Different initiation.
X81847 Genomic DNA. Translation: CAA57439.1.
BX950851 Genomic DNA. Translation: CAG76964.1.
PIRWZWCP1. JT0242.
RefSeqYP_052154.1.

3D structure databases

SMRQ6CZT4. Positions 22-373.
ModBaseSearch...

Genome annotation databases

GeneID2885350.
GenomeReviewsGene locus ECA4067 in contig BX950851_GR.
KEGGeca:ECA4067.
NMPDRfig|218491.3.peg.2898.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG401253.

Enzyme and pathway databases

BioCycECAR218491:ECA4067-MONOMER.
BRENDA4.2.2.2. 281579.

Family and domain databases

InterProIPR002022. Amb_allergen.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
Gene3DG3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
PfamPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
SMARTSM00656. Amb_all. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePEL1_ERWCT
AccessionPrimary (citable) accession number: Q6CZT4
Secondary accession number(s): P11430 expand/collapse secondary AC list , P16529, Q06113, Q47468
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: August 16, 2004
Last modified: January 19, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents