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Reviewed, UniProtKB/Swiss-Prot Q6CZT2 (PEL3_ERWCT)

Last modified November 3, 2009. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Pectate lyase 3
    EC=4.2.2.2
Alternative name(s):
    Pectate lyase III
      Short name=PEL III
    Pectate lyase C
      Short name=PLC
Gene names
Name: pel3
Synonyms: pelC, pelCI
Ordered Locus Names: ECA4069
OrganismErwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]
Taxonomic identifier29471 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

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Protein attributes

Sequence length374 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Involved in maceration and soft-rotting of plant tissue.

Catalytic activity

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactor

Binds 1 calcium ion per subunit By similarity.

Pathway

Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.

Subcellular location

Secreted.

Sequence similarities

Belongs to the polysaccharide lyase 1 family. PLADES subfamily.

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Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionLyase
   PTMDisulfide bond
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

pectate lyase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 By similarity
Chain23 – 374352Pectate lyase 3
PRO_0000234450

Sites

Active site2391 Potential
Metal binding1501Calcium By similarity
Metal binding1521Calcium By similarity
Metal binding1871Calcium By similarity
Metal binding1911Calcium By similarity

Amino acid modifications

Disulfide bond93 ↔ 176 By similarity
Disulfide bond350 ↔ 373 By similarity

Experimental info

Sequence conflict81T → A in CAA57441. Ref.1
Sequence conflict221A → V in CAA57441. Ref.1
Sequence conflict351N → D in CAA57441. Ref.1
Sequence conflict411N → K in CAA57441. Ref.1
Sequence conflict561E → A in CAA57441. Ref.1
Sequence conflict621K → N in CAA57441. Ref.1
Sequence conflict711Y → L in CAA57441. Ref.1
Sequence conflict741I → V in CAA57441. Ref.1
Sequence conflict77 – 782YN → FI in CAA57441. Ref.1
Sequence conflict901N → A in CAA57441. Ref.1
Sequence conflict1121V → I in CAA57441. Ref.1
Sequence conflict1151I → L in CAA57441. Ref.1
Sequence conflict134 – 1352VI → IV in CAA57441. Ref.1
Sequence conflict1771Q → A in CAA57441. Ref.1
Sequence conflict2011V → I in CAA57441. Ref.1
Sequence conflict3041I → V in CAA57441. Ref.1
Sequence conflict3231A → P in CAA57441. Ref.1
Sequence conflict3271A → S in CAA57441. Ref.1
Sequence conflict3321S → N in CAA57441. Ref.1
Sequence conflict3481P → A in CAA57441. Ref.1
Sequence conflict3631K → Q in CAA57441. Ref.1
Sequence conflict3661A → S in CAA57441. Ref.1
Sequence conflict3691T → S in CAA57441. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q6CZT2-1 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 9F404E0B3689724F

FASTA37440,682
        10         20         30         40         50         60 
MKYLLPSTAA GLLLLAAQPT MAANTGGYAT TDGGNVAGAV NKTARSMQDI IDIIEEAKLD 

        70         80         90        100        110        120 
SKGKKVKGGA YPLIITYNGN EDALIKAAEN NICGQWSKDA RGVEIKEFTK GVTIIGTNGS 

       130        140        150        160        170        180 
SANFGIWLTK SSDVIIRNMR FGYMPGGAQD GDAIRIDNTP NVWIDHNEIF AKNFECQGTK 

       190        200        210        220        230        240 
DGDTTFESAI DIKKASTNVT VSYNYIHGIK KVGLSGFSSS DTGRDLTYHH NIYDDVNARL 

       250        260        270        280        290        300 
PLQRGGQVHA YNNLYTGITS SGLNVRQKGI ALIERNWFEN AKNPVTSRYD GSNFGTWELR 

       310        320        330        340        350        360 
NNNIMSPADF AKYNITWDKD TKAYVNAEDW KSTGTFASVP YSYSPVSPQC VKDKLANYAG 

       370 
VNKNLAVLTA ANCN

« Hide

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References

« Hide 'large scale' references
[1]"Synergism between Erwinia pectate lyase isoenzymes that depolymerize both pectate and pectin."
Bartling S., Wegener C., Olsen O.
Microbiology 141:873-881(1995) [PubMed: 7773390] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C18.
[2]"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors."
Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. expand/collapse author list , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K.
Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SCRI 1043 / ATCC BAA-672.

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Cross-references

Sequence databases

X81847 Genomic DNA. Translation: CAA57441.1.
BX950851 Genomic DNA. Translation: CAG76966.1.
RefSeqYP_052156.1.

3D structure databases

HSSPHSSP built from PDB template 1AIR based on UniProtKB P11073.
ModBaseSearch...

Genome annotation databases

GeneID2885604.
GenomeReviewsGene locus ECA4069 in contig BX950851_GR.
KEGGeca:ECA4069.
NMPDRfig|218491.3.peg.2900.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ6CZT2.
OMATFESAID.

Enzyme and pathway databases

BioCycECAR218491:ECA4069-MON.
BRENDA4.2.2.2. 281579.

Family and domain databases

InterProIPR002022. Amb_allergen.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
[Graphical view]
Gene3DG3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
PfamPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
SMARTSM00656. Amb_all. 1 hit.
SM00710. PbH1. 5 hits.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePEL3_ERWCT
AccessionPrimary (citable) accession number: Q6CZT2
Secondary accession number(s): O31035 expand/collapse secondary AC list , P14006, P29171, Q47470
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: August 16, 2004
Last modified: November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents