Pectate lyase 3 precursor - Erwinia carotovora subsp. atroseptica

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Reviewed, UniProtKB/Swiss-Prot Q6CZT2 (PEL3_ERWCT)

Last modified January 19, 2010. Version 38. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Pectate lyase 3
    EC=4.2.2.2
Alternative name(s):
    Pectate lyase III
      Short name=PEL III
    Pectate lyase C
      Short name=PLC

Gene names

Name:	pel3
Synonyms:	pelC, pelCI
Ordered Locus Names:	ECA4069

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

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Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	Involved in maceration and soft-rotting of plant tissue.
Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Subcellular location	Secreted.
Sequence similarities	Belongs to the polysaccharide lyase 1 family. PLADES subfamily.

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Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 22

By similarity

Chain

23 – 374

352

Pectate lyase 3

PRO_0000234450

Sites

Active site

239

Potential

Metal binding

150

Calcium By similarity

Metal binding

152

Calcium By similarity

Metal binding

187

Calcium By similarity

Metal binding

191

Calcium By similarity

Amino acid modifications

Disulfide bond

93 ↔ 176

By similarity

Disulfide bond

350 ↔ 373

By similarity

Experimental info

Sequence conflict

T → A in CAA57441. Ref.1

Sequence conflict

A → V in CAA57441. Ref.1

Sequence conflict

N → D in CAA57441. Ref.1

Sequence conflict

N → K in CAA57441. Ref.1

Sequence conflict

E → A in CAA57441. Ref.1

Sequence conflict

K → N in CAA57441. Ref.1

Sequence conflict

Y → L in CAA57441. Ref.1

Sequence conflict

I → V in CAA57441. Ref.1

Sequence conflict

77 – 78

YN → FI in CAA57441. Ref.1

Sequence conflict

N → A in CAA57441. Ref.1

Sequence conflict

112

V → I in CAA57441. Ref.1

Sequence conflict

115

I → L in CAA57441. Ref.1

Sequence conflict

134 – 135

VI → IV in CAA57441. Ref.1

Sequence conflict

177

Q → A in CAA57441. Ref.1

Sequence conflict

201

V → I in CAA57441. Ref.1

Sequence conflict

304

I → V in CAA57441. Ref.1

Sequence conflict

323

A → P in CAA57441. Ref.1

Sequence conflict

327

A → S in CAA57441. Ref.1

Sequence conflict

332

S → N in CAA57441. Ref.1

Sequence conflict

348

P → A in CAA57441. Ref.1

Sequence conflict

363

K → Q in CAA57441. Ref.1

Sequence conflict

366

A → S in CAA57441. Ref.1

Sequence conflict

369

T → S in CAA57441. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

Q6CZT2-1 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 9F404E0B3689724F
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FASTA

374

40,682

        10         20         30         40         50         60 
MKYLLPSTAA GLLLLAAQPT MAANTGGYAT TDGGNVAGAV NKTARSMQDI IDIIEEAKLD 

        70         80         90        100        110        120 
SKGKKVKGGA YPLIITYNGN EDALIKAAEN NICGQWSKDA RGVEIKEFTK GVTIIGTNGS 

       130        140        150        160        170        180 
SANFGIWLTK SSDVIIRNMR FGYMPGGAQD GDAIRIDNTP NVWIDHNEIF AKNFECQGTK 

       190        200        210        220        230        240 
DGDTTFESAI DIKKASTNVT VSYNYIHGIK KVGLSGFSSS DTGRDLTYHH NIYDDVNARL 

       250        260        270        280        290        300 
PLQRGGQVHA YNNLYTGITS SGLNVRQKGI ALIERNWFEN AKNPVTSRYD GSNFGTWELR 

       310        320        330        340        350        360 
NNNIMSPADF AKYNITWDKD TKAYVNAEDW KSTGTFASVP YSYSPVSPQC VKDKLANYAG 

       370 
VNKNLAVLTA ANCN

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Synergism between Erwinia pectate lyase isoenzymes that depolymerize both pectate and pectin." Bartling S., Wegener C., Olsen O. Microbiology 141:873-881(1995) [PubMed: 7773390] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C18.
[2]	"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	X81847 Genomic DNA. Translation: CAA57441.1. BX950851 Genomic DNA. Translation: CAG76966.1.
RefSeq	YP_052156.1.
3D structure databases
SMR	Q6CZT2. Positions 22-373.
ModBase	Search...
Genome annotation databases
GeneID	2885604.
GenomeReviews	Gene locus ECA4069 in contig BX950851_GR.
KEGG	eca:ECA4069.
NMPDR	fig\|218491.3.peg.2900.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG401253.
OMA	TFESAID.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA4069-MONOMER.
BRENDA	4.2.2.2. 281579.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
SMART	SM00656. Amb_all. 1 hit. SM00710. PbH1. 5 hits. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

PEL3_ERWCT

Accession

Primary (citable) accession number: Q6CZT2
Secondary accession number(s): O31035

Q47470

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	August 16, 2004
Last modified:	January 19, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents