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Q6CZK0 (GLGB_PECAS) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:ECA4151
OrganismPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica) [Complete proteome] [HAMAP]
Taxonomic identifier218491 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium

Protein attributes

Sequence length725 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7257251,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188706

Sites

Active site4031Nucleophile By similarity
Active site4561Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6CZK0 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 4F94B0ADFB303336

FASTA72583,656
        10         20         30         40         50         60 
MSAFSEAINS LFSGHYADPF SLLGMHHSSK GLEVRALLPD ADAAWVVDAS NGRKIVELER 

        70         80         90        100        110        120 
VDGRGFFCGL VPNRKHDFHY QLAVTWREET WVIEDPYRFG PLLQDMDIWL LAEGTHLRPY 

       130        140        150        160        170        180 
ERLGAHLETL DGVEGTRFAV WAPNAQRVSV VGQFNFWDGR RHPMRLRKEN GIWELFLPDV 

       190        200        210        220        230        240 
KAGQLYKYEM IDSHGSVRLK ADPYAFEAQM RPDTASLITP LPEKVPTNEA RREANSLRSP 

       250        260        270        280        290        300 
ISIYEVHLGS WRRHTDNNFW LSYQELANQL IDYVQYMGFT HVELMPINEH PFDGSWGYQP 

       310        320        330        340        350        360 
LGLYAPTRRF GTASDFRAFV DALHGAGINV LLDWVPGHFP GDEYGLAQFD GTALYEYSDP 

       370        380        390        400        410        420 
REGYHQDWNT LIYNYGRHEV RNYLAGNALF WMERYGIDGL RVDAVASMIY RDYSRSEGEW 

       430        440        450        460        470        480 
VPNHYGGKEN LEAIAFLRYT NHTLGHAAPA AITLAEESTD YPGVTLPPDC NGLGFHYKWN 

       490        500        510        520        530        540 
MGWMHDTLTY MQLDPVHRKH HHDLLTFGML YAYSENFVLP LSHDEVVHGK RSLLDRMPGD 

       550        560        570        580        590        600 
VWQKFANLRA YYGFMWAYPG KKLLFMGCEF AQGREWNHDA SLDWHLLDEP EGWHRGVQAL 

       610        620        630        640        650        660 
VRDLNHYYRQ QPSLYQLDFQ PQGFEWLVVD DRENSVFAFI RRDEQGNEVL VVSNFTPVPR 

       670        680        690        700        710        720 
YGYRIGINQP GGWREVMNTD SVHYNGSDQG NVGTIYSEEW GSHQRQHSLV LTIPPLATLY 


LVKEA 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX950851 Genomic DNA. Translation: CAG77048.1.
RefSeqYP_052238.1. NC_004547.2.

3D structure databases

ProteinModelPortalQ6CZK0.
SMRQ6CZK0. Positions 115-725.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING218491.ECA4151.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAG77048; CAG77048; ECA4151.
GeneID2882436.
KEGGeca:ECA4151.
PATRIC20483757. VBIPecAtr54885_4224.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycPATR218491:GJNB-4235-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_PECAS
AccessionPrimary (citable) accession number: Q6CZK0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: August 16, 2004
Last modified: June 11, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries