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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei403 – 4031NucleophileUniRule annotation
Active sitei456 – 4561Proton donorUniRule annotation

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciPATR218491:GJNB-4235-MONOMER.
UniPathwayiUPA00164.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:ECA4151
OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Taxonomic identifieri218491 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium
ProteomesiUP000007966 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7257251,4-alpha-glucan branching enzyme GlgBPRO_0000188706Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi218491.ECA4151.

Structurei

3D structure databases

ProteinModelPortaliQ6CZK0.
SMRiQ6CZK0. Positions 115-725.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiYWFEQFH.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6CZK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAFSEAINS LFSGHYADPF SLLGMHHSSK GLEVRALLPD ADAAWVVDAS
60 70 80 90 100
NGRKIVELER VDGRGFFCGL VPNRKHDFHY QLAVTWREET WVIEDPYRFG
110 120 130 140 150
PLLQDMDIWL LAEGTHLRPY ERLGAHLETL DGVEGTRFAV WAPNAQRVSV
160 170 180 190 200
VGQFNFWDGR RHPMRLRKEN GIWELFLPDV KAGQLYKYEM IDSHGSVRLK
210 220 230 240 250
ADPYAFEAQM RPDTASLITP LPEKVPTNEA RREANSLRSP ISIYEVHLGS
260 270 280 290 300
WRRHTDNNFW LSYQELANQL IDYVQYMGFT HVELMPINEH PFDGSWGYQP
310 320 330 340 350
LGLYAPTRRF GTASDFRAFV DALHGAGINV LLDWVPGHFP GDEYGLAQFD
360 370 380 390 400
GTALYEYSDP REGYHQDWNT LIYNYGRHEV RNYLAGNALF WMERYGIDGL
410 420 430 440 450
RVDAVASMIY RDYSRSEGEW VPNHYGGKEN LEAIAFLRYT NHTLGHAAPA
460 470 480 490 500
AITLAEESTD YPGVTLPPDC NGLGFHYKWN MGWMHDTLTY MQLDPVHRKH
510 520 530 540 550
HHDLLTFGML YAYSENFVLP LSHDEVVHGK RSLLDRMPGD VWQKFANLRA
560 570 580 590 600
YYGFMWAYPG KKLLFMGCEF AQGREWNHDA SLDWHLLDEP EGWHRGVQAL
610 620 630 640 650
VRDLNHYYRQ QPSLYQLDFQ PQGFEWLVVD DRENSVFAFI RRDEQGNEVL
660 670 680 690 700
VVSNFTPVPR YGYRIGINQP GGWREVMNTD SVHYNGSDQG NVGTIYSEEW
710 720
GSHQRQHSLV LTIPPLATLY LVKEA
Length:725
Mass (Da):83,656
Last modified:August 16, 2004 - v1
Checksum:i4F94B0ADFB303336
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950851 Genomic DNA. Translation: CAG77048.1.
RefSeqiWP_011095622.1. NC_004547.2.
YP_052238.1. NC_004547.2.

Genome annotation databases

EnsemblBacteriaiCAG77048; CAG77048; ECA4151.
GeneIDi2882436.
KEGGieca:ECA4151.
PATRICi20483757. VBIPecAtr54885_4224.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950851 Genomic DNA. Translation: CAG77048.1.
RefSeqiWP_011095622.1. NC_004547.2.
YP_052238.1. NC_004547.2.

3D structure databases

ProteinModelPortaliQ6CZK0.
SMRiQ6CZK0. Positions 115-725.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi218491.ECA4151.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG77048; CAG77048; ECA4151.
GeneIDi2882436.
KEGGieca:ECA4151.
PATRICi20483757. VBIPecAtr54885_4224.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiYWFEQFH.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciPATR218491:GJNB-4235-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SCRI 1043 / ATCC BAA-672.

Entry informationi

Entry nameiGLGB_PECAS
AccessioniPrimary (citable) accession number: Q6CZK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: August 16, 2004
Last modified: April 1, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.