Q6CZD8 (GPPA_ERWCT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase
EC=3.6.1.40

Alternative name(s):
Guanosine pentaphosphate phosphohydrolase
pppGpp-5'-phosphohydrolase

Gene names

Name:	gppA
Ordered Locus Names:	ECA4214

Organism

Erwinia carotovora subsp. atroseptica (strain SCRI 1043 / ATCC BAA-672) (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

218491 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium › Erwinia carotovora subsp. atroseptica

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the "stringent response", an adaptive process that allows bacteria to respond to amino acid starvation, resulting in the coordinated regulation of numerous cellular activities By similarity. HAMAP-Rule MF_01550
Catalytic activity	Guanosine 5'-triphosphate,3'-diphosphate + H2O = guanosine 3',5'-bis(diphosphate) + phosphate. HAMAP-Rule MF_01550
Pathway	Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2. HAMAP-Rule MF_01550
Sequence similarities	Belongs to the GppA/Ppx family. GppA subfamily.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	guanosine pentaphosphate catabolic process Inferred from electronic annotation. Source: InterPro guanosine tetraphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway polyphosphate catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	exopolyphosphatase activity Inferred from electronic annotation. Source: InterPro guanosine-5'-triphosphate,3'-diphosphate diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase HAMAP-Rule MF_01550		PRO_0000194279

Sequences

	Sequence		Length	Mass (Da)
	Q6CZD8 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 7AC67D354A932836	FASTA	498	55,719
	10 20 30 40 50 60 MLSSSSLYAA IDLGSNSFHM LVTRETAGSI QTLAKIKRKV RLAAGLDKQN RLSQEAMQRG 70 80 90 100 110 120 WQCLQLFSER LQDIPQDQVR VVATATLRLA TNADEFLQRA QEILGLPIQV ISGEEEARLI 130 140 150 160 170 180 YQGVAHTTGG PDARLVVDIG GGSTELATGI GAKTTQLISL PMGCVTWLDR YFSDRNLEAG 190 200 210 220 230 240 NFERAENAAR EMLRPVAASL REQGWQICVG ASGTVQALQE IMVAQGMDEY ITLPKLRQLK 250 260 270 280 290 300 EHAIQCDKLE ELEIDGLTLE RALVFPSGLA ILLAIFQELD IKTMTLAGGA LREGLVYGML 310 320 330 340 350 360 HLPVDQDIRH RTLATLQRRY LLDTEQAKRV STLADNFLQQ VARDWQLDSR CRELLRSACM 370 380 390 400 410 420 VHEIGLSIDF RQSPQHAAYL IRHSDLPGFT PAQKKLLATL LQNQINPIDL MPLSQQNALP 430 440 450 460 470 480 VNQAQHLCRL LRLAIIFASR RRDDTLPAVR LRVEGEALRL ILPAGWLAQH PLRAEMLEQE 490 SRWQSYVHWP LMLEEAPA

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950851 Genomic DNA. Translation: CAG77111.1.
RefSeq	YP_052301.1. NC_004547.2.
3D structure databases
ProteinModelPortal	Q6CZD8.
ModBase	Search...
Protein-protein interaction databases
STRING	218491.ECA4214.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAG77111; CAG77111; ECA4214.
GeneID	2885504.
KEGG	eca:ECA4214.
PATRIC	20483897. VBIPecAtr54885_4290.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0248.
HOGENOM	HOG000258672.
KO	K01524.
OMA	PPRVAEH.
ProtClustDB	PRK11031.
Enzyme and pathway databases
BioCyc	PATR218491:GJNB-4303-MONOMER.
UniPathway	UPA00908; UER00884.
Family and domain databases
HAMAP	MF_01550. GppA.
InterPro	IPR022371. Exopolyphosphatase. IPR023709. Guo-5TP_3DP_PyrP. IPR003695. Ppx_GppA. [Graphical view]
Pfam	PF02541. Ppx-GppA. 1 hit. [Graphical view]
TIGRFAMs	TIGR03706. exo_poly_only. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GPPA_ERWCT

Accession

Primary (citable) accession number: Q6CZD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	August 16, 2004
Last modified:	May 29, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program