Reviewed, UniProtKB/Swiss-Prot Q6CZB1 (STHA_ERWCT)

Names and origin

Protein names

Recommended name:
    Soluble pyridine nucleotide transhydrogenase
      Short name=STH
    EC=1.6.1.1
Alternative name(s):
    NAD(P)(+) transhydrogenase [B-specific]

Gene names

Name:	sthA
Synonyms:	udhA
Ordered Locus Names:	ECA4242

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation By similarity.
Catalytic activity	NADPH + NAD+ = NADP+ + NADH. HAMAP MF_00247
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NADP metabolic process Inferred from electronic annotation. Source: HAMAP cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NAD(P)+ transhydrogenase (B-specific) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	Soluble pyridine nucleotide transhydrogenase HAMAP MF_00247		PRO_0000260233
Regions
	Nucleotide binding	38 – 47	10	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6CZB1-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 045923BF1E8CFDD7	FASTA	468	51,554
	10 20 30 40 50 60 MTLEHQFDYD AIIIGSGPGG EGAAMGLAKH GAKIAVIERH YNVGGGCTHW GTIPSKALRH 70 80 90 100 110 120 AVSRIIEFNQ NPLYSDNSRI IRSSFSDILR HADSVIGQQT RMRQGFYERN QCELFSGEAS 130 140 150 160 170 180 FIDAHTIAVH YPDNTHETLT AANIIIATGS RPYHPAEVDF NHPRIYDSDS ILQLDHEPQH 190 200 210 220 230 240 VIIYGAGVIG CEYASIFRGL SVKVDLINTR DRLLAFLDQE MSDALSYHFW NNGVVIRHNE 250 260 270 280 290 300 EFDSIEGLSD GVIVNLKSGK KMKADCLLYA NGRTGNTETL GLENIGLSTD SRGQLKVNSM 310 320 330 340 350 360 YQTALAHIYA IGDVIGYPSL ASAAYDQGRL AAQAIIKGDA SAHLIEDIPT GIYTIPEISS 370 380 390 400 410 420 VGKTEQELTA MKVPYEVGRA QFKHLARAQI VGMNVGSLKI LFHRETKQIL GIHCFGERAA 430 440 450 460 EIIHIGQAIM EQKGEGNTIE YFVNTTFNYP TMAEAYRVAA LNGLNRLF

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX950851 Genomic DNA. Translation: CAG77139.1.
RefSeq	YP_052329.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2884342.
GenomeReviews	Gene locus ECA4242 in contig BX950851_GR.
KEGG	eca:ECA4242.
NMPDR	fig|218491.3.peg.487.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6CZB1.
OMA	Q6CZB1. GEGNTIE.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA4242-MON.
BRENDA	1.6.1.1. 281579.
Family and domain databases
HAMAP	MF_00247. [Tree]
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR000815. Hg_reductase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

STHA_ERWCT

Accession

Primary (citable) accession number: Q6CZB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	August 16, 2004
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)