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Q6CY47 (TRMB_KLULA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:TRM8
Ordered Locus Names:KLLA0A03245g
OrganismKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) [Complete proteome]
Taxonomic identifier284590 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces

Protein attributes

Sequence length278 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subunit structure

Forms a complex with TRM82 By similarity. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 278278tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370597

Regions

Region118 – 1192S-adenosyl-L-methionine binding By similarity
Region153 – 1542S-adenosyl-L-methionine binding By similarity
Region251 – 2533S-adenosyl-L-methionine binding By similarity

Sites

Active site1761 By similarity
Binding site951S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1731S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6CY47 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: D47A49F22D974F64

FASTA27832,701
        10         20         30         40         50         60 
MSFSTRRQAY RAEKQDHRKE LKHVKIDESV IEKVDKLSLP KKKFYRQRAH SNPFSDHQLE 

        70         80         90        100        110        120 
YPISPAHMDW SKLYPHFYDS EKKKMTKDVT IADIGCGYGG LMIDLSPEFP DEMILGMEIR 

       130        140        150        160        170        180 
VQVTNYVEDR IIALRTNHAK ENGYQNINVL RGNAMKFLPN FFNKGQLSKI FFCFPDPHFK 

       190        200        210        220        230        240 
QRKHKARIVT DTLLSEYAYV LKEGGIIYTI TDVLDLHEWM VKHLEEHPLF ERLSEEWEAQ 

       250        260        270 
DKCVSIMRNA TEEGKKVERN KGDKYIACFV RLPTPDII 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR382121 Genomic DNA. Translation: CAH02730.1.
RefSeqXP_451142.1. XM_451142.1.

3D structure databases

ProteinModelPortalQ6CY47.
SMRQ6CY47. Positions 52-278.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING28985.Q6CY47.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2896541.
KEGGkla:KLLA0A03245g.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000260965.
KOK03439.
OMARAHSNPI.
OrthoDBEOG708W9T.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_KLULA
AccessionPrimary (citable) accession number: Q6CY47
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: August 16, 2004
Last modified: April 16, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways