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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

TRM8

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei95S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei173S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei176UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:TRM8UniRule annotation
Ordered Locus Names:KLLA0A03245g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705971 – 278tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST278

Interactioni

Subunit structurei

Forms a complex with TRM82.UniRule annotation

Protein-protein interaction databases

STRINGi284590.XP_451142.1

Structurei

3D structure databases

ProteinModelPortaliQ6CY47
SMRiQ6CY47
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni118 – 119S-adenosyl-L-methionine bindingUniRule annotation2
Regioni153 – 154S-adenosyl-L-methionine bindingUniRule annotation2
Regioni251 – 253S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3115 Eukaryota
COG0220 LUCA
HOGENOMiHOG000260965
InParanoidiQ6CY47
KOiK03439
OMAiIGMEIRV
OrthoDBiEOG092C0GMH

Family and domain databases

HAMAPiMF_03055 tRNA_methyltr_TrmB_euk, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR025763 Trm8_euk
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
PTHR23417:SF16 PTHR23417:SF16, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q6CY47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFSTRRQAY RAEKQDHRKE LKHVKIDESV IEKVDKLSLP KKKFYRQRAH
60 70 80 90 100
SNPFSDHQLE YPISPAHMDW SKLYPHFYDS EKKKMTKDVT IADIGCGYGG
110 120 130 140 150
LMIDLSPEFP DEMILGMEIR VQVTNYVEDR IIALRTNHAK ENGYQNINVL
160 170 180 190 200
RGNAMKFLPN FFNKGQLSKI FFCFPDPHFK QRKHKARIVT DTLLSEYAYV
210 220 230 240 250
LKEGGIIYTI TDVLDLHEWM VKHLEEHPLF ERLSEEWEAQ DKCVSIMRNA
260 270
TEEGKKVERN KGDKYIACFV RLPTPDII
Length:278
Mass (Da):32,701
Last modified:August 16, 2004 - v1
Checksum:iD47A49F22D974F64
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382121 Genomic DNA Translation: CAH02730.1
RefSeqiXP_451142.1, XM_451142.1

Genome annotation databases

EnsemblFungiiCAH02730; CAH02730; KLLA0_A03245g
GeneIDi2896541
KEGGikla:KLLA0A03245g

Entry informationi

Entry nameiTRMB_KLULA
AccessioniPrimary (citable) accession number: Q6CY47
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: August 16, 2004
Last modified: January 31, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome