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Q6CY47

- TRMB_KLULA

UniProt

Q6CY47 - TRMB_KLULA

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene
TRM8, KLLA0A03245g
Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei95 – 951S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei173 – 1731S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Active sitei176 – 1761 By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:TRM8
Ordered Locus Names:KLLA0A03245g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598: Chromosome A

Subcellular locationi

Nucleus By similarity UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 278278tRNA (guanine-N(7)-)-methyltransferaseUniRule annotationPRO_0000370597Add
BLAST

Interactioni

Subunit structurei

Forms a complex with TRM82 By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi28985.Q6CY47.

Structurei

3D structure databases

ProteinModelPortaliQ6CY47.
SMRiQ6CY47. Positions 52-278.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni118 – 1192S-adenosyl-L-methionine binding By similarity
Regioni153 – 1542S-adenosyl-L-methionine binding By similarity
Regioni251 – 2533S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000260965.
KOiK03439.
OMAiRAHSNPI.
OrthoDBiEOG708W9T.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6CY47-1 [UniParc]FASTAAdd to Basket

« Hide

MSFSTRRQAY RAEKQDHRKE LKHVKIDESV IEKVDKLSLP KKKFYRQRAH    50
SNPFSDHQLE YPISPAHMDW SKLYPHFYDS EKKKMTKDVT IADIGCGYGG 100
LMIDLSPEFP DEMILGMEIR VQVTNYVEDR IIALRTNHAK ENGYQNINVL 150
RGNAMKFLPN FFNKGQLSKI FFCFPDPHFK QRKHKARIVT DTLLSEYAYV 200
LKEGGIIYTI TDVLDLHEWM VKHLEEHPLF ERLSEEWEAQ DKCVSIMRNA 250
TEEGKKVERN KGDKYIACFV RLPTPDII 278
Length:278
Mass (Da):32,701
Last modified:August 16, 2004 - v1
Checksum:iD47A49F22D974F64
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR382121 Genomic DNA. Translation: CAH02730.1.
RefSeqiXP_451142.1. XM_451142.1.

Genome annotation databases

GeneIDi2896541.
KEGGikla:KLLA0A03245g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR382121 Genomic DNA. Translation: CAH02730.1 .
RefSeqi XP_451142.1. XM_451142.1.

3D structure databases

ProteinModelPortali Q6CY47.
SMRi Q6CY47. Positions 52-278.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 28985.Q6CY47.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2896541.
KEGGi kla:KLLA0A03245g.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000260965.
KOi K03439.
OMAi RAHSNPI.
OrthoDBi EOG708W9T.

Enzyme and pathway databases

UniPathwayi UPA00989 .

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Entry informationi

Entry nameiTRMB_KLULA
AccessioniPrimary (citable) accession number: Q6CY47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: August 16, 2004
Last modified: June 11, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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