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Protein

Histone-lysine N-methyltransferase, H3 lysine-79 specific

Gene

DOT1

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].PROSITE-ProRule annotation

Enzyme regulationi

Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei411 – 4111S-adenosyl-L-methioninePROSITE-ProRule annotation

GO - Molecular functioni

  1. histone methyltransferase activity (H3-K79 specific) Source: InterPro

GO - Biological processi

  1. chromatin silencing at telomere Source: InterPro
  2. DNA damage checkpoint Source: InterPro
  3. DNA repair Source: InterPro
  4. regulation of cell cycle Source: InterPro
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase, H3 lysine-79 specific (EC:2.1.1.43)
Alternative name(s):
Histone H3-K79 methyltransferase
Short name:
H3-K79-HMTase
Gene namesi
Name:DOT1
Ordered Locus Names:KLLA0B01287g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598 Componenti: Chromosome B

Subcellular locationi

  1. Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 572572Histone-lysine N-methyltransferase, H3 lysine-79 specificPRO_0000270614Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi28985.Q6CWV1.

Structurei

3D structure databases

ProteinModelPortaliQ6CWV1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini245 – 558314DOT1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni361 – 3644S-adenosyl-L-methionine bindingPROSITE-ProRule annotation
Regioni384 – 39310S-adenosyl-L-methionine bindingPROSITE-ProRule annotation
Regioni448 – 4492S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.PROSITE-ProRule annotation
Contains 1 DOT1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG294902.
HOGENOMiHOG000112251.
InParanoidiQ6CWV1.
KOiK11427.
OMAiNFVYGEL.
OrthoDBiEOG7KH9VN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR021162. Dot1.
IPR025789. DOT1_dom.
IPR013110. DOT1_dom_Pfam.
IPR030445. H3-K79_meTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR21451. PTHR21451. 1 hit.
PfamiPF08123. DOT1. 1 hit.
[Graphical view]
PIRSFiPIRSF017570. Histone_H3-K79_MeTrfase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51569. DOT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6CWV1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTEATSSDA SVFSNMHSVD STRDNTRDNT RDNTPMNEGD TQGKRMTKST
60 70 80 90 100
MALIEESMKY DMRSEYFLPM TFLRERRKPA RVESDEEDKL AEVKSKRRVS
110 120 130 140 150
KKDQVKADIH STKIKKTGRP SNVKAGAKNT SKVTKQRSTK SQVSGKKRAR
160 170 180 190 200
SISDGKVSSI DASRSLKASK KKHSSHADSL FLSNIEENVN PRPSFFNTTL
210 220 230 240 250
IANPSSFTNK LTSSKMFVDD EEVTSLKGMK YILFPGVEEE YLICSKVQKF
260 270 280 290 300
GYNPLPEIGQ SIESAVLLYF PNTYKTKGQE LVRSLNDAFE RSDDQSFEKI
310 320 330 340 350
VLKYNDLIST IPRDLIVKNL SSNPDVPVSF VHFLLHTCYT RAIYPHARKL
360 370 380 390 400
KKYTSFSNFV YGELMPDFLT IVFKKCGLNS NSIFMDLGSG VGNCVIQASL
410 420 430 440 450
EFGCKLSFGC EIMDSASDMA ELQLKELKSR CDLWGINLPP IDFSLRKSFV
460 470 480 490 500
DNERVRELIP QCDVILINNF IFDAPLNKEV EKVVQGLKAG SKIISLKSIR
510 520 530 540 550
PPGYSINYDD MDNIFNRLHV ESFKLPENSV SWTYRSVGDY YISTVLDAID
560 570
ESIFCPPILG RIRKKESIKY TR
Length:572
Mass (Da):64,796
Last modified:August 16, 2004 - v1
Checksum:iF830A57170781FF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382122 Genomic DNA. Translation: CAH01981.1.
RefSeqiXP_451588.1. XM_451588.1.

Genome annotation databases

GeneIDi2896923.
KEGGikla:KLLA0B01287g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382122 Genomic DNA. Translation: CAH01981.1.
RefSeqiXP_451588.1. XM_451588.1.

3D structure databases

ProteinModelPortaliQ6CWV1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi28985.Q6CWV1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2896923.
KEGGikla:KLLA0B01287g.

Phylogenomic databases

eggNOGiNOG294902.
HOGENOMiHOG000112251.
InParanoidiQ6CWV1.
KOiK11427.
OMAiNFVYGEL.
OrthoDBiEOG7KH9VN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR021162. Dot1.
IPR025789. DOT1_dom.
IPR013110. DOT1_dom_Pfam.
IPR030445. H3-K79_meTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR21451. PTHR21451. 1 hit.
PfamiPF08123. DOT1. 1 hit.
[Graphical view]
PIRSFiPIRSF017570. Histone_H3-K79_MeTrfase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51569. DOT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDOT1_KLULA
AccessioniPrimary (citable) accession number: Q6CWV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: August 16, 2004
Last modified: April 1, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.