Reviewed, UniProtKB/Swiss-Prot Q6CWV1 (DOT1_KLULA)

Names and origin

Protein names

Recommended name:
    Histone-lysine N-methyltransferase, H3 lysine-79 specific
    EC=2.1.1.43
Alternative name(s):
    Histone H3-K79 methyltransferase
    H3-K79-HMTase

Gene names

Name:	DOT1
Ordered Locus Names:	KLLA0B01287g

Organism

Kluyveromyces lactis (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

28985 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones By similarity.
Catalytic activity	S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.
Enzyme regulation	Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3 By similarity.
Subcellular location	Nucleus By similarity.
Miscellaneous	In contrast to other lysine histone methyltransferase, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.
Sequence similarities	Belongs to the DOT1 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Histone-lysine N-methyltransferase, H3 lysine-79 specific		PRO_0000270614
Regions
	Region	386 – 388	3	S-adenosyl-L-methionine binding By similarity
	Region	448 – 449	2	S-adenosyl-L-methionine binding By similarity
	Motif	382 – 393	12	SAM-binding motif 1 By similarity
	Motif	463 – 472	10	SAM-binding motif 2 By similarity
Sites
	Binding site	364	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	411	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6CWV1-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: F830A57170781FF6	FASTA	572	64,796
	10 20 30 40 50 60 MSTEATSSDA SVFSNMHSVD STRDNTRDNT RDNTPMNEGD TQGKRMTKST MALIEESMKY 70 80 90 100 110 120 DMRSEYFLPM TFLRERRKPA RVESDEEDKL AEVKSKRRVS KKDQVKADIH STKIKKTGRP 130 140 150 160 170 180 SNVKAGAKNT SKVTKQRSTK SQVSGKKRAR SISDGKVSSI DASRSLKASK KKHSSHADSL 190 200 210 220 230 240 FLSNIEENVN PRPSFFNTTL IANPSSFTNK LTSSKMFVDD EEVTSLKGMK YILFPGVEEE 250 260 270 280 290 300 YLICSKVQKF GYNPLPEIGQ SIESAVLLYF PNTYKTKGQE LVRSLNDAFE RSDDQSFEKI 310 320 330 340 350 360 VLKYNDLIST IPRDLIVKNL SSNPDVPVSF VHFLLHTCYT RAIYPHARKL KKYTSFSNFV 370 380 390 400 410 420 YGELMPDFLT IVFKKCGLNS NSIFMDLGSG VGNCVIQASL EFGCKLSFGC EIMDSASDMA 430 440 450 460 470 480 ELQLKELKSR CDLWGINLPP IDFSLRKSFV DNERVRELIP QCDVILINNF IFDAPLNKEV 490 500 510 520 530 540 EKVVQGLKAG SKIISLKSIR PPGYSINYDD MDNIFNRLHV ESFKLPENSV SWTYRSVGDY 550 560 570 YISTVLDAID ESIFCPPILG RIRKKESIKY TR

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382122 Genomic DNA. Translation: CAH01981.1.
RefSeq	XP_451588.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6CWV1.
Genome annotation databases
GeneID	2896923.
GenomeReviews	Gene locus KLLA0B01287g in contig CR382122_GR.
KEGG	kla:KLLA0B01287g.
Phylogenomic databases
HOGENOM	Q6CWV1.
OMA	LINNFIF.
Enzyme and pathway databases
BRENDA	2.1.1.43. 74088.
Family and domain databases
InterPro	IPR013110. DOT1. [Graphical view]
Pfam	PF08123. DOT1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DOT1_KLULA

Accession

Primary (citable) accession number: Q6CWV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	August 16, 2004
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)