Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q6CWV1

- DOT1_KLULA

UniProt

Q6CWV1 - DOT1_KLULA

Protein

Histone-lysine N-methyltransferase, H3 lysine-79 specific

Gene

DOT1

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (16 Aug 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones By similarity.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].PROSITE-ProRule annotation

    Enzyme regulationi

    Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei411 – 4111S-adenosyl-L-methioninePROSITE-ProRule annotation

    GO - Molecular functioni

    1. histone-lysine N-methyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator, Methyltransferase, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histone-lysine N-methyltransferase, H3 lysine-79 specific (EC:2.1.1.43)
    Alternative name(s):
    Histone H3-K79 methyltransferase
    Short name:
    H3-K79-HMTase
    Gene namesi
    Name:DOT1
    Ordered Locus Names:KLLA0B01287g
    OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
    Taxonomic identifieri284590 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
    ProteomesiUP000000598: Chromosome B

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 572572Histone-lysine N-methyltransferase, H3 lysine-79 specificPRO_0000270614Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi28985.Q6CWV1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6CWV1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini245 – 558314DOT1PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni361 – 3644S-adenosyl-L-methionine bindingPROSITE-ProRule annotation
    Regioni384 – 39310S-adenosyl-L-methionine bindingPROSITE-ProRule annotation
    Regioni448 – 4492S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.PROSITE-ProRule annotation
    Contains 1 DOT1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG294902.
    HOGENOMiHOG000112251.
    KOiK11427.
    OMAiNFVYGEL.
    OrthoDBiEOG7KH9VN.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR013110. DOT1.
    IPR025789. Histone_H3-K79_MeTrfase.
    IPR021162. Histone_H3-K79_MeTrfase_fungi.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF08123. DOT1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017570. Histone_H3-K79_MeTrfase. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51569. DOT1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6CWV1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTEATSSDA SVFSNMHSVD STRDNTRDNT RDNTPMNEGD TQGKRMTKST    50
    MALIEESMKY DMRSEYFLPM TFLRERRKPA RVESDEEDKL AEVKSKRRVS 100
    KKDQVKADIH STKIKKTGRP SNVKAGAKNT SKVTKQRSTK SQVSGKKRAR 150
    SISDGKVSSI DASRSLKASK KKHSSHADSL FLSNIEENVN PRPSFFNTTL 200
    IANPSSFTNK LTSSKMFVDD EEVTSLKGMK YILFPGVEEE YLICSKVQKF 250
    GYNPLPEIGQ SIESAVLLYF PNTYKTKGQE LVRSLNDAFE RSDDQSFEKI 300
    VLKYNDLIST IPRDLIVKNL SSNPDVPVSF VHFLLHTCYT RAIYPHARKL 350
    KKYTSFSNFV YGELMPDFLT IVFKKCGLNS NSIFMDLGSG VGNCVIQASL 400
    EFGCKLSFGC EIMDSASDMA ELQLKELKSR CDLWGINLPP IDFSLRKSFV 450
    DNERVRELIP QCDVILINNF IFDAPLNKEV EKVVQGLKAG SKIISLKSIR 500
    PPGYSINYDD MDNIFNRLHV ESFKLPENSV SWTYRSVGDY YISTVLDAID 550
    ESIFCPPILG RIRKKESIKY TR 572
    Length:572
    Mass (Da):64,796
    Last modified:August 16, 2004 - v1
    Checksum:iF830A57170781FF6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR382122 Genomic DNA. Translation: CAH01981.1.
    RefSeqiXP_451588.1. XM_451588.1.

    Genome annotation databases

    GeneIDi2896923.
    KEGGikla:KLLA0B01287g.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR382122 Genomic DNA. Translation: CAH01981.1 .
    RefSeqi XP_451588.1. XM_451588.1.

    3D structure databases

    ProteinModelPortali Q6CWV1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 28985.Q6CWV1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2896923.
    KEGGi kla:KLLA0B01287g.

    Phylogenomic databases

    eggNOGi NOG294902.
    HOGENOMi HOG000112251.
    KOi K11427.
    OMAi NFVYGEL.
    OrthoDBi EOG7KH9VN.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR013110. DOT1.
    IPR025789. Histone_H3-K79_MeTrfase.
    IPR021162. Histone_H3-K79_MeTrfase_fungi.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF08123. DOT1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017570. Histone_H3-K79_MeTrfase. 1 hit.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS51569. DOT1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Entry informationi

    Entry nameiDOT1_KLULA
    AccessioniPrimary (citable) accession number: Q6CWV1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3