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Q6CWQ8 (PUS1_KLULA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA pseudouridine synthase 1

EC=5.4.99.-
Alternative name(s):
tRNA pseudouridylate synthase 1
tRNA-uridine isomerase 1
Gene names
Name:PUS1
Ordered Locus Names:KLLA0B02244g
OrganismKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) [Complete proteome]
Taxonomic identifier284590 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces

Protein attributes

Sequence length553 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of transfer RNAs; at positions 34 and 36 of intron-containing precursor tRNA(Ile) and at position 35 in the intron-containing tRNA(Tyr) By similarity.

Catalytic activity

tRNA uridine = tRNA pseudouridine.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandMetal-binding
Zinc
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processpseudouridine synthesis

Inferred from electronic annotation. Source: InterPro

tRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

pseudouridine synthase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 553553tRNA pseudouridine synthase 1
PRO_0000057527

Sites

Active site1581Nucleophile By similarity
Binding site2141Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6CWQ8 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 569D70FDB3B1200C

FASTA55363,660
        10         20         30         40         50         60 
MSEEQNLRPV YDDDQPGEDT YKRGAIYKQT RSRKADYEDG ENSEKRQKPN DTDNGPTAVV 

        70         80         90        100        110        120 
TDLSNEKKET RSKDKDESVA LAVDADGNPI PQEVRLPKRK VAVMIGYCGT GYHGMQYNPP 

       130        140        150        160        170        180 
NDTIEKELFE AFVRAGAISK ANSTDLKKNG FQRAARTDKG VHAGGNVISL KLIIEDPEVK 

       190        200        210        220        230        240 
EKINNELPDQ IRVWDISRVN KAFDCRKMCS SRWYEYLLPT YSLIGPKPST YLYNEIEASK 

       250        260        270        280        290        300 
KEIPNVIQDD VESAQFWEAF STEAESKFTK EELEEIAAFV MPKDSFDENN ETYQKSKAFK 

       310        320        330        340        350        360 
KLEAEHKRAY RISKERLDRF RSALKQYEGT FNFHNFTLGK DFNDPSAKRF MKQITVSEPF 

       370        380        390        400        410        420 
VIGEAKTEWV SIKIHGQSFM LHQIRKMISM ATLITRCFSP LERIRQAYGQ EKINIPKAPA 

       430        440        450        460        470        480 
LGLLLEAPVY DGYNTRLQEF GYDPIDFSKY QSEMDTFKMK HIYDKIYLEE VNENVFNAFF 

       490        500        510        520        530        540 
SYIDTFNPSY NISQEQQQLK GQELQQPSQA VELVTEDLEQ KAPSDPTPSD EKGKKPQRPI 

       550 
FDFLTARGIQ LQD 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR382122 Genomic DNA. Translation: CAH02024.1.
RefSeqXP_451631.1. XM_451631.1.

3D structure databases

ProteinModelPortalQ6CWQ8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING28985.Q6CWQ8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2897526.
KEGGkla:KLLA0B02244g.

Phylogenomic databases

eggNOGCOG0101.
HOGENOMHOG000213984.
KOK06173.
OMAEWVSIKI.
OrthoDBEOG7DVDMQ.

Family and domain databases

Gene3D3.30.70.580. 1 hit.
3.30.70.660. 2 hits.
InterProIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERPTHR11142. PTHR11142. 1 hit.
PfamPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
SUPFAMSSF55120. SSF55120. 2 hits.
TIGRFAMsTIGR00071. hisT_truA. 1 hit.
ProtoNetSearch...

Entry information

Entry namePUS1_KLULA
AccessionPrimary (citable) accession number: Q6CWQ8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: August 16, 2004
Last modified: May 14, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families