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Q6CWQ8

- PUS1_KLULA

UniProt

Q6CWQ8 - PUS1_KLULA

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Protein

tRNA pseudouridine synthase 1

Gene

PUS1

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of transfer RNAs; at positions 34 and 36 of intron-containing precursor tRNA(Ile) and at position 35 in the intron-containing tRNA(Tyr).By similarity

Catalytic activityi

tRNA uridine = tRNA pseudouridine.

Cofactori

Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei158 – 1581NucleophileBy similarity
Binding sitei214 – 2141SubstrateBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. pseudouridine synthase activity Source: InterPro
  3. RNA binding Source: InterPro

GO - Biological processi

  1. pseudouridine synthesis Source: InterPro
  2. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase 1 (EC:5.4.99.-)
Alternative name(s):
tRNA pseudouridylate synthase 1
tRNA-uridine isomerase 1
Gene namesi
Name:PUS1
Ordered Locus Names:KLLA0B02244g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598: Chromosome B

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 553553tRNA pseudouridine synthase 1PRO_0000057527Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi28985.Q6CWQ8.

Structurei

3D structure databases

ProteinModelPortaliQ6CWQ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0101.
HOGENOMiHOG000213984.
InParanoidiQ6CWQ8.
KOiK06173.
OMAiEWVSIKI.
OrthoDBiEOG7DVDMQ.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 2 hits.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 2 hits.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6CWQ8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEEQNLRPV YDDDQPGEDT YKRGAIYKQT RSRKADYEDG ENSEKRQKPN
60 70 80 90 100
DTDNGPTAVV TDLSNEKKET RSKDKDESVA LAVDADGNPI PQEVRLPKRK
110 120 130 140 150
VAVMIGYCGT GYHGMQYNPP NDTIEKELFE AFVRAGAISK ANSTDLKKNG
160 170 180 190 200
FQRAARTDKG VHAGGNVISL KLIIEDPEVK EKINNELPDQ IRVWDISRVN
210 220 230 240 250
KAFDCRKMCS SRWYEYLLPT YSLIGPKPST YLYNEIEASK KEIPNVIQDD
260 270 280 290 300
VESAQFWEAF STEAESKFTK EELEEIAAFV MPKDSFDENN ETYQKSKAFK
310 320 330 340 350
KLEAEHKRAY RISKERLDRF RSALKQYEGT FNFHNFTLGK DFNDPSAKRF
360 370 380 390 400
MKQITVSEPF VIGEAKTEWV SIKIHGQSFM LHQIRKMISM ATLITRCFSP
410 420 430 440 450
LERIRQAYGQ EKINIPKAPA LGLLLEAPVY DGYNTRLQEF GYDPIDFSKY
460 470 480 490 500
QSEMDTFKMK HIYDKIYLEE VNENVFNAFF SYIDTFNPSY NISQEQQQLK
510 520 530 540 550
GQELQQPSQA VELVTEDLEQ KAPSDPTPSD EKGKKPQRPI FDFLTARGIQ

LQD
Length:553
Mass (Da):63,660
Last modified:August 16, 2004 - v1
Checksum:i569D70FDB3B1200C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR382122 Genomic DNA. Translation: CAH02024.1.
RefSeqiXP_451631.1. XM_451631.1.

Genome annotation databases

GeneIDi2897526.
KEGGikla:KLLA0B02244g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR382122 Genomic DNA. Translation: CAH02024.1 .
RefSeqi XP_451631.1. XM_451631.1.

3D structure databases

ProteinModelPortali Q6CWQ8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 28985.Q6CWQ8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2897526.
KEGGi kla:KLLA0B02244g.

Phylogenomic databases

eggNOGi COG0101.
HOGENOMi HOG000213984.
InParanoidi Q6CWQ8.
KOi K06173.
OMAi EWVSIKI.
OrthoDBi EOG7DVDMQ.

Family and domain databases

Gene3Di 3.30.70.580. 1 hit.
3.30.70.660. 2 hits.
InterProi IPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view ]
PANTHERi PTHR11142. PTHR11142. 1 hit.
Pfami PF01416. PseudoU_synth_1. 2 hits.
[Graphical view ]
SUPFAMi SSF55120. SSF55120. 2 hits.
TIGRFAMsi TIGR00071. hisT_truA. 1 hit.
ProtoNeti Search...

Entry informationi

Entry nameiPUS1_KLULA
AccessioniPrimary (citable) accession number: Q6CWQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: August 16, 2004
Last modified: October 29, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3