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Q6CWQ8

- PUS1_KLULA

UniProt

Q6CWQ8 - PUS1_KLULA

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Protein

tRNA pseudouridine synthase 1

Gene
PUS1, KLLA0B02244g
Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of transfer RNAs; at positions 34 and 36 of intron-containing precursor tRNA(Ile) and at position 35 in the intron-containing tRNA(Tyr) By similarity.

Catalytic activityi

tRNA uridine = tRNA pseudouridine.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei158 – 1581Nucleophile By similarity
Binding sitei214 – 2141Substrate By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. pseudouridine synthase activity Source: InterPro
  3. RNA binding Source: InterPro

GO - Biological processi

  1. pseudouridine synthesis Source: InterPro
  2. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase 1 (EC:5.4.99.-)
Alternative name(s):
tRNA pseudouridylate synthase 1
tRNA-uridine isomerase 1
Gene namesi
Name:PUS1
Ordered Locus Names:KLLA0B02244g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598: Chromosome B

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 553553tRNA pseudouridine synthase 1PRO_0000057527Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi28985.Q6CWQ8.

Structurei

3D structure databases

ProteinModelPortaliQ6CWQ8.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0101.
HOGENOMiHOG000213984.
KOiK06173.
OMAiEWVSIKI.
OrthoDBiEOG7DVDMQ.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 2 hits.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 2 hits.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6CWQ8-1 [UniParc]FASTAAdd to Basket

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MSEEQNLRPV YDDDQPGEDT YKRGAIYKQT RSRKADYEDG ENSEKRQKPN    50
DTDNGPTAVV TDLSNEKKET RSKDKDESVA LAVDADGNPI PQEVRLPKRK 100
VAVMIGYCGT GYHGMQYNPP NDTIEKELFE AFVRAGAISK ANSTDLKKNG 150
FQRAARTDKG VHAGGNVISL KLIIEDPEVK EKINNELPDQ IRVWDISRVN 200
KAFDCRKMCS SRWYEYLLPT YSLIGPKPST YLYNEIEASK KEIPNVIQDD 250
VESAQFWEAF STEAESKFTK EELEEIAAFV MPKDSFDENN ETYQKSKAFK 300
KLEAEHKRAY RISKERLDRF RSALKQYEGT FNFHNFTLGK DFNDPSAKRF 350
MKQITVSEPF VIGEAKTEWV SIKIHGQSFM LHQIRKMISM ATLITRCFSP 400
LERIRQAYGQ EKINIPKAPA LGLLLEAPVY DGYNTRLQEF GYDPIDFSKY 450
QSEMDTFKMK HIYDKIYLEE VNENVFNAFF SYIDTFNPSY NISQEQQQLK 500
GQELQQPSQA VELVTEDLEQ KAPSDPTPSD EKGKKPQRPI FDFLTARGIQ 550
LQD 553
Length:553
Mass (Da):63,660
Last modified:August 16, 2004 - v1
Checksum:i569D70FDB3B1200C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR382122 Genomic DNA. Translation: CAH02024.1.
RefSeqiXP_451631.1. XM_451631.1.

Genome annotation databases

GeneIDi2897526.
KEGGikla:KLLA0B02244g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR382122 Genomic DNA. Translation: CAH02024.1 .
RefSeqi XP_451631.1. XM_451631.1.

3D structure databases

ProteinModelPortali Q6CWQ8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 28985.Q6CWQ8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2897526.
KEGGi kla:KLLA0B02244g.

Phylogenomic databases

eggNOGi COG0101.
HOGENOMi HOG000213984.
KOi K06173.
OMAi EWVSIKI.
OrthoDBi EOG7DVDMQ.

Family and domain databases

Gene3Di 3.30.70.580. 1 hit.
3.30.70.660. 2 hits.
InterProi IPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view ]
PANTHERi PTHR11142. PTHR11142. 1 hit.
Pfami PF01416. PseudoU_synth_1. 2 hits.
[Graphical view ]
SUPFAMi SSF55120. SSF55120. 2 hits.
TIGRFAMsi TIGR00071. hisT_truA. 1 hit.
ProtoNeti Search...

Entry informationi

Entry nameiPUS1_KLULA
AccessioniPrimary (citable) accession number: Q6CWQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: August 16, 2004
Last modified: May 14, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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