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Q6CWQ8

- PUS1_KLULA

UniProt

Q6CWQ8 - PUS1_KLULA

Protein

tRNA pseudouridine synthase 1

Gene

PUS1

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of transfer RNAs; at positions 34 and 36 of intron-containing precursor tRNA(Ile) and at position 35 in the intron-containing tRNA(Tyr).By similarity

    Catalytic activityi

    tRNA uridine = tRNA pseudouridine.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei158 – 1581NucleophileBy similarity
    Binding sitei214 – 2141SubstrateBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. pseudouridine synthase activity Source: InterPro
    3. RNA binding Source: InterPro

    GO - Biological processi

    1. pseudouridine synthesis Source: InterPro
    2. tRNA processing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA pseudouridine synthase 1 (EC:5.4.99.-)
    Alternative name(s):
    tRNA pseudouridylate synthase 1
    tRNA-uridine isomerase 1
    Gene namesi
    Name:PUS1
    Ordered Locus Names:KLLA0B02244g
    OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
    Taxonomic identifieri284590 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
    ProteomesiUP000000598: Chromosome B

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 553553tRNA pseudouridine synthase 1PRO_0000057527Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi28985.Q6CWQ8.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6CWQ8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0101.
    HOGENOMiHOG000213984.
    KOiK06173.
    OMAiEWVSIKI.
    OrthoDBiEOG7DVDMQ.

    Family and domain databases

    Gene3Di3.30.70.580. 1 hit.
    3.30.70.660. 2 hits.
    InterProiIPR020103. PsdUridine_synth_cat_dom.
    IPR001406. PsdUridine_synth_TruA.
    IPR020097. PsdUridine_synth_TruA_a/b_dom.
    IPR020095. PsdUridine_synth_TruA_C.
    IPR020094. PsdUridine_synth_TruA_N.
    [Graphical view]
    PANTHERiPTHR11142. PTHR11142. 1 hit.
    PfamiPF01416. PseudoU_synth_1. 2 hits.
    [Graphical view]
    SUPFAMiSSF55120. SSF55120. 2 hits.
    TIGRFAMsiTIGR00071. hisT_truA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q6CWQ8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEEQNLRPV YDDDQPGEDT YKRGAIYKQT RSRKADYEDG ENSEKRQKPN    50
    DTDNGPTAVV TDLSNEKKET RSKDKDESVA LAVDADGNPI PQEVRLPKRK 100
    VAVMIGYCGT GYHGMQYNPP NDTIEKELFE AFVRAGAISK ANSTDLKKNG 150
    FQRAARTDKG VHAGGNVISL KLIIEDPEVK EKINNELPDQ IRVWDISRVN 200
    KAFDCRKMCS SRWYEYLLPT YSLIGPKPST YLYNEIEASK KEIPNVIQDD 250
    VESAQFWEAF STEAESKFTK EELEEIAAFV MPKDSFDENN ETYQKSKAFK 300
    KLEAEHKRAY RISKERLDRF RSALKQYEGT FNFHNFTLGK DFNDPSAKRF 350
    MKQITVSEPF VIGEAKTEWV SIKIHGQSFM LHQIRKMISM ATLITRCFSP 400
    LERIRQAYGQ EKINIPKAPA LGLLLEAPVY DGYNTRLQEF GYDPIDFSKY 450
    QSEMDTFKMK HIYDKIYLEE VNENVFNAFF SYIDTFNPSY NISQEQQQLK 500
    GQELQQPSQA VELVTEDLEQ KAPSDPTPSD EKGKKPQRPI FDFLTARGIQ 550
    LQD 553
    Length:553
    Mass (Da):63,660
    Last modified:August 16, 2004 - v1
    Checksum:i569D70FDB3B1200C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR382122 Genomic DNA. Translation: CAH02024.1.
    RefSeqiXP_451631.1. XM_451631.1.

    Genome annotation databases

    GeneIDi2897526.
    KEGGikla:KLLA0B02244g.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR382122 Genomic DNA. Translation: CAH02024.1 .
    RefSeqi XP_451631.1. XM_451631.1.

    3D structure databases

    ProteinModelPortali Q6CWQ8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 28985.Q6CWQ8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2897526.
    KEGGi kla:KLLA0B02244g.

    Phylogenomic databases

    eggNOGi COG0101.
    HOGENOMi HOG000213984.
    KOi K06173.
    OMAi EWVSIKI.
    OrthoDBi EOG7DVDMQ.

    Family and domain databases

    Gene3Di 3.30.70.580. 1 hit.
    3.30.70.660. 2 hits.
    InterProi IPR020103. PsdUridine_synth_cat_dom.
    IPR001406. PsdUridine_synth_TruA.
    IPR020097. PsdUridine_synth_TruA_a/b_dom.
    IPR020095. PsdUridine_synth_TruA_C.
    IPR020094. PsdUridine_synth_TruA_N.
    [Graphical view ]
    PANTHERi PTHR11142. PTHR11142. 1 hit.
    Pfami PF01416. PseudoU_synth_1. 2 hits.
    [Graphical view ]
    SUPFAMi SSF55120. SSF55120. 2 hits.
    TIGRFAMsi TIGR00071. hisT_truA. 1 hit.
    ProtoNeti Search...

    Entry informationi

    Entry nameiPUS1_KLULA
    AccessioniPrimary (citable) accession number: Q6CWQ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2006
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3