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Protein

Lysophospholipase NTE1

Gene

NTE1

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity).By similarity

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by organophosphorus esters.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei1170 – 11701By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi592 – 718127cNMP 1Add
BLAST
Nucleotide bindingi707 – 836130cNMP 2Add
BLAST

GO - Molecular functioni

  1. lysophospholipase activity Source: UniProtKB-EC

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
  2. phosphatidylcholine metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophospholipase NTE1 (EC:3.1.1.5)
Alternative name(s):
Intracellular phospholipase B
Neuropathy target esterase homolog
Gene namesi
Name:NTE1
Ordered Locus Names:KLLA0B05225g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598 Componenti: Chromosome B

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2222LumenalBy similarityAdd
BLAST
Transmembranei23 – 4321HelicalSequence AnalysisAdd
BLAST
Topological domaini44 – 14411398CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14411441Lysophospholipase NTE1PRO_0000295322Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi28985.Q6CWC2.

Structurei

3D structure databases

ProteinModelPortaliQ6CWC2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1137 – 1301165PatatinAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1168 – 11725GXSXG

Sequence similaritiesi

Belongs to the NTE family.Curated
Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation
Contains 1 patatin domain.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000048680.
InParanoidiQ6CWC2.
KOiK14676.
OMAiSPWTQTC.
OrthoDBiEOG70W3NS.

Family and domain databases

Gene3Di2.60.120.10. 4 hits.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR001423. LysoPLipase_patatin_CS.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF01734. Patatin. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 4 hits.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 2 hits.
PS01237. UPF0028. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6CWC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLTSYVLPR LKNILLLQFH ITLPLNYLVL LLLSTVIITY LFLRTRILSN
60 70 80 90 100
YSQLKDDVSD ENNINRKDYM DASSASFLLN REKHKGFTSY LDEFLSAIKI
110 120 130 140 150
FGYLEKPVFH ELTKSMKTEK LQEGEIVLLD DSVGFTIVVE GTLEILHKMD
160 170 180 190 200
NRHSNPSGDA AANATAFEPP SNDDGYLING EKFQLLNIVK TGNPLSSLVS
210 220 230 240 250
IMKLFSNRST HLNEGTHPST TNNTPGPGSP NLTGEINSFL DSTLPAKFGS
260 270 280 290 300
ADSANGNHPI SPMELESTFD NLSEASENDR SAKIPDIIAR AASDCTIAII
310 320 330 340 350
PSSSFQRLLV KYPRSASHII QMILTKLYRV TFKTAHTYLG LTNEIIFTEF
360 370 380 390 400
QSINKDNLKL PEYFRRSIIS YFTNRQDDTG SSASTIQKRP QLHRRDSNNS
410 420 430 440 450
LNYGSRHVVL NSRDQYNPGD LLSNVPLPRL NPQQRNDLSN TNSSSTLSRA
460 470 480 490 500
DYRPIILNNF SSSQFEETEV SSWRLALVEI IFQQLGITKD TIEPPISDDF
510 520 530 540 550
SLHDHSLDEK GLVRRSSYSS FTSLSSSIAT HSSNHLVTFL PRESQQFSRL
560 570 580 590 600
NRTGGKMASH SKRLNGSSRS NSRTDRSESF DHFRNENLGG DNQFSDFESV
610 620 630 640 650
KEDFSKCIKI LKFEEGETIL CQNSNPQGIY YLVSGEVDVI SETKDGNTDE
660 670 680 690 700
SYERTLYTAT EGYILGYLSS ILGCKSLVTL KVSKGPAYLG LIPYNDLERL
710 720 730 740 750
CDKYFMIYLK LSEILTNSLS PNLLRLDYFL EWIQLDSSET LFNQGDPANG
760 770 780 790 800
VYLVLNGRLR QLFYEDADSD IVTQMAELSK GESFGEVEVL TAIHRLNTVV
810 820 830 840 850
AIRDTELARI PRTLFEFLAV EHPSIMIHVS RMVAKKAMLM NFKSGIGFSG
860 870 880 890 900
SQPITKLIGD ETAMQKRYDF NLNIKSNKSS KKNELISNTV NYKTLTLLPI
910 920 930 940 950
TEGLPVEEFA YKLINALRQC GKTTIGLNQR TTLSHLGRHA FNKLSKLKQS
960 970 980 990 1000
GYFAELEELY EIVVYIADTP VSSSWTQTCI SQGDCILLLA DATCDPKIGE
1010 1020 1030 1040 1050
FERLLLKSKT TARTDLILLH PERYVVPGST SKWLKNRMWI QSHHHIQFTP
1060 1070 1080 1090 1100
MEKVAEPDPI PNIKPLSQLV EKFKENTKKT QENFVKFLPD SIKTTVETLS
1110 1120 1130 1140 1150
GKYIDPKPNP KFYTSVDPIK NDFLRLARTL SGQAVGLVLG GGGARGLSHL
1160 1170 1180 1190 1200
GIIKALEEQG IPIDIIGGTS IGSFVGGLYA MDYDLVPIYG RVKKFAGRVG
1210 1220 1230 1240 1250
SLWRMLSDLT WPVTSYTTGH EFNRGIWKSF RDYRIEDFWI SYYCNSTNIT
1260 1270 1280 1290 1300
ESVQEIHSSG FAWRYIRASM SLAGLLPPIV DNGNMLLDGG YVDNLPVTEM
1310 1320 1330 1340 1350
TQRGCKIVFA VDVGSVDDRT PMSYGDSLNG FWIVLNRWNP FSKHPNIPNM
1360 1370 1380 1390 1400
AEIQMRLGYV ASVNALERAK STPGVVYIRP PIENYATLDF GKFEEIYQVG
1410 1420 1430 1440
YAYGHDFLQH LQEKNELPPI AGTTTSAYAD KENMLQRRNS I
Length:1,441
Mass (Da):162,157
Last modified:August 16, 2004 - v1
Checksum:iB882F8E0F9533C00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382122 Genomic DNA. Translation: CAH02160.1.
RefSeqiXP_451767.1. XM_451767.1.

Genome annotation databases

GeneIDi2896879.
KEGGikla:KLLA0B05225g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382122 Genomic DNA. Translation: CAH02160.1.
RefSeqiXP_451767.1. XM_451767.1.

3D structure databases

ProteinModelPortaliQ6CWC2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi28985.Q6CWC2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2896879.
KEGGikla:KLLA0B05225g.

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000048680.
InParanoidiQ6CWC2.
KOiK14676.
OMAiSPWTQTC.
OrthoDBiEOG70W3NS.

Family and domain databases

Gene3Di2.60.120.10. 4 hits.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR001423. LysoPLipase_patatin_CS.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF01734. Patatin. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 4 hits.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 2 hits.
PS01237. UPF0028. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNTE1_KLULA
AccessioniPrimary (citable) accession number: Q6CWC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: August 16, 2004
Last modified: January 7, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.