Q6CVU2 (ALG1_KLULA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitobiosyldiphosphodolichol beta-mannosyltransferase
EC=2.4.1.142

Alternative name(s):
Asparagine-linked glycosylation protein 1
Beta-1,4-mannosyltransferase
GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase
GDP-mannose-dolichol diphosphochitobiose mannosyltransferase

Gene names

Name:	ALG1
Ordered Locus Names:	KLLA0B09405g

Organism

Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

284590 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man5 intermediate on the cytoplasmic surface of the ER By similarity.
Catalytic activity	GDP-mannose + chitobiosyldiphosphodolichol = GDP + beta-1,4-D-mannosylchitobiosyldiphosphodolichol.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase group 1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitobiosyldiphosphodolichol beta-mannosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Chitobiosyldiphosphodolichol beta-mannosyltransferase		PRO_0000080256
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 29	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	30 – 447	418	Lumenal Potential
Amino acid modifications
	Glycosylation	432	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6CVU2 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 6133706F353A8A4E	FASTA	447	51,620
	10 20 30 40 50 60 MSVFGFDNIP TWLWWLLAIY LATPFVLYVV QPYLFYEGKS TKTRILIVVL GDLGHSPRIL 70 80 90 100 110 120 YHARSFSKAG FQVELSGYVD SDIPTDILDD DNIEIHGLKK YGSEKGLLVK ALKQGLQLCS 130 140 150 160 170 180 MFWKLRAVDY ILLQNPPTIP ILPIAVVVKT FSRAKLIIDW HNLGYTILQM KFKDQFLHPL 190 200 210 220 230 240 VLLAYLIEWI FAKFANYHLT VTKAMKTYLV EKFGIDGKKI AVLYDRPGKQ FSPLKEADDR 250 260 270 280 290 300 EALLKQEFIA KYIPKEFDIS KDKIFVTSTS FTPDEDISVL IGSFKIYENS FQKFDQTLPR 310 320 330 340 350 360 ILCFITGKGP LQEKIVKQVQ DFKWDRVQVE FLWLSSEDYP KLLRLCDYGV SLHTSSSGLD 370 380 390 400 410 420 LPMKILDMFG SGLPVICMNY PVLDELVQQN VNGLKFADRR ELHEALIFSV KDEQVHQEIK 430 440 RGALRESKNR WNESWESALS ELKIIHK

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382122 Genomic DNA. Translation: CAH02340.1.
RefSeq	XP_451947.1. XM_451947.1.
3D structure databases
ProteinModelPortal	Q6CVU2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6CVU2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2896930.
GenomeReviews	Gene locus KLLA0B09405g in contig CR382122_GR.
KEGG	kla:KLLA0B09405g.
Phylogenomic databases
eggNOG	fuNOG05192.
HOGENOM	HBG319770.
OMA	LQNPPGL.
OrthoDB	EOG4WWVT5.
Family and domain databases
InterPro	IPR001296. Glyco_trans_1. [Graphical view]
KO	K03842.
Pfam	PF00534. Glycos_transf_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG1_KLULA

Accession

Primary (citable) accession number: Q6CVU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	August 16, 2004
Last modified:	December 14, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program