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Q6CV81 (DOHH_KLULA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxyhypusine hydroxylase

Short name=DOHH
EC=1.14.99.29
Alternative name(s):
Deoxyhypusine dioxygenase
Deoxyhypusine monooxygenase
Gene names
Name:LIA1
Ordered Locus Names:KLLA0B14080g
OrganismKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) [Complete proteome]
Taxonomic identifier284590 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces

Protein attributes

Sequence length322 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor By similarity. HAMAP-Rule MF_03101

Catalytic activity

[eIF5A]-deoxyhypusine + AH2 + O2 = [eIF5A]-hypusine + A + H2O. HAMAP-Rule MF_03101

Cofactor

Binds 2 Fe2+ ions per subunit By similarity. HAMAP-Rule MF_03101

Pathway

Protein modification; eIF5A hypusination. HAMAP-Rule MF_03101

Subcellular location

Cytoplasm. Nucleus By similarity HAMAP-Rule MF_03101.

Sequence similarities

Belongs to the deoxyhypusine hydroxylase family.

Contains 3 HEAT-like PBS-type repeats.

Ontologies

Keywords
   Biological processHypusine biosynthesis
   Cellular componentCytoplasm
Nucleus
   DomainRepeat
   LigandIron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processpeptidyl-lysine modification to peptidyl-hypusine

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondeoxyhypusine monooxygenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 322322Deoxyhypusine hydroxylase HAMAP-Rule MF_03101
PRO_0000283667

Regions

Repeat76 – 10227HEAT-like PBS-type 1 HAMAP-Rule MF_03101
Repeat109 – 13527HEAT-like PBS-type 2 HAMAP-Rule MF_03101
Repeat267 – 29327HEAT-like PBS-type 3 HAMAP-Rule MF_03101

Sites

Metal binding781Iron 1 By similarity
Metal binding791Iron 1 By similarity
Metal binding1111Iron 1 By similarity
Metal binding1121Iron 1 By similarity
Metal binding2361Iron 2 By similarity
Metal binding2371Iron 2 By similarity
Metal binding2691Iron 2 By similarity
Metal binding2701Iron 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6CV81 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: F0D713105D85C20D

FASTA32236,359
        10         20         30         40         50         60 
MSTNFEKHFE VDVDNCSLEQ LRDILVNNSG KAPLANRFRA LFNLKGHAEE FASKPEDALK 

        70         80         90        100        110        120 
ATQYLAEAFG DESELLKHEV AYVLGQTKNM AGAPLLRDVL ADDKQQCMVR HEAAEALGAL 

       130        140        150        160        170        180 
NDVDSLDILE KYFKEDPLLE IRQTCELAID RIKWETSEEG RREALQESLY SSIDPAPPFS 

       190        200        210        220        230        240 
LEKDYKIQEL KDILNDQNRP LFERYRAMFR LRDIGNDEAC LALASGFDDP SALFKHEIAY 

       250        260        270        280        290        300 
VFGQICNPVV VPHLKEVLAR PEEAPMVRHE AAEALGSIAT DDVLPVLKEH LKDSDSVVRE 

       310        320 
SAIVALDMYE YENSNDLEYA PV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR382122 Genomic DNA. Translation: CAH02551.1.
RefSeqXP_452158.1. XM_452158.1.

3D structure databases

ProteinModelPortalQ6CV81.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING28985.Q6CV81.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2897036.
KEGGkla:KLLA0B14080g.

Phylogenomic databases

eggNOGCOG1413.
HOGENOMHOG000248665.
KOK06072.
OMARESCQVA.
OrthoDBEOG7QC85Z.

Enzyme and pathway databases

UniPathwayUPA00354.

Family and domain databases

Gene3D1.25.10.10. 1 hit.
HAMAPMF_03101. Deoxyhypusine_hydroxylase.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027517. Deoxyhypusine_hydroxylase.
IPR021133. HEAT_type_2.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
SMARTSM00567. EZ_HEAT. 5 hits.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
PROSITEPS50077. HEAT_REPEAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDOHH_KLULA
AccessionPrimary (citable) accession number: Q6CV81
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: August 16, 2004
Last modified: July 9, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways