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Protein

Deoxyhypusine hydroxylase

Gene

LIA1

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.UniRule annotation

Catalytic activityi

[eIF5A]-deoxyhypusine + AH2 + O2 = [eIF5A]-hypusine + A + H2O.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 2 Fe2+ ions per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi78 – 781Iron 1UniRule annotation
Metal bindingi79 – 791Iron 1UniRule annotation
Metal bindingi111 – 1111Iron 1UniRule annotation
Metal bindingi112 – 1121Iron 1UniRule annotation
Metal bindingi236 – 2361Iron 2UniRule annotation
Metal bindingi237 – 2371Iron 2UniRule annotation
Metal bindingi269 – 2691Iron 2UniRule annotation
Metal bindingi270 – 2701Iron 2UniRule annotation

GO - Molecular functioni

  1. deoxyhypusine monooxygenase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. peptidyl-lysine modification to peptidyl-hypusine Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Hypusine biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine hydroxylaseUniRule annotation (EC:1.14.99.29UniRule annotation)
Short name:
DOHHUniRule annotation
Alternative name(s):
Deoxyhypusine dioxygenaseUniRule annotation
Deoxyhypusine monooxygenaseUniRule annotation
Gene namesi
Name:LIA1UniRule annotation
Ordered Locus Names:KLLA0B14080g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598 Componenti: Chromosome B

Subcellular locationi

  1. Cytoplasm UniRule annotation
  2. Nucleus UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322Deoxyhypusine hydroxylasePRO_0000283667Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi28985.Q6CV81.

Structurei

3D structure databases

ProteinModelPortaliQ6CV81.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati76 – 10227HEAT-like PBS-type 1Add
BLAST
Repeati109 – 13527HEAT-like PBS-type 2Add
BLAST
Repeati267 – 29327HEAT-like PBS-type 3Add
BLAST

Sequence similaritiesi

Belongs to the deoxyhypusine hydroxylase family.UniRule annotation
Contains 3 HEAT-like PBS-type repeats.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1413.
HOGENOMiHOG000248665.
InParanoidiQ6CV81.
KOiK06072.
OMAiHEAAVPQ.
OrthoDBiEOG7QC85Z.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
HAMAPiMF_03101. Deoxyhypusine_hydroxylase.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027517. Deoxyhypusine_hydroxylase.
IPR021133. HEAT_type_2.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
SMARTiSM00567. EZ_HEAT. 5 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6CV81-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTNFEKHFE VDVDNCSLEQ LRDILVNNSG KAPLANRFRA LFNLKGHAEE
60 70 80 90 100
FASKPEDALK ATQYLAEAFG DESELLKHEV AYVLGQTKNM AGAPLLRDVL
110 120 130 140 150
ADDKQQCMVR HEAAEALGAL NDVDSLDILE KYFKEDPLLE IRQTCELAID
160 170 180 190 200
RIKWETSEEG RREALQESLY SSIDPAPPFS LEKDYKIQEL KDILNDQNRP
210 220 230 240 250
LFERYRAMFR LRDIGNDEAC LALASGFDDP SALFKHEIAY VFGQICNPVV
260 270 280 290 300
VPHLKEVLAR PEEAPMVRHE AAEALGSIAT DDVLPVLKEH LKDSDSVVRE
310 320
SAIVALDMYE YENSNDLEYA PV
Length:322
Mass (Da):36,359
Last modified:August 16, 2004 - v1
Checksum:iF0D713105D85C20D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382122 Genomic DNA. Translation: CAH02551.1.
RefSeqiXP_452158.1. XM_452158.1.

Genome annotation databases

GeneIDi2897036.
KEGGikla:KLLA0B14080g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382122 Genomic DNA. Translation: CAH02551.1.
RefSeqiXP_452158.1. XM_452158.1.

3D structure databases

ProteinModelPortaliQ6CV81.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi28985.Q6CV81.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2897036.
KEGGikla:KLLA0B14080g.

Phylogenomic databases

eggNOGiCOG1413.
HOGENOMiHOG000248665.
InParanoidiQ6CV81.
KOiK06072.
OMAiHEAAVPQ.
OrthoDBiEOG7QC85Z.

Enzyme and pathway databases

UniPathwayiUPA00354.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
HAMAPiMF_03101. Deoxyhypusine_hydroxylase.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027517. Deoxyhypusine_hydroxylase.
IPR021133. HEAT_type_2.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
SMARTiSM00567. EZ_HEAT. 5 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDOHH_KLULA
AccessioniPrimary (citable) accession number: Q6CV81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: August 16, 2004
Last modified: January 7, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.