Reviewed, UniProtKB/Swiss-Prot Q6CUV2 (ATG26_KLULA)

Names and origin

Protein names

Recommended name:
    Sterol 3-beta-glucosyltransferase
    EC=2.4.1.173
Alternative name(s):
    Autophagy-related protein 26

Gene names

Name:	ATG26
Ordered Locus Names:	KLLA0C02035g

Organism

Kluyveromyces lactis (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

28985 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	1209 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in decane metabolism and autophagy. Involved in the biosynthesis of sterol glucoside By similarity.
Catalytic activity	UDP-glucose + a sterol = UDP + a sterol 3-beta-D-glucoside.
Subcellular location	Membrane; Peripheral membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 28 family. Contains 2 GRAM domains. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Autophagy Lipid synthesis Protein transport Steroid biosynthesis Sterol biosynthesis Transport
Cellular component	Membrane
Domain	Repeat
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro lipid glycosylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sterol 3-beta-glucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1209	1209	Sterol 3-beta-glucosyltransferase		PRO_0000215611
Regions
	Domain	167 – 217	51	GRAM 1
	Domain	218 – 315	98	PH
	Domain	568 – 634	67	GRAM 2

Sequences

	Sequence		Length	Mass (Da)
	Q6CUV2-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 0D5653C52FE15CF7	FASTA	1,209	136,047
	10 20 30 40 50 60 MPKDSSEHQK SPSRFTRSMF VDPRAFVRRS VSPVDQLCHS VADLRFVSSN TETLESRLQA 70 80 90 100 110 120 LSKKFDNGSE NHGQDTDTVE DVAKTQYMAK SFAGLIATAS MYVGINELGD KEENDEDELE 130 140 150 160 170 180 LMNDAASVLD TSSQANQTLF EVSIEMNADA ISQISTDSKS RKELIRERLI KKFLPNDDEK 190 200 210 220 230 240 YIEEYPCWLL RDIMIQGHAY LTNKHLFFFA FIPNFESDFN VTGSLRLISG HVLSKSHRYW 250 260 270 280 290 300 VVLKGHTLSF HNSSTDLYFP LLTIDLRDIS SVQMTSSENN PTKFELSIKD QSLVLKADSF 310 320 330 340 350 360 HSARHWVSSI KKQMFASQHS DTNTMTIKIP LQNIVDLEET SILDKSGTLR IKALENLSTY 370 380 390 400 410 420 AVDEYFFVFF KGNANAMKQK VNSLLKDLEM NGSQILVNFN KVDSPLGINE KLPDLNYDDP 430 440 450 460 470 480 SNENNLVDDA DINSAETDTL SPGTMQSALT LQQTSSAFSP RHSAYPRKVK KKLKSMAGSL 490 500 510 520 530 540 KLGSPSKFTK LEDDIIIEHY SPGLINDQTI DYDKDSKSII SRLTPKKFQN VPLMWAADPV 550 560 570 580 590 600 HFNSDDGIVF PLDDKYTADA DISNQSNVRF RQHFSFDETA NLVASYHGYL NRNVPIYGKI 610 620 630 640 650 660 YISDKNICFR SLLPGVSTKT VLPLEDVENC YKETRFRFGY FGLVIVIHGH EELFLEFGNK 670 680 690 700 710 720 NARDDCEFVM IKVMDAVSSH RSTLKRKSTA EVVHRLSEAA SLKLLEEKIS EQGFDIPLIV 730 740 750 760 770 780 EKNPYFTTVI KPSKSYKFGL LTIGSRGDVQ PYIALAKGLQ AEGHEVIILT HGEFKDWIVS 790 800 810 820 830 840 HNIGFREISG NPAELISLMV QHGSMNMGLL RDASTNFSTW ISSLLDTAWE GCQGIDILIE 850 860 870 880 890 900 SPSAMAGIHI AEALRIPYFR AFTMPWTRTR AYPHAFIVPD QKRGGNYNYF THVLFENIFW 910 920 930 940 950 960 KGISGKVNEW RETKLKLPKT NLVSMQQNRV PFLYNVSPIV FPPSVDFNEW IKVTGYWFLD 970 980 990 1000 1010 1020 EKRSYKPPAE FMEFLNKARE LKKKVVYIGF GSIVVNDPEK MTDTIIEAVR DAGVYCVLNK 1030 1040 1050 1060 1070 1080 GWSNRFGDPL AKKIDKELPS YIYNSGDVPH DWLFTKIDAT VHHGGSGTTG ASLRAGLPTI 1090 1100 1110 1120 1130 1140 IKPFFGDQFF YASRVEDIGA GVALKKLNRS SLAKALKEVT TNTRIIQKAR QIGESISKEH 1150 1160 1170 1180 1190 1200 GVATAIGAIY SELGYARSLI KTKNPVDDKE MEAASTKLSN DAVVTAKGNE KEEYSSEGSG SNDGSWLLI

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382123 Genomic DNA. Translation: CAH01138.1.
RefSeq	XP_452287.1.
3D structure databases
SMR	Q6CUV2. Positions 231-312, 737-1153.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6CUV2.
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Genome annotation databases
GeneID	2891856.
GenomeReviews	Gene locus KLLA0C02035g in contig CR382123_GR.
KEGG	kla:KLLA0C02035g.
Phylogenomic databases
HOGENOM	HBG332791.
OMA	EYPCWLL.
OrthoDB	EOG9908S8.
PhylomeDB	Q6CUV2.
Enzyme and pathway databases
BRENDA	2.4.1.173. 74088.
Family and domain databases
InterPro	IPR004276. Glyco_trans_28. IPR004182. GRAM. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002213. UDP_glucos_trans. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
PANTHER	PTHR11926. UDP_glucos_trans. 1 hit.
Pfam	PF03033. Glyco_transf_28. 1 hit. PF02893. GRAM. 2 hits. PF00169. PH. 1 hit. PF00201. UDPGT. 1 hit. [Graphical view]
SMART	SM00568. GRAM. 2 hits. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG26_KLULA

Accession

Primary (citable) accession number: Q6CUV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	August 16, 2004
Last modified:	January 19, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)