Q6CUM6 (TRM10_KLULA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine(9)-N1)-methyltransferase
EC=2.1.1.221

Alternative name(s):
tRNA methyltransferase 10
tRNA(m1G9)-methyltransferase
Short name=tRNA(m1G9)MTase

Gene names

Name:	TRM10
Ordered Locus Names:	KLLA0C03740g

Organism

Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

284590 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in cytoplasmic tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine9 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine9 in tRNA.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family. TRM10 subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	tRNA (guanine(9)-N1)-methyltransferase		PRO_0000060517

Sequences

	Sequence		Length	Mass (Da)
	Q6CUM6 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: FFD7198D93416BF6	FASTA	298	34,387
	10 20 30 40 50 60 MSDTSENSNA EIPADTSDVK DKPKPIVRAP QFPPPPEGIS KSQWKKICRK KRFEETRAEY 70 80 90 100 110 120 AQIRKEKRNR AKLARREKLK EYTDRGEEIP EELKRPPKVN LNQSDSGISI ILDCSFDDLM 130 140 150 160 170 180 NDREIVSLST QVTRAYSSNK RENNYAKIKV TSFDKRLKQR FDNDLSNSNY TKWKNFEFTA 190 200 210 220 230 240 DPTLPTENAV YLTADTEEKL DTLEPGTTYI VGGIVDKNRH KNLCYNKAKE LNIPTKRLPI 250 260 270 280 290 GEFINLAGRK VLTTSHMVQL MLRYFDNKDW KEAFESVLPP RKLEVDSTKE DSETASAE

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382123 Genomic DNA. Translation: CAH01214.1.
RefSeq	XP_452363.1. XM_452363.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6CUM6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2892404.
GenomeReviews	Gene locus KLLA0C03740g in contig CR382123_GR.
KEGG	kla:KLLA0C03740g.
Phylogenomic databases
eggNOG	fuNOG04600.
HOGENOM	HBG717479.
OMA	SNVERKQ.
OrthoDB	EOG48D43Z.
Family and domain databases
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR007356. tRNA_m1G_MeTrfase_euk. IPR016653. tRNA_m1G_mtfrase_met. [Graphical view]
KO	K15445.
PANTHER	PTHR13563. DUF425. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF016323. tRNA_m1G_mtfrase_met. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRM10_KLULA

Accession

Primary (citable) accession number: Q6CUM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	August 16, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program