ID   UBC2_KLULA              Reviewed;         164 AA.
AC   Q6CUD9;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   16-JUN-2009, entry version 43.
DE   RecName: Full=Ubiquitin-conjugating enzyme E2 2;
DE            EC=6.3.2.19;
DE   AltName: Full=Ubiquitin-protein ligase UBC2;
DE   AltName: Full=Ubiquitin carrier protein UBC2;
GN   Name=UBC2; OrderedLocusNames=KLLA0C05632g;
OS   Kluyveromyces lactis (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=28985;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S.,
RA   Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E.,
RA   Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V.,
RA   Barnay S., Blanchin S., Beckerich J.-M., Beyne E., Bleykasten C.,
RA   Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A.,
RA   Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A.,
RA   Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R.,
RA   Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H.,
RA   Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O.,
RA   Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A.,
RA   Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B.,
RA   Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A.,
RA   Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J.,
RA   Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC       proteins. Plays a role in transcription regulation by catalyzing
CC       the monoubiquitination of histone H2B to form H2BK123ub1.
CC       H2BK123ub1 gives a specific tag for epigenetic transcriptional
CC       activation and is also a prerequisite for H3K4me and H3K79me
CC       formation. Also involved in postreplication repair of UV-damaged
CC       DNA, in N-end rule-dependent protein degradation and in
CC       sporulation (By similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP +
CC       diphosphate + protein N-ubiquityllysine.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By
CC       similarity).
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
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DR   EMBL; CR382123; CAH01301.1; -; Genomic_DNA.
DR   RefSeq; XP_452450.1; -.
DR   SMR; Q6CUD9; 2-154.
DR   GeneID; 2892656; -.
DR   KEGG; kla:KLLA0C05632g; -.
DR   HOGENOM; Q6CUD9; -.
DR   OMA; Q6CUD9; YANGEIC.
DR   BRENDA; 6.3.2.19; 74088.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004842; F:ubiquitin-protein ligase activity; IEA:EC.
DR   GO; GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0019941; P:modification-dependent protein catabolic pr...; IEA:UniProtKB-KW.
DR   GO; GO:0043687; P:post-translational protein modification; IEA:InterPro.
DR   GO; GO:0051246; P:regulation of protein metabolic process; IEA:InterPro.
DR   GO; GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cel...; IEA:UniProtKB-KW.
DR   GO; GO:0006350; P:transcription; IEA:UniProtKB-KW.
DR   InterPro; IPR016135; UBQ-conjugat/RWD-like.
DR   InterPro; IPR000608; UBQ-conjugat_E2.
DR   Gene3D; G3DSA:3.10.110.10; UBQ-conjugat_E2; 1.
DR   Pfam; PF00179; UQ_con; 1.
DR   ProDom; PD000461; UBQ_conjugat; 1.
DR   SMART; SM00212; UBCc; 1.
DR   PROSITE; PS00183; UBIQUITIN_CONJUGAT_1; 1.
DR   PROSITE; PS50127; UBIQUITIN_CONJUGAT_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chromatin regulator; Complete proteome; Cytoplasm;
KW   DNA damage; DNA repair; Ligase; Nucleotide-binding; Nucleus;
KW   Sporulation; Transcription; Transcription regulation;
KW   Ubl conjugation pathway.
FT   CHAIN         1    164       Ubiquitin-conjugating enzyme E2 2.
FT                                /FTId=PRO_0000082533.
FT   ACT_SITE     88     88       Glycyl thioester intermediate (By
FT                                similarity).
SQ   SEQUENCE   164 AA;  18871 MW;  CE7C16806938D62A CRC64;
     MSTPARRRLM RDFKRMKEDS PPGVSASPLP DNVMIWNAMI IGPADTPYED GTFRLLLEFD
     EEYPNKPPHV KFLSEMFHPN VYANGEICLD ILQNRWTPTY DVASILTSIQ SLFNDPNPAS
     PANVEAATLF QDHKSQYVKR VKETVEKSWE DDMEDMADED EDEE
//