Reviewed, UniProtKB/Swiss-Prot Q6CTX8 (PYRD2_KLULA)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase, mitochondrial
      Short name=DHOdehase
      Short name=DHODase
      Short name=DHOD
    EC=1.3.5.2
Alternative name(s):
    Dihydroorotate oxidase

Gene names

Name:	URA9
Ordered Locus Names:	KLLA0C09240g

Organism

Kluyveromyces lactis (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

28985 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + a quinone = orotate + a quinol.
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
Subcellular location	Mitochondrion inner membrane By similarity; Single-pass membrane protein Potential.
Miscellaneous	K.lactis has two isoforms of DHODase, a cytoplasmic isoform and a mitochondrial isoform.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 16	16	Mitochondrion Potential
	Chain	17 – 445	429	Dihydroorotate dehydrogenase, mitochondrial		PRO_0000029893
Regions
	Transmembrane	39 – 56	18	Potential
Sites
	Active site	254	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6CTX8-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 74F746F6773A3492	FASTA	445	48,071
	10 20 30 40 50 60 MNSGFPRILS KKLFTLNQSQ FFVKNGMVPL KAGISGPKLL KYTVGIAIGS FAGFYFSNSR 70 80 90 100 110 120 SAFHEYVLCP MLRLVTPDAE DGHKLGIWFL KNGLAPRLWF DNDDKVLNVN IFGKKLTNPI 130 140 150 160 170 180 GCAAGLDKNG DAIDGILSGG FGYIEIGSVT PLPQPGNPRP RFFRLPLDDA VINRYGFNSS 190 200 210 220 230 240 GHDTVVNTLQ SRITSFINSY MFKDNSVENL SLYKDKLLGV NLGKNKTGDE VQDYLKGVES 250 260 270 280 290 300 FQKYADVLVI NVSSPNTPGL RSLQKESILT DLLTQVVAKR DSLVTSGNAL GAKTHKPPVL 310 320 330 340 350 360 VKVAPDLVEE EIKSIAEAAK KSKVDGIIIS NTTIQRPTTL ITEDSDLVSQ AGGLSGKPLK 370 380 390 400 410 420 PLALKALKTM AKYTKGSGLV LVGCGGISSG ADAIEFAKAG ASMVELYTAY AYKGPGLIAK 430 440 IKDETTELLK KENKTWSEII GEDIK

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382123 Genomic DNA. Translation: CAH01462.1.
RefSeq	XP_452611.1.
3D structure databases
SMR	Q6CTX8. Positions 62-445.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6CTX8.
Genome annotation databases
GeneID	2891871.
GenomeReviews	Gene locus KLLA0C09240g in contig CR382123_GR.
KEGG	kla:KLLA0C09240g.
Phylogenomic databases
eggNOG	fuNOG05791.
HOGENOM	HBG351027.
OMA	AALNRMG.
OrthoDB	EOG9CNS7M.
PhylomeDB	Q6CTX8.
Enzyme and pathway databases
BRENDA	1.3.3.1. 74088.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD2_KLULA

Accession

Primary (citable) accession number: Q6CTX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	August 16, 2004
Last modified:	February 9, 2010
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)