Reviewed, UniProtKB/Swiss-Prot Q6CT22 (CET1_KLULA)

Names and origin

Protein names

Recommended name:
    mRNA-capping enzyme subunit beta
    EC=3.1.3.33
Alternative name(s):
    Polynucleotide 5'-triphosphatase
    mRNA 5'-triphosphatase
      Short name=TPase

Gene names

Name:	CET1
Ordered Locus Names:	KLLA0C16049g

Organism

Kluyveromyces lactis (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

28985 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end By similarity.
Catalytic activity	A 5'-phosphopolynucleotide + H(2)O = a polynucleotide + phosphate.
Cofactor	Divalent ions By similarity.
Subunit structure	The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an RNA 5'-triphosphatase By similarity.
Subcellular location	NucleusBy similarity.
Sequence similarities	Belongs to the fungal TPase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	mRNA-capping enzyme subunit beta		PRO_0000210116

Sequences

	Sequence		Length	Mass (Da)
	Q6CT22-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 64BD4598D3E67D45	FASTA	556	62,743
	10 20 30 40 50 60 MKPSRGLSLT DLVNHDDAPP LNNGDNNVKQ EEVQAENLAT NPASVLAPGP VIVDTLPPVE 70 80 90 100 110 120 APVSTSDTGN TSHTGAAPQT AVTAESDETD TDDEPGEIVF ENTKFRFDDE EQQPQKDKLV 130 140 150 160 170 180 KGTSSEKKDK QVNAATKEIQ LDSKPVKEQS PKTKDEGDIP VEDADKADNK VETQKKENGI 190 200 210 220 230 240 KQEVEPVEQD GKKSVEPAKQ SKEDSKKEKD IFQQKTSNAS VKNNIKKDLK ILSELSSSSL 250 260 270 280 290 300 PKRYNVPPIW ARKWKPTVKA LQAIDSSNLK LDDSILGFIP EDDLTKSVQD WIYATLIAVE 310 320 330 340 350 360 PELRQFIEVE MKYGLIIDPS TSNRVNPPVS SQCVFTDLDS TMKPDVDERV FDEFNRYIKN 370 380 390 400 410 420 LSELNENMGK FNIIDSHASD LSYRVRTHTE RPKFLRMTRD VNTGRIAQFI EKRKISQILL 430 440 450 460 470 480 YSPKDSYDTK ISISLELPVP ENDPPEKYKN HTPTGHRLKK RTSYIHNDSC TRFDITRVEN 490 500 510 520 530 540 KPIRVNNKNE KEPESDTTYE VELEINTPAL LNAFDNIQHD SKEYAAIVRT FLNNGTIVRR 550 KLSSLSYDIY KGSNKL

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382123 Genomic DNA. Translation: CAH01768.1.
RefSeq	XP_452917.1.
3D structure databases
SMR	Q6CT22. Positions 244-547.
ModBase	Search...
Genome annotation databases
GeneID	2892078.
KEGG	kla:KLLA0C16049g.
Phylogenomic databases
HOGENOM	Q6CT22.
Family and domain databases
InterPro	IPR004206. mRNA_triPase. [Graphical view]
Pfam	PF02940. mRNA_triPase. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

CET1_KLULA

Accession

Primary (citable) accession number: Q6CT22

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	August 16, 2004
Last modified:	July 22, 2008
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)