Q6CSR1 (MSH3_KLULA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA mismatch repair protein MSH3

Alternative name(s):
MutS protein homolog 3

Gene names

Name:	MSH3
Ordered Locus Names:	KLLA0C18590g

Organism

Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

284590 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces › Kluyveromyces lactis

Protein attributes

Sequence length	1029 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta, which binds to DNA mismatches thereby initiating DNA repair. MSH3 provides substrate-binding and substrate-specificity to the complex. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. Acts mainly to repair insertion-deletion loops (IDLs) from 2 to 13 nucleotides in size, but can also repair base-base and single insertion-deletion mismatches that occur during replication. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions By similarity.
Subunit structure	Heterodimer consisting of MSH2-MSH3 (MutS beta). Forms a ternary complex with MutL alpha (MLH1-PMS1) By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the DNA mismatch repair MutS family. MSH3 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mismatch repair Inferred from electronic annotation. Source: InterPro
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW mismatched DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1029	1029	DNA mismatch repair protein MSH3		PRO_0000338522
Regions
	Nucleotide binding	802 – 809	8	ATP Potential
	Region	143 – 270	128	Mispair-binding domain By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6CSR1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 240FFB8C8B61359E	FASTA	1,029	118,522
	10 20 30 40 50 60 MYQPTISHFF KSSQPSSQTD TRQDEDQRLD IESHEQRGGA NMNKDELINI LGSDSESDQD 70 80 90 100 110 120 LDEDLDQDQN NNQDQDQEQD RDLASKANNG RSTLSAERIE RRPDFNEKLK TIMRKRNAGS 130 140 150 160 170 180 MINTGSDDEN DDNDDVKSTK RTKANNKKLT ELDQQFKELK LKHMDTILCV RVGYKYKFFA 190 200 210 220 230 240 KDAEIVSNIL QIKLVPGKKT LDESDPNDRN YRKFQYCSIP DTRLHVHLQR LVFFNYKVAV 250 260 270 280 290 300 VEQTETSALK KNNNSGSLFT REIKNIFTKV SYGINETFDK SEDRILGDLT SVWAISINET 310 320 330 340 350 360 SKMRKVNLIS VQLNSGEIVH DQFSDDILLN VNLEARIRYL NPTEIITEEE LPPSIRTIFT 370 380 390 400 410 420 KLNQDIQFYQ SHKEACPNLF DALQGLDLNN ELKRLLSVLH SYLSTFENTK VLYFASNYSS 430 440 450 460 470 480 FTAKNFMVLP RNTIESLEIF ENSTTNKTNG SLLWVMDHTR TQFGYRLLRK WISKPLIDLK 490 500 510 520 530 540 SILDRQDAIT CIMKEVHSIF FESFNELLRK SIDLERALNR IAYGSTSRKE VYFFLKQIAT 550 560 570 580 590 600 FASLFKSHHT FIHDQLHKEN SALRKTSCLL FNILQNLDAF FSATDLPLFL QMINVDAALD 610 620 630 640 650 660 KDSHKNVIEF FNLNKYDFPE GLLHKYRDIE EVKTELDDEL QNIKRVLKRP TLSYKDTKDY 670 680 690 700 710 720 LIEVRNTQAK TVPSNWVKVN STKAVSRFRT PKTEELVGKL LYHNDLLNLL AEDEFKRFLQ 730 740 750 760 770 780 RIVDRYAEIK TCINNLATYD CILSLAATSS NVNYVKPKLT ELHQKVKVKN GRNPIIESLD 790 800 810 820 830 840 VNYVPNDVLM SSNSGKINII TGPNMGGKSS YIRQVALLVI MTQIGCYIPA DSAEMSICDR 850 860 870 880 890 900 IFTRIGSHDD LLNAKSTFQV EMSEVLHILN SSTPRSLLLL DEVGRGTGTH DGLSISFAIL 910 920 930 940 950 960 NYFVYLADNC PLVLFITHYS ALCQIDSKLI ANYHMSYIEK HQPGEKWTNV IFLYKLVLGQ 970 980 990 1000 1010 1020 AHNSYGFNVA KLSNIPTEII NRAFEVSEEK ILSSKHHNFL EIMKALKRVN ERKLNKEALK KIQAFIEDI

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382123 Genomic DNA. Translation: CAH01879.1.
RefSeq	XP_453028.1. XM_453028.1.
3D structure databases
HSSP	HSSP built from PDB template 1W7A based on UniProtKB P23909.
ProteinModelPortal	Q6CSR1.
ModBase	Search...
Protein-protein interaction databases
STRING	28985.Q6CSR1.
Proteomic databases
PRIDE	Q6CSR1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2892153.
KEGG	kla:KLLA0C18590g.
Phylogenomic databases
eggNOG	COG0249.
HOGENOM	HOG000057130.
KO	K08736.
OMA	KRTKSIY.
OrthoDB	EOG42NN7M.
Family and domain databases
Gene3D	3.40.1170.10. 1 hit.
InterPro	IPR007695. DNA_mismatch_repair_MutS-lik_N. IPR000432. DNA_mismatch_repair_MutS_C. IPR007861. DNA_mismatch_repair_MutS_clamp. IPR007696. DNA_mismatch_repair_MutS_core. IPR016151. DNA_mismatch_repair_MutS_N. IPR007860. DNA_mmatch_repair_MutS_con_dom. [Graphical view]
Pfam	PF01624. MutS_I. 1 hit. PF05188. MutS_II. 1 hit. PF05192. MutS_III. 1 hit. PF05190. MutS_IV. 1 hit. PF00488. MutS_V. 1 hit. [Graphical view]
SMART	SM00534. MUTSac. 1 hit. SM00533. MUTSd. 1 hit. [Graphical view]
SUPFAM	SSF55271. DNA_mismatch_repair_MutS_N. 1 hit. SSF48334. DNA_repair_MutS_domIII. 1 hit.
PROSITE	PS00486. DNA_MISMATCH_REPAIR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MSH3_KLULA

Accession

Primary (citable) accession number: Q6CSR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program