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Q6CSH0 (DNLI4_KLULA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase 4

EC=6.5.1.1
Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4
Gene names
Name:LIG4
Ordered Locus Names:KLLA0D01089g
OrganismKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) [Complete proteome]
Taxonomic identifier284590 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces

Protein attributes

Sequence length907 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity.

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactor

Magnesium By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Contains 2 BRCT domains.

Ontologies

Keywords
   Biological processDNA damage
DNA recombination
DNA repair
DNA replication
   Cellular componentNucleus
   DomainRepeat
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA recombination

Inferred from electronic annotation. Source: UniProtKB-KW

DNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

DNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA binding

Inferred from electronic annotation. Source: InterPro

DNA ligase (ATP) activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 907907DNA ligase 4
PRO_0000278383

Regions

Domain655 – 754100BRCT 1
Domain800 – 906107BRCT 2

Sites

Active site2751N6-AMP-lysine intermediate By similarity
Metal binding3331Magnesium 1 Potential
Metal binding4381Magnesium 2 Potential
Binding site2731ATP By similarity
Binding site2801ATP By similarity
Binding site2971ATP By similarity
Binding site4431ATP By similarity
Binding site4541ATP By similarity
Binding site4601ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6CSH0 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 1CE0987C0B4ADFDE

FASTA907104,634
        10         20         30         40         50         60 
MNDCENFSPS PEFKWLCDEL LGKIYETSSK KHLIGKPVTV RYLEIITNFI KLWRSTVGNY 

        70         80         90        100        110        120 
IYPALRLIVP FRDRRIYNVK ENTLIKALCR YLRLPKSSET ENRLLRWKQR AARGVKLSDF 

       130        140        150        160        170        180 
CVEEIRKRQK DYEGANRITI DELNGYLDEV SQEGNGKRMG YMALTDSRAF NYCLNHMTFM 

       190        200        210        220        230        240 
EMKFFFDIIL KTRVLSGLEN MFLTAWHPDA TDYLSVVSDL DVLSQRLYNP NERLRQTDLS 

       250        260        270        280        290        300 
ITISHAFEPQ LAKRTHLSYE RVASKLQHDF IIEEKMDGER LQIHYINYGE QIKYLSRRGV 

       310        320        330        340        350        360 
DFSYLYGENS SSGPISPSLK LHFNVKDCIL DGEMITYDTE KDIVLPFGLV KSSAMNQIQS 

       370        380        390        400        410        420 
ELAGIAPTES YKPLFVAFDL VYLNGKSLTN LALERRKDYL TKILTPVERS VEIIQYMKAI 

       430        440        450        460        470        480 
NAEAIKDSLE QAISMGSEGI VLKHLHSKYF VGSRNTDWIK IKPEYLEQFG ENMDLLIIGR 

       490        500        510        520        530        540 
EQGKKDSFFC GLSISDPNEV AEKPRFISFC TIANGLSNEE FKDIERKTWG KWHIFSEDPP 

       550        560        570        580        590        600 
SPNLLGFGTK VPYEWIHPED SVVLEVKARA IDTKESEKRK YRSGCTLHFG YCKQIRYDKD 

       610        620        630        640        650        660 
WKTVASFSEF EDMKDARNFY NKRKSHQVTD GKKRASKRAK IGIVNSSEPT ALVAPVSNTF 

       670        680        690        700        710        720 
SNCRFRVISD YFDSTKRRRI SQEDLCSVIL EHGGEIVYTS DENNLPQDNL YIIGEKLTRE 

       730        740        750        760        770        780 
CKILLNAKNL IIRPSWIFSC IEEGYKTPFT ESDIFRGELE SSMDCSQFYT DFNTASLNHL 

       790        800        810        820        830        840 
LETANRGIKN PDSDLLLPEV PLFLFSNLKL AVLNSENVLD TSILEVEFAI KCHGGELVHI 

       850        860        870        880        890        900 
ENASIIIVFN DFISREDLFS LRQKIASKAV KESTESTPRI PRMVDISWAL DSIKDNYIAE 


TEHYQCL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR382124 Genomic DNA. Translation: CAH00215.1.
RefSeqXP_453119.1. XM_453119.1.

3D structure databases

ProteinModelPortalQ6CSH0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING28985.Q6CSH0.

Proteomic databases

PRIDEQ6CSH0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2892937.
KEGGkla:KLLA0D01089g.

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000093622.
KOK10777.
OMAKMDGDRM.
OrthoDBEOG7QC84G.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
3.40.50.10190. 1 hit.
InterProIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMSSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS50172. BRCT. 2 hits.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI4_KLULA
AccessionPrimary (citable) accession number: Q6CSH0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: August 16, 2004
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families