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Protein

DNA ligase 4

Gene

LIG4

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining.By similarity

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei273ATPBy similarity1
Active sitei275N6-AMP-lysine intermediateBy similarity1
Binding sitei280ATPBy similarity1
Binding sitei297ATPBy similarity1
Metal bindingi333Magnesium 1Sequence analysis1
Metal bindingi438Magnesium 2Sequence analysis1
Binding sitei443ATPBy similarity1
Binding sitei454ATPBy similarity1
Binding sitei460ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 4 (EC:6.5.1.1)
Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4
Gene namesi
Name:LIG4
Ordered Locus Names:KLLA0D01089g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome D

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002783831 – 907DNA ligase 4Add BLAST907

Proteomic databases

PRIDEiQ6CSH0.

Interactioni

Protein-protein interaction databases

STRINGi284590.XP_453119.1.

Structurei

3D structure databases

ProteinModelPortaliQ6CSH0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini655 – 754BRCT 1PROSITE-ProRule annotationAdd BLAST100
Domaini800 – 906BRCT 2PROSITE-ProRule annotationAdd BLAST107

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.Curated
Contains 2 BRCT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0966. Eukaryota.
COG1793. LUCA.
HOGENOMiHOG000093622.
InParanoidiQ6CSH0.
KOiK10777.
OMAiFCTIANG.
OrthoDBiEOG092C18KW.

Family and domain databases

Gene3Di3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR012308. DNA_ligase_ATP-dep_N.
IPR029710. LIG4.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PANTHERiPTHR10459:SF7. PTHR10459:SF7. 1 hit.
PfamiPF16589. BRCT_2. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS50172. BRCT. 2 hits.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6CSH0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDCENFSPS PEFKWLCDEL LGKIYETSSK KHLIGKPVTV RYLEIITNFI
60 70 80 90 100
KLWRSTVGNY IYPALRLIVP FRDRRIYNVK ENTLIKALCR YLRLPKSSET
110 120 130 140 150
ENRLLRWKQR AARGVKLSDF CVEEIRKRQK DYEGANRITI DELNGYLDEV
160 170 180 190 200
SQEGNGKRMG YMALTDSRAF NYCLNHMTFM EMKFFFDIIL KTRVLSGLEN
210 220 230 240 250
MFLTAWHPDA TDYLSVVSDL DVLSQRLYNP NERLRQTDLS ITISHAFEPQ
260 270 280 290 300
LAKRTHLSYE RVASKLQHDF IIEEKMDGER LQIHYINYGE QIKYLSRRGV
310 320 330 340 350
DFSYLYGENS SSGPISPSLK LHFNVKDCIL DGEMITYDTE KDIVLPFGLV
360 370 380 390 400
KSSAMNQIQS ELAGIAPTES YKPLFVAFDL VYLNGKSLTN LALERRKDYL
410 420 430 440 450
TKILTPVERS VEIIQYMKAI NAEAIKDSLE QAISMGSEGI VLKHLHSKYF
460 470 480 490 500
VGSRNTDWIK IKPEYLEQFG ENMDLLIIGR EQGKKDSFFC GLSISDPNEV
510 520 530 540 550
AEKPRFISFC TIANGLSNEE FKDIERKTWG KWHIFSEDPP SPNLLGFGTK
560 570 580 590 600
VPYEWIHPED SVVLEVKARA IDTKESEKRK YRSGCTLHFG YCKQIRYDKD
610 620 630 640 650
WKTVASFSEF EDMKDARNFY NKRKSHQVTD GKKRASKRAK IGIVNSSEPT
660 670 680 690 700
ALVAPVSNTF SNCRFRVISD YFDSTKRRRI SQEDLCSVIL EHGGEIVYTS
710 720 730 740 750
DENNLPQDNL YIIGEKLTRE CKILLNAKNL IIRPSWIFSC IEEGYKTPFT
760 770 780 790 800
ESDIFRGELE SSMDCSQFYT DFNTASLNHL LETANRGIKN PDSDLLLPEV
810 820 830 840 850
PLFLFSNLKL AVLNSENVLD TSILEVEFAI KCHGGELVHI ENASIIIVFN
860 870 880 890 900
DFISREDLFS LRQKIASKAV KESTESTPRI PRMVDISWAL DSIKDNYIAE

TEHYQCL
Length:907
Mass (Da):104,634
Last modified:August 16, 2004 - v1
Checksum:i1CE0987C0B4ADFDE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382124 Genomic DNA. Translation: CAH00215.1.
RefSeqiXP_453119.1. XM_453119.1.

Genome annotation databases

EnsemblFungiiCAH00215; CAH00215; KLLA0_D01089g.
GeneIDi2892937.
KEGGikla:KLLA0D01089g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382124 Genomic DNA. Translation: CAH00215.1.
RefSeqiXP_453119.1. XM_453119.1.

3D structure databases

ProteinModelPortaliQ6CSH0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi284590.XP_453119.1.

Proteomic databases

PRIDEiQ6CSH0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAH00215; CAH00215; KLLA0_D01089g.
GeneIDi2892937.
KEGGikla:KLLA0D01089g.

Phylogenomic databases

eggNOGiKOG0966. Eukaryota.
COG1793. LUCA.
HOGENOMiHOG000093622.
InParanoidiQ6CSH0.
KOiK10777.
OMAiFCTIANG.
OrthoDBiEOG092C18KW.

Family and domain databases

Gene3Di3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR012308. DNA_ligase_ATP-dep_N.
IPR029710. LIG4.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PANTHERiPTHR10459:SF7. PTHR10459:SF7. 1 hit.
PfamiPF16589. BRCT_2. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS50172. BRCT. 2 hits.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNLI4_KLULA
AccessioniPrimary (citable) accession number: Q6CSH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: August 16, 2004
Last modified: November 30, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.