Reviewed, UniProtKB/Swiss-Prot Q6CSH0 (DNLI4_KLULA)

Names and origin

Protein names

Recommended name:
    DNA ligase 4
    EC=6.5.1.1
Alternative name(s):
    DNA ligase IV
    Polydeoxyribonucleotide synthase [ATP] 4

Gene names

Name:	LIG4
Ordered Locus Names:	KLLA0D01089g

Organism

Kluyveromyces lactis (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

28985 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	907 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Subcellular location	NucleusBy similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family. Contains 2 BRCT domains.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: InterPro DNA repair Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 907	907	DNA ligase 4		PRO_0000278383
Regions
	Domain	655 – 754	100	BRCT 1
	Domain	800 – 906	107	BRCT 2
Sites
	Active site	275	1	N6-AMP-lysine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6CSH0-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 1CE0987C0B4ADFDE	FASTA	907	104,634
	10 20 30 40 50 60 MNDCENFSPS PEFKWLCDEL LGKIYETSSK KHLIGKPVTV RYLEIITNFI KLWRSTVGNY 70 80 90 100 110 120 IYPALRLIVP FRDRRIYNVK ENTLIKALCR YLRLPKSSET ENRLLRWKQR AARGVKLSDF 130 140 150 160 170 180 CVEEIRKRQK DYEGANRITI DELNGYLDEV SQEGNGKRMG YMALTDSRAF NYCLNHMTFM 190 200 210 220 230 240 EMKFFFDIIL KTRVLSGLEN MFLTAWHPDA TDYLSVVSDL DVLSQRLYNP NERLRQTDLS 250 260 270 280 290 300 ITISHAFEPQ LAKRTHLSYE RVASKLQHDF IIEEKMDGER LQIHYINYGE QIKYLSRRGV 310 320 330 340 350 360 DFSYLYGENS SSGPISPSLK LHFNVKDCIL DGEMITYDTE KDIVLPFGLV KSSAMNQIQS 370 380 390 400 410 420 ELAGIAPTES YKPLFVAFDL VYLNGKSLTN LALERRKDYL TKILTPVERS VEIIQYMKAI 430 440 450 460 470 480 NAEAIKDSLE QAISMGSEGI VLKHLHSKYF VGSRNTDWIK IKPEYLEQFG ENMDLLIIGR 490 500 510 520 530 540 EQGKKDSFFC GLSISDPNEV AEKPRFISFC TIANGLSNEE FKDIERKTWG KWHIFSEDPP 550 560 570 580 590 600 SPNLLGFGTK VPYEWIHPED SVVLEVKARA IDTKESEKRK YRSGCTLHFG YCKQIRYDKD 610 620 630 640 650 660 WKTVASFSEF EDMKDARNFY NKRKSHQVTD GKKRASKRAK IGIVNSSEPT ALVAPVSNTF 670 680 690 700 710 720 SNCRFRVISD YFDSTKRRRI SQEDLCSVIL EHGGEIVYTS DENNLPQDNL YIIGEKLTRE 730 740 750 760 770 780 CKILLNAKNL IIRPSWIFSC IEEGYKTPFT ESDIFRGELE SSMDCSQFYT DFNTASLNHL 790 800 810 820 830 840 LETANRGIKN PDSDLLLPEV PLFLFSNLKL AVLNSENVLD TSILEVEFAI KCHGGELVHI 850 860 870 880 890 900 ENASIIIVFN DFISREDLFS LRQKIASKAV KESTESTPRI PRMVDISWAL DSIKDNYIAE TEHYQCL

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR382124 Genomic DNA. Translation: CAH00215.1.
RefSeq	XP_453119.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2892937.
KEGG	kla:KLLA0D01089g.
Phylogenomic databases
HOGENOM	Q6CSH0.
Family and domain databases
InterPro	IPR001357. BRCT. IPR000977. DNA_ligase. IPR012310. DNA_ligase_A_M. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS50172. BRCT. 2 hits. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI4_KLULA

Accession

Primary (citable) accession number: Q6CSH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	August 16, 2004
Last modified:	November 25, 2008
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)