Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q6CSH0

- DNLI4_KLULA

UniProt

Q6CSH0 - DNLI4_KLULA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

DNA ligase 4

Gene

LIG4

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining (By similarity).By similarity

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactori

Magnesium.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei273 – 2731ATPBy similarity
Active sitei275 – 2751N6-AMP-lysine intermediateBy similarity
Binding sitei280 – 2801ATPBy similarity
Binding sitei297 – 2971ATPBy similarity
Metal bindingi333 – 3331Magnesium 1Sequence Analysis
Metal bindingi438 – 4381Magnesium 2Sequence Analysis
Binding sitei443 – 4431ATPBy similarity
Binding sitei454 – 4541ATPBy similarity
Binding sitei460 – 4601ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA biosynthetic process Source: InterPro
  2. DNA ligation involved in DNA repair Source: InterPro
  3. DNA recombination Source: UniProtKB-KW
  4. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 4 (EC:6.5.1.1)
Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4
Gene namesi
Name:LIG4
Ordered Locus Names:KLLA0D01089g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598: Chromosome D

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 907907DNA ligase 4PRO_0000278383Add
BLAST

Proteomic databases

PRIDEiQ6CSH0.

Interactioni

Protein-protein interaction databases

STRINGi28985.Q6CSH0.

Structurei

3D structure databases

ProteinModelPortaliQ6CSH0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini655 – 754100BRCT 1PROSITE-ProRule annotationAdd
BLAST
Domaini800 – 906107BRCT 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.Curated
Contains 2 BRCT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1793.
HOGENOMiHOG000093622.
InParanoidiQ6CSH0.
KOiK10777.
OMAiKMDGDRM.
OrthoDBiEOG7QC84G.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR012308. DNA_ligase_ATP-dep_N.
IPR029710. LIG4.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PANTHERiPTHR10459:SF7. PTHR10459:SF7. 1 hit.
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS50172. BRCT. 2 hits.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6CSH0 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNDCENFSPS PEFKWLCDEL LGKIYETSSK KHLIGKPVTV RYLEIITNFI
60 70 80 90 100
KLWRSTVGNY IYPALRLIVP FRDRRIYNVK ENTLIKALCR YLRLPKSSET
110 120 130 140 150
ENRLLRWKQR AARGVKLSDF CVEEIRKRQK DYEGANRITI DELNGYLDEV
160 170 180 190 200
SQEGNGKRMG YMALTDSRAF NYCLNHMTFM EMKFFFDIIL KTRVLSGLEN
210 220 230 240 250
MFLTAWHPDA TDYLSVVSDL DVLSQRLYNP NERLRQTDLS ITISHAFEPQ
260 270 280 290 300
LAKRTHLSYE RVASKLQHDF IIEEKMDGER LQIHYINYGE QIKYLSRRGV
310 320 330 340 350
DFSYLYGENS SSGPISPSLK LHFNVKDCIL DGEMITYDTE KDIVLPFGLV
360 370 380 390 400
KSSAMNQIQS ELAGIAPTES YKPLFVAFDL VYLNGKSLTN LALERRKDYL
410 420 430 440 450
TKILTPVERS VEIIQYMKAI NAEAIKDSLE QAISMGSEGI VLKHLHSKYF
460 470 480 490 500
VGSRNTDWIK IKPEYLEQFG ENMDLLIIGR EQGKKDSFFC GLSISDPNEV
510 520 530 540 550
AEKPRFISFC TIANGLSNEE FKDIERKTWG KWHIFSEDPP SPNLLGFGTK
560 570 580 590 600
VPYEWIHPED SVVLEVKARA IDTKESEKRK YRSGCTLHFG YCKQIRYDKD
610 620 630 640 650
WKTVASFSEF EDMKDARNFY NKRKSHQVTD GKKRASKRAK IGIVNSSEPT
660 670 680 690 700
ALVAPVSNTF SNCRFRVISD YFDSTKRRRI SQEDLCSVIL EHGGEIVYTS
710 720 730 740 750
DENNLPQDNL YIIGEKLTRE CKILLNAKNL IIRPSWIFSC IEEGYKTPFT
760 770 780 790 800
ESDIFRGELE SSMDCSQFYT DFNTASLNHL LETANRGIKN PDSDLLLPEV
810 820 830 840 850
PLFLFSNLKL AVLNSENVLD TSILEVEFAI KCHGGELVHI ENASIIIVFN
860 870 880 890 900
DFISREDLFS LRQKIASKAV KESTESTPRI PRMVDISWAL DSIKDNYIAE

TEHYQCL
Length:907
Mass (Da):104,634
Last modified:August 16, 2004 - v1
Checksum:i1CE0987C0B4ADFDE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR382124 Genomic DNA. Translation: CAH00215.1.
RefSeqiXP_453119.1. XM_453119.1.

Genome annotation databases

GeneIDi2892937.
KEGGikla:KLLA0D01089g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR382124 Genomic DNA. Translation: CAH00215.1 .
RefSeqi XP_453119.1. XM_453119.1.

3D structure databases

ProteinModelPortali Q6CSH0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 28985.Q6CSH0.

Proteomic databases

PRIDEi Q6CSH0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2892937.
KEGGi kla:KLLA0D01089g.

Phylogenomic databases

eggNOGi COG1793.
HOGENOMi HOG000093622.
InParanoidi Q6CSH0.
KOi K10777.
OMAi KMDGDRM.
OrthoDBi EOG7QC84G.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
3.40.50.10190. 1 hit.
InterProi IPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR012308. DNA_ligase_ATP-dep_N.
IPR029710. LIG4.
IPR012340. NA-bd_OB-fold.
[Graphical view ]
PANTHERi PTHR10459:SF7. PTHR10459:SF7. 1 hit.
Pfami PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view ]
SMARTi SM00292. BRCT. 1 hit.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsi TIGR00574. dnl1. 1 hit.
PROSITEi PS50172. BRCT. 2 hits.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Entry informationi

Entry nameiDNLI4_KLULA
AccessioniPrimary (citable) accession number: Q6CSH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: August 16, 2004
Last modified: October 29, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3