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Q6CSH0

- DNLI4_KLULA

UniProt

Q6CSH0 - DNLI4_KLULA

Protein

DNA ligase 4

Gene

LIG4

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity.By similarity

    Catalytic activityi

    ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

    Cofactori

    Magnesium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei273 – 2731ATPBy similarity
    Active sitei275 – 2751N6-AMP-lysine intermediateBy similarity
    Binding sitei280 – 2801ATPBy similarity
    Binding sitei297 – 2971ATPBy similarity
    Metal bindingi333 – 3331Magnesium 1Sequence Analysis
    Metal bindingi438 – 4381Magnesium 2Sequence Analysis
    Binding sitei443 – 4431ATPBy similarity
    Binding sitei454 – 4541ATPBy similarity
    Binding sitei460 – 4601ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: InterPro
    3. DNA ligase (ATP) activity Source: UniProtKB-EC
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-KW
    2. DNA repair Source: UniProtKB-KW
    3. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair, DNA replication

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA ligase 4 (EC:6.5.1.1)
    Alternative name(s):
    DNA ligase IV
    Polydeoxyribonucleotide synthase [ATP] 4
    Gene namesi
    Name:LIG4
    Ordered Locus Names:KLLA0D01089g
    OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
    Taxonomic identifieri284590 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
    ProteomesiUP000000598: Chromosome D

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 907907DNA ligase 4PRO_0000278383Add
    BLAST

    Proteomic databases

    PRIDEiQ6CSH0.

    Interactioni

    Protein-protein interaction databases

    STRINGi28985.Q6CSH0.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6CSH0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini655 – 754100BRCT 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini800 – 906107BRCT 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATP-dependent DNA ligase family.Curated
    Contains 2 BRCT domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1793.
    HOGENOMiHOG000093622.
    KOiK10777.
    OMAiKMDGDRM.
    OrthoDBiEOG7QC84G.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.40.50.10190. 1 hit.
    InterProiIPR001357. BRCT_dom.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view]
    PfamiPF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view]
    SMARTiSM00292. BRCT. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF52113. SSF52113. 1 hit.
    TIGRFAMsiTIGR00574. dnl1. 1 hit.
    PROSITEiPS50172. BRCT. 2 hits.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6CSH0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNDCENFSPS PEFKWLCDEL LGKIYETSSK KHLIGKPVTV RYLEIITNFI    50
    KLWRSTVGNY IYPALRLIVP FRDRRIYNVK ENTLIKALCR YLRLPKSSET 100
    ENRLLRWKQR AARGVKLSDF CVEEIRKRQK DYEGANRITI DELNGYLDEV 150
    SQEGNGKRMG YMALTDSRAF NYCLNHMTFM EMKFFFDIIL KTRVLSGLEN 200
    MFLTAWHPDA TDYLSVVSDL DVLSQRLYNP NERLRQTDLS ITISHAFEPQ 250
    LAKRTHLSYE RVASKLQHDF IIEEKMDGER LQIHYINYGE QIKYLSRRGV 300
    DFSYLYGENS SSGPISPSLK LHFNVKDCIL DGEMITYDTE KDIVLPFGLV 350
    KSSAMNQIQS ELAGIAPTES YKPLFVAFDL VYLNGKSLTN LALERRKDYL 400
    TKILTPVERS VEIIQYMKAI NAEAIKDSLE QAISMGSEGI VLKHLHSKYF 450
    VGSRNTDWIK IKPEYLEQFG ENMDLLIIGR EQGKKDSFFC GLSISDPNEV 500
    AEKPRFISFC TIANGLSNEE FKDIERKTWG KWHIFSEDPP SPNLLGFGTK 550
    VPYEWIHPED SVVLEVKARA IDTKESEKRK YRSGCTLHFG YCKQIRYDKD 600
    WKTVASFSEF EDMKDARNFY NKRKSHQVTD GKKRASKRAK IGIVNSSEPT 650
    ALVAPVSNTF SNCRFRVISD YFDSTKRRRI SQEDLCSVIL EHGGEIVYTS 700
    DENNLPQDNL YIIGEKLTRE CKILLNAKNL IIRPSWIFSC IEEGYKTPFT 750
    ESDIFRGELE SSMDCSQFYT DFNTASLNHL LETANRGIKN PDSDLLLPEV 800
    PLFLFSNLKL AVLNSENVLD TSILEVEFAI KCHGGELVHI ENASIIIVFN 850
    DFISREDLFS LRQKIASKAV KESTESTPRI PRMVDISWAL DSIKDNYIAE 900
    TEHYQCL 907
    Length:907
    Mass (Da):104,634
    Last modified:August 16, 2004 - v1
    Checksum:i1CE0987C0B4ADFDE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR382124 Genomic DNA. Translation: CAH00215.1.
    RefSeqiXP_453119.1. XM_453119.1.

    Genome annotation databases

    GeneIDi2892937.
    KEGGikla:KLLA0D01089g.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR382124 Genomic DNA. Translation: CAH00215.1 .
    RefSeqi XP_453119.1. XM_453119.1.

    3D structure databases

    ProteinModelPortali Q6CSH0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 28985.Q6CSH0.

    Proteomic databases

    PRIDEi Q6CSH0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2892937.
    KEGGi kla:KLLA0D01089g.

    Phylogenomic databases

    eggNOGi COG1793.
    HOGENOMi HOG000093622.
    KOi K10777.
    OMAi KMDGDRM.
    OrthoDBi EOG7QC84G.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.40.50.10190. 1 hit.
    InterProi IPR001357. BRCT_dom.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view ]
    Pfami PF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view ]
    SMARTi SM00292. BRCT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF52113. SSF52113. 1 hit.
    TIGRFAMsi TIGR00574. dnl1. 1 hit.
    PROSITEi PS50172. BRCT. 2 hits.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Entry informationi

    Entry nameiDNLI4_KLULA
    AccessioniPrimary (citable) accession number: Q6CSH0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 20, 2007
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3