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Protein

DNA ligase 4

Gene

LIG4

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologous end-joining.By similarity

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei273ATPBy similarity1
Active sitei275N6-AMP-lysine intermediateBy similarity1
Binding sitei280ATPBy similarity1
Binding sitei297ATPBy similarity1
Metal bindingi333Magnesium 1Sequence analysis1
Metal bindingi438Magnesium 2Sequence analysis1
Binding sitei443ATPBy similarity1
Binding sitei454ATPBy similarity1
Binding sitei460ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processDNA damage, DNA recombination, DNA repair, DNA replication
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 4 (EC:6.5.1.1)
Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4
Gene namesi
Name:LIG4
Ordered Locus Names:KLLA0D01089g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome D

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002783831 – 907DNA ligase 4Add BLAST907

Proteomic databases

PRIDEiQ6CSH0

Interactioni

Protein-protein interaction databases

STRINGi284590.XP_453119.1

Structurei

3D structure databases

ProteinModelPortaliQ6CSH0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini655 – 754BRCT 1PROSITE-ProRule annotationAdd BLAST100
Domaini800 – 906BRCT 2PROSITE-ProRule annotationAdd BLAST107

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0966 Eukaryota
COG1793 LUCA
HOGENOMiHOG000093622
InParanoidiQ6CSH0
KOiK10777
OMAiHMCPSTK
OrthoDBiEOG092C18KW

Family and domain databases

Gene3Di1.10.3260.101 hit
3.40.50.101902 hits
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR000977 DNA_ligase_ATP-dep
IPR012310 DNA_ligase_ATP-dep_cent
IPR012308 DNA_ligase_ATP-dep_N
IPR036599 DNA_ligase_N_sf
IPR029710 LIG4
IPR012340 NA-bd_OB-fold
PANTHERiPTHR10459:SF7 PTHR10459:SF7, 1 hit
PfamiView protein in Pfam
PF16589 BRCT_2, 1 hit
PF01068 DNA_ligase_A_M, 1 hit
PF04675 DNA_ligase_A_N, 1 hit
SMARTiView protein in SMART
SM00292 BRCT, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52113 SSF52113, 1 hit
TIGRFAMsiTIGR00574 dnl1, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 2 hits
PS50160 DNA_LIGASE_A3, 1 hit

Sequencei

Sequence statusi: Complete.

Q6CSH0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDCENFSPS PEFKWLCDEL LGKIYETSSK KHLIGKPVTV RYLEIITNFI
60 70 80 90 100
KLWRSTVGNY IYPALRLIVP FRDRRIYNVK ENTLIKALCR YLRLPKSSET
110 120 130 140 150
ENRLLRWKQR AARGVKLSDF CVEEIRKRQK DYEGANRITI DELNGYLDEV
160 170 180 190 200
SQEGNGKRMG YMALTDSRAF NYCLNHMTFM EMKFFFDIIL KTRVLSGLEN
210 220 230 240 250
MFLTAWHPDA TDYLSVVSDL DVLSQRLYNP NERLRQTDLS ITISHAFEPQ
260 270 280 290 300
LAKRTHLSYE RVASKLQHDF IIEEKMDGER LQIHYINYGE QIKYLSRRGV
310 320 330 340 350
DFSYLYGENS SSGPISPSLK LHFNVKDCIL DGEMITYDTE KDIVLPFGLV
360 370 380 390 400
KSSAMNQIQS ELAGIAPTES YKPLFVAFDL VYLNGKSLTN LALERRKDYL
410 420 430 440 450
TKILTPVERS VEIIQYMKAI NAEAIKDSLE QAISMGSEGI VLKHLHSKYF
460 470 480 490 500
VGSRNTDWIK IKPEYLEQFG ENMDLLIIGR EQGKKDSFFC GLSISDPNEV
510 520 530 540 550
AEKPRFISFC TIANGLSNEE FKDIERKTWG KWHIFSEDPP SPNLLGFGTK
560 570 580 590 600
VPYEWIHPED SVVLEVKARA IDTKESEKRK YRSGCTLHFG YCKQIRYDKD
610 620 630 640 650
WKTVASFSEF EDMKDARNFY NKRKSHQVTD GKKRASKRAK IGIVNSSEPT
660 670 680 690 700
ALVAPVSNTF SNCRFRVISD YFDSTKRRRI SQEDLCSVIL EHGGEIVYTS
710 720 730 740 750
DENNLPQDNL YIIGEKLTRE CKILLNAKNL IIRPSWIFSC IEEGYKTPFT
760 770 780 790 800
ESDIFRGELE SSMDCSQFYT DFNTASLNHL LETANRGIKN PDSDLLLPEV
810 820 830 840 850
PLFLFSNLKL AVLNSENVLD TSILEVEFAI KCHGGELVHI ENASIIIVFN
860 870 880 890 900
DFISREDLFS LRQKIASKAV KESTESTPRI PRMVDISWAL DSIKDNYIAE

TEHYQCL
Length:907
Mass (Da):104,634
Last modified:August 16, 2004 - v1
Checksum:i1CE0987C0B4ADFDE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382124 Genomic DNA Translation: CAH00215.1
RefSeqiXP_453119.1, XM_453119.1

Genome annotation databases

EnsemblFungiiCAH00215; CAH00215; KLLA0_D01089g
GeneIDi2892937
KEGGikla:KLLA0D01089g

Similar proteinsi

Entry informationi

Entry nameiDNLI4_KLULA
AccessioniPrimary (citable) accession number: Q6CSH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: August 16, 2004
Last modified: February 28, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome