Reviewed, UniProtKB/Swiss-Prot Q6CRA9 (BUR1_KLULA)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase BUR1
    EC=2.7.11.22
    EC=2.7.11.23

Gene names

Name:	BUR1
Ordered Locus Names:	KLLA0D10527g

Organism

Kluyveromyces lactis (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

28985 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	645 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Serine/threonine-protein kinase involved in transcription regulation. Phosphorylates the UBC2/RAD6 ubiquitin-conjugating enzyme (E2), leading to monoubiquitination of histone H2B and the silencing of telomeric-associated genes. Also required for histone H3 methylation. Necessary for the recovery from pheromone-induced growth arrest in the cell cycle G1 phase By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 645	645	Serine/threonine-protein kinase BUR1		PRO_0000085684
Regions
	Domain	55 – 379	325	Protein kinase
	Nucleotide binding	61 – 69	9	ATP By similarity
Sites
	Active site	208	1	Proton acceptor By similarity
	Binding site	84	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6CRA9-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: DB586D142DD0FBC8	FASTA	645	72,214
	10 20 30 40 50 60 MSTVNKKPAS LKYKIGKVKQ IPTVVKDDKT GLEYIQVQSR ENEKVYGVTK FLNNYREEEK 70 80 90 100 110 120 LGQGTFGEVF KGIHLGTNRK VAIKRILVRA EKDLFPITAQ REITILKRMN HKNIVKLIEI 130 140 150 160 170 180 VYDESPTPKT DSTSPRPVGN YHGNNTANQQ KLITGKHFFM ILPYMVSDLT GLLHNPRVEF 190 200 210 220 230 240 GMADVKNIML QLLEGINYIH CNKFLHRDIK TANILIDHKG VVKIADFGLA RNYYGSPPNL 250 260 270 280 290 300 KYPGGAGSGA KYTSVVVTRW YRAPEIVLGD RHYTTAVDIW GIGCVFAEFF EKKPILQGQT 310 320 330 340 350 360 DIDQGHVIFK LMGTPSMDEW GLAYHLPGSE LTKTNYKSTL QERFSKALNE TGLDLLSKLL 370 380 390 400 410 420 ALDPYKRVTA MKAKKHPFFF EEPLPNAQLT LPNEECHESD IKRYKSEMNE SMSQRPPSAP 430 440 450 460 470 480 TGHTSTDNSI RAVTGAAFPK EASIPKAPRP EHIKSTIPAQ PSASRYNGAA TQNIPKEPIP 490 500 510 520 530 540 TAPLPKGPKN SIPTGPNKLP PNPRDSYASK YPAESRFGVN TRITTESYNA GKRYRNRGGW 550 560 570 580 590 600 ETGRDSLNYN NNYAPPADRS HHPTIQRPSA PRGGSYPNRY QNQDYNTSRN TGYNQYSQAG 610 620 630 640 DRRSYRPGAS ENPGTIGHGS SQSTQKDSKS SHSNNVKPKD VADYY

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382124 Genomic DNA. Translation: CAH00626.1.
RefSeq	XP_453530.1.
3D structure databases
SMR	Q6CRA9. Positions 51-417.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6CRA9.
Genome annotation databases
GeneID	2893554.
GenomeReviews	Gene locus KLLA0D10527g in contig CR382124_GR.
KEGG	kla:KLLA0D10527g.
Phylogenomic databases
eggNOG	fuNOG04620.
HOGENOM	HBG204009.
OMA	NEECHES.
OrthoDB	EOG96T4J6.
PhylomeDB	Q6CRA9.
Enzyme and pathway databases
BRENDA	2.7.11.22. 74088. 2.7.11.23. 74088.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BUR1_KLULA

Accession

Primary (citable) accession number: Q6CRA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	August 16, 2004
Last modified:	January 19, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)