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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

DUT1

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.By similarity

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

Cofactori

Mg2+By similarity

Pathwayi: dUMP biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (DUT1)
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei93Substrate; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei137SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
dUTP pyrophosphatase
Gene namesi
Name:DUT1
Ordered Locus Names:KLLA0D15697g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome D

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001829331 – 148Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST148

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi284590.XP_453752.1

Structurei

3D structure databases

ProteinModelPortaliQ6CQN7
SMRiQ6CQN7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni68 – 70Substrate bindingBy similarity3
Regioni82 – 85Substrate bindingBy similarity4
Regioni142 – 143Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the dUTPase family.Curated

Phylogenomic databases

eggNOGiKOG3370 Eukaryota
COG0756 LUCA
HOGENOMiHOG000028966
InParanoidiQ6CQN7
KOiK01520
OMAiGVILINH
OrthoDBiEOG092C5PPT

Family and domain databases

CDDicd07557 trimeric_dUTPase, 1 hit
Gene3Di2.70.40.101 hit
InterProiView protein in InterPro
IPR029054 dUTPase-like
IPR036157 dUTPase-like_sf
IPR008181 dUTPase_1
IPR033704 dUTPase_trimeric
PfamiView protein in Pfam
PF00692 dUTPase, 1 hit
SUPFAMiSSF51283 SSF51283, 1 hit
TIGRFAMsiTIGR00576 dut, 1 hit

Sequencei

Sequence statusi: Complete.

Q6CQN7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTESLSNSLK VQLRSADAKV PTKGSTTAAG YDIYASQPGV IPARGQGIAK
60 70 80 90 100
TDISFTVPVG TYGRIAPRSG LAVKHGIQTG AGVVDRDYTG EVGIVLFNHS
110 120 130 140
DKDFQINKGD RVAQLILEKI VEDAEIVVVE SLEETQRGAG GFGSTGKN
Length:148
Mass (Da):15,534
Last modified:August 16, 2004 - v1
Checksum:iE835B04A810A3DD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382124 Genomic DNA Translation: CAH00848.1
RefSeqiXP_453752.1, XM_453752.1

Genome annotation databases

EnsemblFungiiCAH00848; CAH00848; KLLA0_D15697g
GeneIDi2893442
KEGGikla:KLLA0D15697g

Similar proteinsi

Entry informationi

Entry nameiDUT_KLULA
AccessioniPrimary (citable) accession number: Q6CQN7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: August 16, 2004
Last modified: February 28, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome