Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Palmitoyltransferase PFA3

Gene

PFA3

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Palmitoyltransferase specific for VAC8. Palmitoylates VAC8 at one or more of its N-terminal cysteine residues, which is required for its proper membrane localization (By similarity).By similarity

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Palmitoyltransferase PFA3 (EC:2.3.1.225)
Alternative name(s):
Protein fatty acyltransferase 3
Gene namesi
Name:PFA3
Ordered Locus Names:KLLA0E02068g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome E

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 5CytoplasmicSequence analysis5
Transmembranei6 – 26HelicalSequence analysisAdd BLAST21
Topological domaini27 – 35LumenalSequence analysis9
Transmembranei36 – 56HelicalSequence analysisAdd BLAST21
Topological domaini57 – 146CytoplasmicSequence analysisAdd BLAST90
Transmembranei147 – 167HelicalSequence analysisAdd BLAST21
Topological domaini168 – 185LumenalSequence analysisAdd BLAST18
Transmembranei186 – 206HelicalSequence analysisAdd BLAST21
Topological domaini207 – 325CytoplasmicSequence analysisAdd BLAST119

GO - Cellular componenti

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002129561 – 325Palmitoyltransferase PFA3Add BLAST325

Post-translational modificationi

Autopalmitoylated.By similarity

Keywords - PTMi

Lipoprotein, Palmitate

Interactioni

Protein-protein interaction databases

STRINGi284590.XP_454041.1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini103 – 153DHHCPROSITE-ProRule annotationAdd BLAST51

Domaini

The DHHC domain is required for palmitoyltransferase activity.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1315. Eukaryota.
COG5273. LUCA.
HOGENOMiHOG000246777.
InParanoidiQ6CPU8.
KOiK20028.
OMAiHHCPWFA.
OrthoDBiEOG092C524D.

Family and domain databases

InterProiView protein in InterPro
IPR001594. Palmitoyltrfase_DHHC.
PfamiView protein in Pfam
PF01529. DHHC. 1 hit.
PROSITEiView protein in PROSITE
PS50216. DHHC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6CPU8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLDRIGFYF PKALSNFLIL YTCCICFSRI DILPRIVNVV LLITLSSFAL
60 70 80 90 100
YTYWKIIRVG AGSPLEYSFL KIQSIDNVLN RTEQPPDIIK DNCIFVKRDG
110 120 130 140 150
SFRFCQTCEI WKPDRCHHCS KCNKCFLKMD HHCPWFASCV GFRNQKFFVQ
160 170 180 190 200
FLAYTTVYSL YVLLMTSAQL YSWFRQMKYK SELLDLHLLV VWVLSVIAAI
210 220 230 240 250
ATFAFTTYTI WLVTKNETTI EQYEWGNIRH DLEIYGDSIN CNMGSVDNVF
260 270 280 290 300
DLGSRSANFN CVMGASWAEL LLPIQVRADD PFDPYANQGL FFPVQSDTYR
310 320
IYRESVNLQQ RLITRLTLRP SIEHI
Length:325
Mass (Da):37,933
Last modified:August 16, 2004 - v1
Checksum:iEB814ED28147DE97
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382125 Genomic DNA. Translation: CAG99128.1.
RefSeqiXP_454041.1. XM_454041.1.

Genome annotation databases

EnsemblFungiiCAG99128; CAG99128; KLLA0_E02069g.
GeneIDi2894120.
KEGGikla:KLLA0E02069g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382125 Genomic DNA. Translation: CAG99128.1.
RefSeqiXP_454041.1. XM_454041.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi284590.XP_454041.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAG99128; CAG99128; KLLA0_E02069g.
GeneIDi2894120.
KEGGikla:KLLA0E02069g.

Phylogenomic databases

eggNOGiKOG1315. Eukaryota.
COG5273. LUCA.
HOGENOMiHOG000246777.
InParanoidiQ6CPU8.
KOiK20028.
OMAiHHCPWFA.
OrthoDBiEOG092C524D.

Family and domain databases

InterProiView protein in InterPro
IPR001594. Palmitoyltrfase_DHHC.
PfamiView protein in Pfam
PF01529. DHHC. 1 hit.
PROSITEiView protein in PROSITE
PS50216. DHHC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPFA3_KLULA
AccessioniPrimary (citable) accession number: Q6CPU8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: August 16, 2004
Last modified: June 7, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.