Q6CNT6 (PP4C_KLULA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 4 catalytic subunit
Short name=PP4C
EC=3.1.3.16

Gene names

Name:	PPH3
Ordered Locus Names:	KLLA0E10032g

Organism

Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

284590 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces › Kluyveromyces lactis

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Forms the histone H2A phosphatase complex in association with the regulatory subunits PSY2 and PSY4, which dephosphorylates H2AS128ph (gamma-H2A) that has been displaced from sites of DNA lesions in the double-stranded DNA break repair process. Dephosphorylation is necessary for efficient recovery from the DNA damage checkpoint By similarity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Subunit structure	Catalytic subunit of the histone H2A phosphatase complex (HTP-C) containing PPH3, PSY2 and PSY4 By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
PTM	Methylation
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Serine/threonine-protein phosphatase 4 catalytic subunit		PRO_0000223651
Sites
	Active site	112	1	Proton donor By similarity
	Metal binding	51	1	Iron By similarity
	Metal binding	53	1	Iron By similarity
	Metal binding	79	1	Iron By similarity
	Metal binding	79	1	Manganese By similarity
	Metal binding	111	1	Manganese By similarity
	Metal binding	161	1	Manganese By similarity
	Metal binding	235	1	Manganese By similarity
Amino acid modifications
	Modified residue	308	1	Leucine methyl ester By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6CNT6 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 5BF6D3BFDAB84CD8	FASTA	308	35,167
	10 20 30 40 50 60 MMKLDEIIET LRQGKHVDED SIYSLCVMAQ ELLMNESNVT HVDTPVTICG DIHGQLHDLL 70 80 90 100 110 120 TLFAKSGGIE KNRYIFLGDF VDRGFYSLES FLLLVCYKLR YPDRIVLIRG NHETRQITKV 130 140 150 160 170 180 YGFYDEVVRK YGNSNVWRYC CEVFDYLPLG AIVNNKVFCV HGGLSPDVLS INEIRTIDRK 190 200 210 220 230 240 KEVPHEGAMC DLLWSDPEDV DTWSLSPRGA GFLFGQNEVD KFLHTNSVEL IARAHQLVME 250 260 270 280 290 300 GYKEMFDGGL VTVWSAPNYC YRCGNVAAVL RIDDDMTKDY TIFEAVQARD SGGNVILPTK KPQMDYFL

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR382125 Genomic DNA. Translation: CAG99490.1.
RefSeq	XP_454403.1. XM_454403.1.
3D structure databases
ProteinModelPortal	Q6CNT6.
SMR	Q6CNT6. Positions 1-307.
ModBase	Search...
Protein-protein interaction databases
STRING	28985.Q6CNT6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2893827.
KEGG	kla:KLLA0E10055g.
Phylogenomic databases
eggNOG	COG0639.
HOGENOM	HOG000172696.
KO	K01090.
OMA	TIVTVWS.
OrthoDB	EOG4HX88W.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP4C_KLULA

Accession

Primary (citable) accession number: Q6CNT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program