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Protein

Serine/threonine-protein phosphatase 4 catalytic subunit

Gene

PPH3

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Forms the histone H2A phosphatase complex in association with the regulatory subunits PSY2 and PSY4, which dephosphorylates H2AS128ph (gamma-H2A) that has been displaced from sites of DNA lesions in the double-stranded DNA break repair process. Dephosphorylation is necessary for efficient recovery from the DNA damage checkpoint (By similarity).By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi51 – 511Manganese 1By similarity
Metal bindingi53 – 531Manganese 1By similarity
Metal bindingi79 – 791Manganese 1By similarity
Metal bindingi79 – 791Manganese 2By similarity
Metal bindingi111 – 1111Manganese 2By similarity
Active sitei112 – 1121Proton donorBy similarity
Metal bindingi161 – 1611Manganese 2By similarity
Metal bindingi235 – 2351Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 4 catalytic subunit (EC:3.1.3.16)
Short name:
PP4C
Gene namesi
Name:PPH3
Ordered Locus Names:KLLA0E10032g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598: Chromosome E

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 308308Serine/threonine-protein phosphatase 4 catalytic subunitPRO_0000223651Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei308 – 3081Leucine methyl esterBy similarity

Keywords - PTMi

Methylation

Interactioni

Subunit structurei

Catalytic subunit of the histone H2A phosphatase complex (HTP-C) containing PPH3, PSY2 and PSY4.By similarity

Protein-protein interaction databases

STRINGi28985.Q6CNT6.

Structurei

3D structure databases

ProteinModelPortaliQ6CNT6.
SMRiQ6CNT6. Positions 1-307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172696.
InParanoidiQ6CNT6.
KOiK15423.
OMAiQCEPISE.
OrthoDBiEOG7FFN29.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6CNT6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMKLDEIIET LRQGKHVDED SIYSLCVMAQ ELLMNESNVT HVDTPVTICG
60 70 80 90 100
DIHGQLHDLL TLFAKSGGIE KNRYIFLGDF VDRGFYSLES FLLLVCYKLR
110 120 130 140 150
YPDRIVLIRG NHETRQITKV YGFYDEVVRK YGNSNVWRYC CEVFDYLPLG
160 170 180 190 200
AIVNNKVFCV HGGLSPDVLS INEIRTIDRK KEVPHEGAMC DLLWSDPEDV
210 220 230 240 250
DTWSLSPRGA GFLFGQNEVD KFLHTNSVEL IARAHQLVME GYKEMFDGGL
260 270 280 290 300
VTVWSAPNYC YRCGNVAAVL RIDDDMTKDY TIFEAVQARD SGGNVILPTK

KPQMDYFL
Length:308
Mass (Da):35,167
Last modified:August 16, 2004 - v1
Checksum:i5BF6D3BFDAB84CD8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382125 Genomic DNA. Translation: CAG99490.1.
RefSeqiXP_454403.1. XM_454403.1.

Genome annotation databases

GeneIDi2893827.
KEGGikla:KLLA0E10055g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382125 Genomic DNA. Translation: CAG99490.1.
RefSeqiXP_454403.1. XM_454403.1.

3D structure databases

ProteinModelPortaliQ6CNT6.
SMRiQ6CNT6. Positions 1-307.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi28985.Q6CNT6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2893827.
KEGGikla:KLLA0E10055g.

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172696.
InParanoidiQ6CNT6.
KOiK15423.
OMAiQCEPISE.
OrthoDBiEOG7FFN29.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPP4C_KLULA
AccessioniPrimary (citable) accession number: Q6CNT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: August 16, 2004
Last modified: January 7, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.