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Protein

Deoxyhypusine synthase

Gene

DYS1

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.By similarity

Catalytic activityi

[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.

Cofactori

NAD+By similarity

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei140 – 1401NADBy similarity
Binding sitei257 – 2571NADBy similarity
Binding sitei262 – 2621SpermidineBy similarity
Binding sitei304 – 3041NAD; via amide nitrogenBy similarity
Binding sitei309 – 3091SpermidineBy similarity
Active sitei350 – 3501NucleophileBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi108 – 1125NADBy similarity
Nucleotide bindingi134 – 1363NADBy similarity
Nucleotide bindingi329 – 3302NADBy similarity
Nucleotide bindingi363 – 3642NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Hypusine biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine synthase (EC:2.5.1.46)
Short name:
DHS
Gene namesi
Name:DYS1
Ordered Locus Names:KLLA0E12727g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome E

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 379379Deoxyhypusine synthasePRO_0000134484Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi284590.XP_454522.1.

Structurei

3D structure databases

ProteinModelPortaliQ6CNG7.
SMRiQ6CNG7. Positions 9-378.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni139 – 1402Spermidine bindingBy similarity
Regioni335 – 3373Spermidine bindingBy similarity
Regioni344 – 3507Spermidine bindingBy similarity

Sequence similaritiesi

Belongs to the deoxyhypusine synthase family.Curated

Phylogenomic databases

eggNOGiKOG2924. Eukaryota.
COG1899. LUCA.
HOGENOMiHOG000228838.
InParanoidiQ6CNG7.
KOiK00809.
OMAiDSMIGLQ.
OrthoDBiEOG092C3JEN.

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
InterProiIPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 2 hits.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6CNG7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNIDGKLPD ILSDAVLKQS VPVPDNFVKV EGIDYSKPES RNMRSKDLIK
60 70 80 90 100
SMATMGFQAS SLGKACDIID EMRSWRGEHR DKLEEHDRKG SFDNDGYQKT
110 120 130 140 150
TIFMGYTSNL ISSGLRETLR FLVQNKMVDA IVATAGGVEE DIIKCLADTY
160 170 180 190 200
LGEFSLPGKG LRDQGMNRIG NLLVPNDNYC KFEEWIVPIL DKMLEEQENY
210 220 230 240 250
VESQGKDCLD SNFDQDSPVW TPSKLIRRLG KEINDESSVL YWAYKNDIPI
260 270 280 290 300
FCPAVTDGSI GDMLFFHTFK ASPKQLRLDI VSDIRKINSM SMEASRAGMI
310 320 330 340 350
ILGGGLIKHH IANACLMRNG ADYAVYINTG QEFDGSDAGA RPDEAVSWGK
360 370
IKADAKSVKV YADATVVFPL VVAATFASE
Length:379
Mass (Da):42,074
Last modified:August 16, 2004 - v1
Checksum:iCFB6DB4343729C0E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382125 Genomic DNA. Translation: CAG99609.1.
RefSeqiXP_454522.1. XM_454522.1.

Genome annotation databases

EnsemblFungiiCAG99609; CAG99609; KLLA0_E12695g.
GeneIDi2894123.
KEGGikla:KLLA0E12695g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382125 Genomic DNA. Translation: CAG99609.1.
RefSeqiXP_454522.1. XM_454522.1.

3D structure databases

ProteinModelPortaliQ6CNG7.
SMRiQ6CNG7. Positions 9-378.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi284590.XP_454522.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAG99609; CAG99609; KLLA0_E12695g.
GeneIDi2894123.
KEGGikla:KLLA0E12695g.

Phylogenomic databases

eggNOGiKOG2924. Eukaryota.
COG1899. LUCA.
HOGENOMiHOG000228838.
InParanoidiQ6CNG7.
KOiK00809.
OMAiDSMIGLQ.
OrthoDBiEOG092C3JEN.

Enzyme and pathway databases

UniPathwayiUPA00354.

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
InterProiIPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 2 hits.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDHYS_KLULA
AccessioniPrimary (citable) accession number: Q6CNG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: August 16, 2004
Last modified: September 7, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.